Chemical Composition of <i>Impatiens textori</i> Miq. Flower Absolute and Its Potential Wound Repair and Anti-Melanogenesis-Promoting Activities in Skin Cells
<i>Impatiens textori</i> Miq. (ITM; family Balsaminaceae) is a traditional medicinal plant with many biological activities, which include anti-allergic, anti-inflammatory, and anti-pruritic properties. However, it remains to be determined whether ITM affects biological activities in the...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/15/11/1397 |
| _version_ | 1797464204241797120 |
|---|---|
| author | Yu Rim Won Kyung Jong Won Do Yoon Kim Mi Jung Kim Bok Sil Hong Hwan Myung Lee |
| author_facet | Yu Rim Won Kyung Jong Won Do Yoon Kim Mi Jung Kim Bok Sil Hong Hwan Myung Lee |
| author_sort | Yu Rim Won |
| collection | DOAJ |
| description | <i>Impatiens textori</i> Miq. (ITM; family Balsaminaceae) is a traditional medicinal plant with many biological activities, which include anti-allergic, anti-inflammatory, and anti-pruritic properties. However, it remains to be determined whether ITM affects biological activities in the skin. Thus, we investigated the effects of ITM flower absolute (ITMFAb) extract on the biological activities of skin, especially those related to skin wound repair and whitening. ITMFAb was extracted with hexane, and its composition was determined through GC/MS. The biological activities of ITMFAb on HaCaT keratinocytes and B16BL6 melanoma cells were analyzed using a water-soluble tetrazolium salt, 5-bromo-2′-deoxyuridine incorporation, a Boyden chamber, an ELISA, a sprouting assay, and by immunoblotting. These analyses were performed in a range of ITMFAb concentrations that did not inhibit the viability of the cells (HaCaT, ≤400 µg/mL; B16BL6, ≤200 µg/m). Forty components were identified in ITMFAb. ITMFAb stimulated proliferation, migration, sprout outgrowth, and type I and IV collagen synthesis and upregulated the activations of ERK1/2, JNK, p38 MAPK, and AKT in HaCaT cells. In addition, ITMFAb attenuated the serum-induced proliferation of B16BL6 cells. ITMFAb inhibited melanin synthesis, tyrosinase activity, and expressions of MITF and tyrosinase in α-MSH-exposed B16BL6 cells. These findings indicate that ITMFAb has beneficial effects on wound repairing and whitening-linked responses in the skin and suggest the potential use of ITMFAb as a natural material for the development of skin wound repair and whitening agents. |
| first_indexed | 2024-03-09T18:04:33Z |
| format | Article |
| id | doaj.art-1cccc7d2f6d046bda38c538b9d341fbe |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T18:04:33Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-1cccc7d2f6d046bda38c538b9d341fbe2023-11-24T09:35:06ZengMDPI AGPharmaceuticals1424-82472022-11-011511139710.3390/ph15111397Chemical Composition of <i>Impatiens textori</i> Miq. Flower Absolute and Its Potential Wound Repair and Anti-Melanogenesis-Promoting Activities in Skin CellsYu Rim Won0Kyung Jong Won1Do Yoon Kim2Mi Jung Kim3Bok Sil Hong4Hwan Myung Lee5Division of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan 31499, KoreaDepartment of Physiology and Medical Science, School of Medicine, Konkuk University, Seoul 05029, KoreaDivision of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan 31499, KoreaDivision of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan 31499, KoreaDepartment of Nursing, Life Science Research Center, Cheju Halla University, Jeju 63092, KoreaDivision of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan 31499, Korea<i>Impatiens textori</i> Miq. (ITM; family Balsaminaceae) is a traditional medicinal plant with many biological activities, which include anti-allergic, anti-inflammatory, and anti-pruritic properties. However, it remains to be determined whether ITM affects biological activities in the skin. Thus, we investigated the effects of ITM flower absolute (ITMFAb) extract on the biological activities of skin, especially those related to skin wound repair and whitening. ITMFAb was extracted with hexane, and its composition was determined through GC/MS. The biological activities of ITMFAb on HaCaT keratinocytes and B16BL6 melanoma cells were analyzed using a water-soluble tetrazolium salt, 5-bromo-2′-deoxyuridine incorporation, a Boyden chamber, an ELISA, a sprouting assay, and by immunoblotting. These analyses were performed in a range of ITMFAb concentrations that did not inhibit the viability of the cells (HaCaT, ≤400 µg/mL; B16BL6, ≤200 µg/m). Forty components were identified in ITMFAb. ITMFAb stimulated proliferation, migration, sprout outgrowth, and type I and IV collagen synthesis and upregulated the activations of ERK1/2, JNK, p38 MAPK, and AKT in HaCaT cells. In addition, ITMFAb attenuated the serum-induced proliferation of B16BL6 cells. ITMFAb inhibited melanin synthesis, tyrosinase activity, and expressions of MITF and tyrosinase in α-MSH-exposed B16BL6 cells. These findings indicate that ITMFAb has beneficial effects on wound repairing and whitening-linked responses in the skin and suggest the potential use of ITMFAb as a natural material for the development of skin wound repair and whitening agents.https://www.mdpi.com/1424-8247/15/11/1397<i>Impatiens textori</i> Miq.absoluteskin wound healingmelanin biosynthesiskeratinocytemelanoma cell |
| spellingShingle | Yu Rim Won Kyung Jong Won Do Yoon Kim Mi Jung Kim Bok Sil Hong Hwan Myung Lee Chemical Composition of <i>Impatiens textori</i> Miq. Flower Absolute and Its Potential Wound Repair and Anti-Melanogenesis-Promoting Activities in Skin Cells Pharmaceuticals <i>Impatiens textori</i> Miq. absolute skin wound healing melanin biosynthesis keratinocyte melanoma cell |
| title | Chemical Composition of <i>Impatiens textori</i> Miq. Flower Absolute and Its Potential Wound Repair and Anti-Melanogenesis-Promoting Activities in Skin Cells |
| title_full | Chemical Composition of <i>Impatiens textori</i> Miq. Flower Absolute and Its Potential Wound Repair and Anti-Melanogenesis-Promoting Activities in Skin Cells |
| title_fullStr | Chemical Composition of <i>Impatiens textori</i> Miq. Flower Absolute and Its Potential Wound Repair and Anti-Melanogenesis-Promoting Activities in Skin Cells |
| title_full_unstemmed | Chemical Composition of <i>Impatiens textori</i> Miq. Flower Absolute and Its Potential Wound Repair and Anti-Melanogenesis-Promoting Activities in Skin Cells |
| title_short | Chemical Composition of <i>Impatiens textori</i> Miq. Flower Absolute and Its Potential Wound Repair and Anti-Melanogenesis-Promoting Activities in Skin Cells |
| title_sort | chemical composition of i impatiens textori i miq flower absolute and its potential wound repair and anti melanogenesis promoting activities in skin cells |
| topic | <i>Impatiens textori</i> Miq. absolute skin wound healing melanin biosynthesis keratinocyte melanoma cell |
| url | https://www.mdpi.com/1424-8247/15/11/1397 |
| work_keys_str_mv | AT yurimwon chemicalcompositionofiimpatienstextoriimiqflowerabsoluteanditspotentialwoundrepairandantimelanogenesispromotingactivitiesinskincells AT kyungjongwon chemicalcompositionofiimpatienstextoriimiqflowerabsoluteanditspotentialwoundrepairandantimelanogenesispromotingactivitiesinskincells AT doyoonkim chemicalcompositionofiimpatienstextoriimiqflowerabsoluteanditspotentialwoundrepairandantimelanogenesispromotingactivitiesinskincells AT mijungkim chemicalcompositionofiimpatienstextoriimiqflowerabsoluteanditspotentialwoundrepairandantimelanogenesispromotingactivitiesinskincells AT boksilhong chemicalcompositionofiimpatienstextoriimiqflowerabsoluteanditspotentialwoundrepairandantimelanogenesispromotingactivitiesinskincells AT hwanmyunglee chemicalcompositionofiimpatienstextoriimiqflowerabsoluteanditspotentialwoundrepairandantimelanogenesispromotingactivitiesinskincells |