Bee pollen in zebrafish diet affects intestinal microbiota composition and skin cutaneous melanoma development
Abstract Bee pollen is recommended as dietary supplement due to immunostimulating functions including antioxidant, anti-inflammatory and anti-carcinogenic properties. Nevertheless, the effectiveness of such properties is still not well understood. As diet can be associated with animal performance, m...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14245-3 |
| _version_ | 1811343398372638720 |
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| author | Isabela M. Di Chiacchio Elena Gómez-Abenza Isadora M. Paiva Danilo J. M. de Abreu Juan Francisco Rodríguez-Vidal Elisângela E. N. Carvalho Stephan M. Carvalho Luis David Solis-Murgas Victoriano Mulero |
| author_facet | Isabela M. Di Chiacchio Elena Gómez-Abenza Isadora M. Paiva Danilo J. M. de Abreu Juan Francisco Rodríguez-Vidal Elisângela E. N. Carvalho Stephan M. Carvalho Luis David Solis-Murgas Victoriano Mulero |
| author_sort | Isabela M. Di Chiacchio |
| collection | DOAJ |
| description | Abstract Bee pollen is recommended as dietary supplement due to immunostimulating functions including antioxidant, anti-inflammatory and anti-carcinogenic properties. Nevertheless, the effectiveness of such properties is still not well understood. As diet can be associated with animal performance, microbiota modulation and potentially factor for cancer, this study aimed to analyze if bee pollen could influence growth, gut microbial and skin cutaneous melanoma development in zebrafish. Control diets based on commercial flakes and Artemia were compared with the same diet supplemented with bee pollen. Fish weight gain, increased length, intestinal bacteria metagenomics analysis, serum amyloid A gene expression and cutaneous melanoma transplantation assays were performed. Bee pollen affected microbiota composition and melanoma development. Differential abundance revealed higher abundance in the control group for Aeromonadaceae family, Aeromonas and Pseudomonas genus, A. sobria, A. schubertii, A. jandaei and P. alcaligenes species compared with pollen diet group. Pollen group presented higher abundance for Chromobacterium genus and for Gemmobacter aquaticus, Flavobacterium succinicans and Bifidobacterium breve compared with control group. Unexpectedly, fish fed with bee pollen showed higher tumor growth rate and larger tumor size than control group. This is the first study to report intestinal microbial changes and no protective cancer properties after bee pollen administration. |
| first_indexed | 2024-04-13T19:28:42Z |
| format | Article |
| id | doaj.art-1cc84e42725d4c6780b39c660fc42d3f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T19:28:42Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1cc84e42725d4c6780b39c660fc42d3f2022-12-22T02:33:14ZengNature PortfolioScientific Reports2045-23222022-06-0112111810.1038/s41598-022-14245-3Bee pollen in zebrafish diet affects intestinal microbiota composition and skin cutaneous melanoma developmentIsabela M. Di Chiacchio0Elena Gómez-Abenza1Isadora M. Paiva2Danilo J. M. de Abreu3Juan Francisco Rodríguez-Vidal4Elisângela E. N. Carvalho5Stephan M. Carvalho6Luis David Solis-Murgas7Victoriano Mulero8Programa de Pós-graduação em Ciências Veterinárias-FZMV, Universidade Federal de Lavras, UFLADepartamento de Biología Celular e Histología, Facultad de Biología, Universidad de Murcia. IMIB-Arrixaca. CIBERERLaboratório de Genética Animal e Humana, Departamento de Genética, Ecologia e Evolução, Universidade Federal de Minas Gerais, UFMGPrograma de Pós-graduação em Microbiologia Agrícola-ICN, Universidade Federal de Lavras, UFLADepartamento de Biología Celular e Histología, Facultad de Biología, Universidad de Murcia. IMIB-Arrixaca. CIBERERDepartamento de Ciência dos Alimentos-ESAL, Universidade Federal de Lavras, UFLAPrograma de Pós-graduação em Entomologia-ESAL, Universidade Federal de Lavras, UFLAPrograma de Pós-graduação em Ciências Veterinárias-FZMV, Universidade Federal de Lavras, UFLADepartamento de Biología Celular e Histología, Facultad de Biología, Universidad de Murcia. IMIB-Arrixaca. CIBERERAbstract Bee pollen is recommended as dietary supplement due to immunostimulating functions including antioxidant, anti-inflammatory and anti-carcinogenic properties. Nevertheless, the effectiveness of such properties is still not well understood. As diet can be associated with animal performance, microbiota modulation and potentially factor for cancer, this study aimed to analyze if bee pollen could influence growth, gut microbial and skin cutaneous melanoma development in zebrafish. Control diets based on commercial flakes and Artemia were compared with the same diet supplemented with bee pollen. Fish weight gain, increased length, intestinal bacteria metagenomics analysis, serum amyloid A gene expression and cutaneous melanoma transplantation assays were performed. Bee pollen affected microbiota composition and melanoma development. Differential abundance revealed higher abundance in the control group for Aeromonadaceae family, Aeromonas and Pseudomonas genus, A. sobria, A. schubertii, A. jandaei and P. alcaligenes species compared with pollen diet group. Pollen group presented higher abundance for Chromobacterium genus and for Gemmobacter aquaticus, Flavobacterium succinicans and Bifidobacterium breve compared with control group. Unexpectedly, fish fed with bee pollen showed higher tumor growth rate and larger tumor size than control group. This is the first study to report intestinal microbial changes and no protective cancer properties after bee pollen administration.https://doi.org/10.1038/s41598-022-14245-3 |
| spellingShingle | Isabela M. Di Chiacchio Elena Gómez-Abenza Isadora M. Paiva Danilo J. M. de Abreu Juan Francisco Rodríguez-Vidal Elisângela E. N. Carvalho Stephan M. Carvalho Luis David Solis-Murgas Victoriano Mulero Bee pollen in zebrafish diet affects intestinal microbiota composition and skin cutaneous melanoma development Scientific Reports |
| title | Bee pollen in zebrafish diet affects intestinal microbiota composition and skin cutaneous melanoma development |
| title_full | Bee pollen in zebrafish diet affects intestinal microbiota composition and skin cutaneous melanoma development |
| title_fullStr | Bee pollen in zebrafish diet affects intestinal microbiota composition and skin cutaneous melanoma development |
| title_full_unstemmed | Bee pollen in zebrafish diet affects intestinal microbiota composition and skin cutaneous melanoma development |
| title_short | Bee pollen in zebrafish diet affects intestinal microbiota composition and skin cutaneous melanoma development |
| title_sort | bee pollen in zebrafish diet affects intestinal microbiota composition and skin cutaneous melanoma development |
| url | https://doi.org/10.1038/s41598-022-14245-3 |
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