Enhancing the Biodiesel Production by Improving the Yield of Lipids in Wild Strain by Inducing Nitrogen Ion Mutation in <i>Rhodotorula mucilaginosa</i>

The overconsumption of energy results in the depletion of fossil fuels. Generally, biodiesels are produced from wastes of animal fats and vegetable oils. In this study, we have tried to produce biodiesel from both the wild strain and ion beam mutated strain and compared the concentration of lipids p...

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Main Authors: Joseph Antony Sundarsingh Tensingh, Vijayalakshmi Shankar
Format: Article
Language:English
Published: MDPI AG 2023-09-01
Series:Microbiology Research
Subjects:
Online Access:https://www.mdpi.com/2036-7481/14/3/96
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author Joseph Antony Sundarsingh Tensingh
Vijayalakshmi Shankar
author_facet Joseph Antony Sundarsingh Tensingh
Vijayalakshmi Shankar
author_sort Joseph Antony Sundarsingh Tensingh
collection DOAJ
description The overconsumption of energy results in the depletion of fossil fuels. Generally, biodiesels are produced from wastes of animal fats and vegetable oils. In this study, we have tried to produce biodiesel from both the wild strain and ion beam mutated strain and compared the concentration of lipids produced from both the strains and their properties. Lipids were extracted from microbes using the Bligh and Dyer method and analyzed using gas chromatography and mass spectrophotometry (GCMS) and Fourier-transform infrared (FTIR) spectroscopy. Extracted lipids (free fatty acids) were converted into biodiesel (fatty acid methyl esters) using a base catalyst. The end product biodiesel was characterized and analyzed based on ASTM standards.
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spelling doaj.art-1cc68ebbc799494b99fcdb0ea003c85a2023-11-19T11:58:39ZengMDPI AGMicrobiology Research2036-74812023-09-011431413142610.3390/microbiolres14030096Enhancing the Biodiesel Production by Improving the Yield of Lipids in Wild Strain by Inducing Nitrogen Ion Mutation in <i>Rhodotorula mucilaginosa</i>Joseph Antony Sundarsingh Tensingh0Vijayalakshmi Shankar1School of Biosciences and Technology, Vellore Institute of Technology, Vellore 632014, IndiaSchool of Biosciences and Technology, Vellore Institute of Technology, Vellore 632014, IndiaThe overconsumption of energy results in the depletion of fossil fuels. Generally, biodiesels are produced from wastes of animal fats and vegetable oils. In this study, we have tried to produce biodiesel from both the wild strain and ion beam mutated strain and compared the concentration of lipids produced from both the strains and their properties. Lipids were extracted from microbes using the Bligh and Dyer method and analyzed using gas chromatography and mass spectrophotometry (GCMS) and Fourier-transform infrared (FTIR) spectroscopy. Extracted lipids (free fatty acids) were converted into biodiesel (fatty acid methyl esters) using a base catalyst. The end product biodiesel was characterized and analyzed based on ASTM standards.https://www.mdpi.com/2036-7481/14/3/96microorganismion beam radiationGC-MSFTIRbiodiesel
spellingShingle Joseph Antony Sundarsingh Tensingh
Vijayalakshmi Shankar
Enhancing the Biodiesel Production by Improving the Yield of Lipids in Wild Strain by Inducing Nitrogen Ion Mutation in <i>Rhodotorula mucilaginosa</i>
Microbiology Research
microorganism
ion beam radiation
GC-MS
FTIR
biodiesel
title Enhancing the Biodiesel Production by Improving the Yield of Lipids in Wild Strain by Inducing Nitrogen Ion Mutation in <i>Rhodotorula mucilaginosa</i>
title_full Enhancing the Biodiesel Production by Improving the Yield of Lipids in Wild Strain by Inducing Nitrogen Ion Mutation in <i>Rhodotorula mucilaginosa</i>
title_fullStr Enhancing the Biodiesel Production by Improving the Yield of Lipids in Wild Strain by Inducing Nitrogen Ion Mutation in <i>Rhodotorula mucilaginosa</i>
title_full_unstemmed Enhancing the Biodiesel Production by Improving the Yield of Lipids in Wild Strain by Inducing Nitrogen Ion Mutation in <i>Rhodotorula mucilaginosa</i>
title_short Enhancing the Biodiesel Production by Improving the Yield of Lipids in Wild Strain by Inducing Nitrogen Ion Mutation in <i>Rhodotorula mucilaginosa</i>
title_sort enhancing the biodiesel production by improving the yield of lipids in wild strain by inducing nitrogen ion mutation in i rhodotorula mucilaginosa i
topic microorganism
ion beam radiation
GC-MS
FTIR
biodiesel
url https://www.mdpi.com/2036-7481/14/3/96
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