Comparative Anti-malarial and Toxicological Properties of the Stem Bark Extracts of Nauclea latifolia and Terminalia glaucescens Against Plasmodium berghei-infected Mice
Background: Anti-plasmodial activities of the methanol stem bark extracts of Nauclea latifolia, and Terminalia glaucescens were investigated in mice infected with Plasmodium berghei. Methods: A total of 24 Plasmodium berghei infected (P. berghei infected) mice were divided into 8 groups of 3 each. G...
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2020-01-01
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| Series: | Iranian Journal of Toxicology |
| Subjects: | |
| Online Access: | http://ijt.arakmu.ac.ir/article-1-770-en.html |
| _version_ | 1818987659405557760 |
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| author | Eustace Bonghan Berinyuy Mann Abdullahi Adamu Yusuf Kabiru Emmanuel Olofu Ogbadoyi |
| author_facet | Eustace Bonghan Berinyuy Mann Abdullahi Adamu Yusuf Kabiru Emmanuel Olofu Ogbadoyi |
| author_sort | Eustace Bonghan Berinyuy |
| collection | DOAJ |
| description | Background: Anti-plasmodial activities of the methanol stem bark extracts of Nauclea latifolia, and Terminalia glaucescens were investigated in mice infected with Plasmodium berghei.
Methods: A total of 24 Plasmodium berghei infected (P. berghei infected) mice were divided into 8 groups of 3 each. Groups A-F were given 100, 300 or 500 mg/kg of either extracts. Groups G and H received 2 mL normal saline (negative control) and 5 mg/kg of chloroquine (positive control), respectively. The drugs and extracts were administered orally once daily for five days.
Results: Alkaloids and flavonoids were the most and abundant metabolites in the extracts, respectively. The extract of Nauclea latifolia (N. latifolia) and Terminalia glaucescens (T. glauscecens) had Median Lethal Dose LD50 of >5000 mg/kg and 3808 mg/kg, respectively. In vivo anti-plasmodial studies revealed that the highest suppression (66.79% and 65.37%) and mean survival days (27.67±1.45 and 30.33±0.33) were recorded for the groups treated with 500 mg/kg N. latifolia or T. glaucescens, respectively. The infected but untreated groups survived only for 9.33±0.88 days while chloroquine treated groups lived for 31.33±0.88 days. The body weight and Packed Cell Volume (PCV) of rats treated with 500 mg/kg. N. latifolia or T. glaucescens significantly increased (P<0.05) compared to those in the infected but untreated groups. There was a significant loss (P<0.05) in body weight and PCV of the mice treated with 100 mg/kg of T. glaucescens compared to those in other treated groups.
Conclusion: The extracts exhibited anti-plasmodial activities in mice, therefore, they may be considered potential candidates for new anti-malarial agents. |
| first_indexed | 2024-12-20T19:10:12Z |
| format | Article |
| id | doaj.art-1cd050d6f48449b894312d0817a9aa3e |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-12-20T19:10:12Z |
| publishDate | 2020-01-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-1cd050d6f48449b894312d0817a9aa3e2022-12-21T19:29:14ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592020-01-01141918Comparative Anti-malarial and Toxicological Properties of the Stem Bark Extracts of Nauclea latifolia and Terminalia glaucescens Against Plasmodium berghei-infected MiceEustace Bonghan Berinyuy0Mann Abdullahi1Adamu Yusuf Kabiru2Emmanuel Olofu Ogbadoyi3 Department of Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde 1, Yaounde, Cameroon. Department of Chemistry, School of Life Sciences, Federal University of Technology, Minna, Nigeria. Department of Biochemistry, School of Life Sciences, Federal University of Technology, Minna, Nigeria. Department of Biochemistry, School of Life Sciences, Federal University of Technology, Minna, Nigeria. Background: Anti-plasmodial activities of the methanol stem bark extracts of Nauclea latifolia, and Terminalia glaucescens were investigated in mice infected with Plasmodium berghei. Methods: A total of 24 Plasmodium berghei infected (P. berghei infected) mice were divided into 8 groups of 3 each. Groups A-F were given 100, 300 or 500 mg/kg of either extracts. Groups G and H received 2 mL normal saline (negative control) and 5 mg/kg of chloroquine (positive control), respectively. The drugs and extracts were administered orally once daily for five days. Results: Alkaloids and flavonoids were the most and abundant metabolites in the extracts, respectively. The extract of Nauclea latifolia (N. latifolia) and Terminalia glaucescens (T. glauscecens) had Median Lethal Dose LD50 of >5000 mg/kg and 3808 mg/kg, respectively. In vivo anti-plasmodial studies revealed that the highest suppression (66.79% and 65.37%) and mean survival days (27.67±1.45 and 30.33±0.33) were recorded for the groups treated with 500 mg/kg N. latifolia or T. glaucescens, respectively. The infected but untreated groups survived only for 9.33±0.88 days while chloroquine treated groups lived for 31.33±0.88 days. The body weight and Packed Cell Volume (PCV) of rats treated with 500 mg/kg. N. latifolia or T. glaucescens significantly increased (P<0.05) compared to those in the infected but untreated groups. There was a significant loss (P<0.05) in body weight and PCV of the mice treated with 100 mg/kg of T. glaucescens compared to those in other treated groups. Conclusion: The extracts exhibited anti-plasmodial activities in mice, therefore, they may be considered potential candidates for new anti-malarial agents.http://ijt.arakmu.ac.ir/article-1-770-en.htmlphytochemicalstoxicityanti-malarial drugsnauclea latifoliaterminalia glaucescens |
| spellingShingle | Eustace Bonghan Berinyuy Mann Abdullahi Adamu Yusuf Kabiru Emmanuel Olofu Ogbadoyi Comparative Anti-malarial and Toxicological Properties of the Stem Bark Extracts of Nauclea latifolia and Terminalia glaucescens Against Plasmodium berghei-infected Mice Iranian Journal of Toxicology phytochemicals toxicity anti-malarial drugs nauclea latifolia terminalia glaucescens |
| title | Comparative Anti-malarial and Toxicological Properties of the Stem Bark Extracts of Nauclea latifolia and Terminalia glaucescens Against Plasmodium berghei-infected Mice |
| title_full | Comparative Anti-malarial and Toxicological Properties of the Stem Bark Extracts of Nauclea latifolia and Terminalia glaucescens Against Plasmodium berghei-infected Mice |
| title_fullStr | Comparative Anti-malarial and Toxicological Properties of the Stem Bark Extracts of Nauclea latifolia and Terminalia glaucescens Against Plasmodium berghei-infected Mice |
| title_full_unstemmed | Comparative Anti-malarial and Toxicological Properties of the Stem Bark Extracts of Nauclea latifolia and Terminalia glaucescens Against Plasmodium berghei-infected Mice |
| title_short | Comparative Anti-malarial and Toxicological Properties of the Stem Bark Extracts of Nauclea latifolia and Terminalia glaucescens Against Plasmodium berghei-infected Mice |
| title_sort | comparative anti malarial and toxicological properties of the stem bark extracts of nauclea latifolia and terminalia glaucescens against plasmodium berghei infected mice |
| topic | phytochemicals toxicity anti-malarial drugs nauclea latifolia terminalia glaucescens |
| url | http://ijt.arakmu.ac.ir/article-1-770-en.html |
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