Revealing the Regulatory Mechanism of lncRNA-LMEP on Melanin Deposition Based on High-Throughput Sequencing in Xichuan Chicken Skin
The therapeutic, medicinal, and nourishing properties of black-bone chickens are highly regarded by consumers in China. However, some birds may have yellow skin (YS) or light skin rather than black skin (BS), which causes economic losses every year. Long noncoding RNAs (lncRNAs) are widely present i...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/11/2143 |
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| author | Pengwei Zhang Yanfang Cao Yawei Fu Huiyuan Zhu Shuohui Xu Yanhua Zhang Wenting Li Guirong Sun Ruirui Jiang Ruili Han Hong Li Guoxi Li Yadong Tian Xiaojun Liu Xiangtao Kang Donghua Li |
| author_facet | Pengwei Zhang Yanfang Cao Yawei Fu Huiyuan Zhu Shuohui Xu Yanhua Zhang Wenting Li Guirong Sun Ruirui Jiang Ruili Han Hong Li Guoxi Li Yadong Tian Xiaojun Liu Xiangtao Kang Donghua Li |
| author_sort | Pengwei Zhang |
| collection | DOAJ |
| description | The therapeutic, medicinal, and nourishing properties of black-bone chickens are highly regarded by consumers in China. However, some birds may have yellow skin (YS) or light skin rather than black skin (BS), which causes economic losses every year. Long noncoding RNAs (lncRNAs) are widely present in living organisms, and they perform various biological functions. Many genes associated with BS pigmentation have been discovered, but the lncRNAs involved and their detailed mechanisms have remained untested. We detected 56 differentially expressed lncRNAs from the RNA-seq of dorsal skin (BS versus YS) and found that <i>TCONS_00054154</i> plays a vital role in melanogenesis by the combined analysis of lncRNAs and mRNAs. We found that the full length of the <i>TCONS_00054154</i> sequence was 3093 bp by RACE PCR, and we named it <i>LMEP</i>. Moreover, a subcellular localization analysis identified that <i>LMEP</i> is mainly present in the cytoplasm. After the overexpression and the interference with <i>LMEP</i>, the tyrosinase content significantly increased and decreased, respectively (<i>p</i> < 0.05). In summary, we identified the important lncRNAs of chicken skin pigmentation and initially determined the effect of <i>LMEP</i> on melanin deposition. |
| first_indexed | 2024-03-09T18:19:20Z |
| format | Article |
| id | doaj.art-1ccca69281ac46bf8b5b6154c0680756 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T18:19:20Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-1ccca69281ac46bf8b5b6154c06807562023-11-24T08:26:58ZengMDPI AGGenes2073-44252022-11-011311214310.3390/genes13112143Revealing the Regulatory Mechanism of lncRNA-LMEP on Melanin Deposition Based on High-Throughput Sequencing in Xichuan Chicken SkinPengwei Zhang0Yanfang Cao1Yawei Fu2Huiyuan Zhu3Shuohui Xu4Yanhua Zhang5Wenting Li6Guirong Sun7Ruirui Jiang8Ruili Han9Hong Li10Guoxi Li11Yadong Tian12Xiaojun Liu13Xiangtao Kang14Donghua Li15College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaThe therapeutic, medicinal, and nourishing properties of black-bone chickens are highly regarded by consumers in China. However, some birds may have yellow skin (YS) or light skin rather than black skin (BS), which causes economic losses every year. Long noncoding RNAs (lncRNAs) are widely present in living organisms, and they perform various biological functions. Many genes associated with BS pigmentation have been discovered, but the lncRNAs involved and their detailed mechanisms have remained untested. We detected 56 differentially expressed lncRNAs from the RNA-seq of dorsal skin (BS versus YS) and found that <i>TCONS_00054154</i> plays a vital role in melanogenesis by the combined analysis of lncRNAs and mRNAs. We found that the full length of the <i>TCONS_00054154</i> sequence was 3093 bp by RACE PCR, and we named it <i>LMEP</i>. Moreover, a subcellular localization analysis identified that <i>LMEP</i> is mainly present in the cytoplasm. After the overexpression and the interference with <i>LMEP</i>, the tyrosinase content significantly increased and decreased, respectively (<i>p</i> < 0.05). In summary, we identified the important lncRNAs of chicken skin pigmentation and initially determined the effect of <i>LMEP</i> on melanin deposition.https://www.mdpi.com/2073-4425/13/11/2143skin pigmentationRNA-seqmelaninchickenlncRNA-LMEP |
| spellingShingle | Pengwei Zhang Yanfang Cao Yawei Fu Huiyuan Zhu Shuohui Xu Yanhua Zhang Wenting Li Guirong Sun Ruirui Jiang Ruili Han Hong Li Guoxi Li Yadong Tian Xiaojun Liu Xiangtao Kang Donghua Li Revealing the Regulatory Mechanism of lncRNA-LMEP on Melanin Deposition Based on High-Throughput Sequencing in Xichuan Chicken Skin Genes skin pigmentation RNA-seq melanin chicken lncRNA-LMEP |
| title | Revealing the Regulatory Mechanism of lncRNA-LMEP on Melanin Deposition Based on High-Throughput Sequencing in Xichuan Chicken Skin |
| title_full | Revealing the Regulatory Mechanism of lncRNA-LMEP on Melanin Deposition Based on High-Throughput Sequencing in Xichuan Chicken Skin |
| title_fullStr | Revealing the Regulatory Mechanism of lncRNA-LMEP on Melanin Deposition Based on High-Throughput Sequencing in Xichuan Chicken Skin |
| title_full_unstemmed | Revealing the Regulatory Mechanism of lncRNA-LMEP on Melanin Deposition Based on High-Throughput Sequencing in Xichuan Chicken Skin |
| title_short | Revealing the Regulatory Mechanism of lncRNA-LMEP on Melanin Deposition Based on High-Throughput Sequencing in Xichuan Chicken Skin |
| title_sort | revealing the regulatory mechanism of lncrna lmep on melanin deposition based on high throughput sequencing in xichuan chicken skin |
| topic | skin pigmentation RNA-seq melanin chicken lncRNA-LMEP |
| url | https://www.mdpi.com/2073-4425/13/11/2143 |
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