Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract
The COVID-19 pandemic has currently created an unprecedented threat to human society and global health. A rapid mass vaccination to create herd immunity against SARS-CoV-2 is a crucial measure to ease the spread of this disease. Here, we investigated the immunogenicity of a SARS-CoV-2 subunit vaccin...
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MDPI AG
2021-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/11/2202 |
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| author | Tuksin Jearanaiwitayakul Suttikarn Apichirapokey Runglawan Chawengkirttikul Jitra Limthongkul Mathurin Seesen Phissinee Jakaew Sakalin Trisiriwanich Sompong Sapsutthipas Panya Sunintaboon Sukathida Ubol |
| author_facet | Tuksin Jearanaiwitayakul Suttikarn Apichirapokey Runglawan Chawengkirttikul Jitra Limthongkul Mathurin Seesen Phissinee Jakaew Sakalin Trisiriwanich Sompong Sapsutthipas Panya Sunintaboon Sukathida Ubol |
| author_sort | Tuksin Jearanaiwitayakul |
| collection | DOAJ |
| description | The COVID-19 pandemic has currently created an unprecedented threat to human society and global health. A rapid mass vaccination to create herd immunity against SARS-CoV-2 is a crucial measure to ease the spread of this disease. Here, we investigated the immunogenicity of a SARS-CoV-2 subunit vaccine candidate, a SARS-CoV-2 spike glycoprotein encapsulated in <i>N</i>,<i>N</i>,<i>N</i>-trimethyl chitosan particles or S-TMC NPs. Upon intraperitoneal immunization, S-TMC NP-immunized mice elicited a stronger systemic antibody response, with neutralizing capacity against SARS-CoV-2, than mice receiving the soluble form of S-glycoprotein. S-TMC NPs were able to stimulate the circulating IgG and IgA as found in SARS-CoV-2-infected patients. In addition, spike-specific T cell responses were drastically activated in S-TMC NP-immunized mice. Surprisingly, administration of S-TMC NPs via the intraperitoneal route also stimulated SARS-CoV-2-specific immune responses in the respiratory tract, which were demonstrated by the presence of high levels of SARS-CoV-2-specific IgG and IgA in the lung homogenates and bronchoalveolar lavages of the immunized mice. We found that peritoneal immunization with spike nanospheres stimulates both systemic and respiratory mucosal immunity. |
| first_indexed | 2024-03-10T04:58:52Z |
| format | Article |
| id | doaj.art-1ccef95447414f188efbf174b17bb7f1 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T04:58:52Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1ccef95447414f188efbf174b17bb7f12023-11-23T01:56:38ZengMDPI AGViruses1999-49152021-11-011311220210.3390/v13112202Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory TractTuksin Jearanaiwitayakul0Suttikarn Apichirapokey1Runglawan Chawengkirttikul2Jitra Limthongkul3Mathurin Seesen4Phissinee Jakaew5Sakalin Trisiriwanich6Sompong Sapsutthipas7Panya Sunintaboon8Sukathida Ubol9Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandInstitute of Biological Products, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, ThailandInstitute of Biological Products, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, ThailandDepartment of Chemistry, Faculty of Science, Mahidol University, Salaya, Nakornpatom 73170, ThailandDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandThe COVID-19 pandemic has currently created an unprecedented threat to human society and global health. A rapid mass vaccination to create herd immunity against SARS-CoV-2 is a crucial measure to ease the spread of this disease. Here, we investigated the immunogenicity of a SARS-CoV-2 subunit vaccine candidate, a SARS-CoV-2 spike glycoprotein encapsulated in <i>N</i>,<i>N</i>,<i>N</i>-trimethyl chitosan particles or S-TMC NPs. Upon intraperitoneal immunization, S-TMC NP-immunized mice elicited a stronger systemic antibody response, with neutralizing capacity against SARS-CoV-2, than mice receiving the soluble form of S-glycoprotein. S-TMC NPs were able to stimulate the circulating IgG and IgA as found in SARS-CoV-2-infected patients. In addition, spike-specific T cell responses were drastically activated in S-TMC NP-immunized mice. Surprisingly, administration of S-TMC NPs via the intraperitoneal route also stimulated SARS-CoV-2-specific immune responses in the respiratory tract, which were demonstrated by the presence of high levels of SARS-CoV-2-specific IgG and IgA in the lung homogenates and bronchoalveolar lavages of the immunized mice. We found that peritoneal immunization with spike nanospheres stimulates both systemic and respiratory mucosal immunity.https://www.mdpi.com/1999-4915/13/11/2202SARS-CoV-2 spike glycoproteinCOVID-19 vaccineadjuvant delivery particlesperitoneal immunizationmucosal immunity |
| spellingShingle | Tuksin Jearanaiwitayakul Suttikarn Apichirapokey Runglawan Chawengkirttikul Jitra Limthongkul Mathurin Seesen Phissinee Jakaew Sakalin Trisiriwanich Sompong Sapsutthipas Panya Sunintaboon Sukathida Ubol Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract Viruses SARS-CoV-2 spike glycoprotein COVID-19 vaccine adjuvant delivery particles peritoneal immunization mucosal immunity |
| title | Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract |
| title_full | Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract |
| title_fullStr | Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract |
| title_full_unstemmed | Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract |
| title_short | Peritoneal Administration of a Subunit Vaccine Encapsulated in a Nanodelivery System Not Only Augments Systemic Responses against SARS-CoV-2 but Also Stimulates Responses in the Respiratory Tract |
| title_sort | peritoneal administration of a subunit vaccine encapsulated in a nanodelivery system not only augments systemic responses against sars cov 2 but also stimulates responses in the respiratory tract |
| topic | SARS-CoV-2 spike glycoprotein COVID-19 vaccine adjuvant delivery particles peritoneal immunization mucosal immunity |
| url | https://www.mdpi.com/1999-4915/13/11/2202 |
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