Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts
A simple and rapid HPLC method for determination of chlorogenic acid (5-O-caffeoylquinic acid) in mate tea extracts was developed and validated. The chromatography used isocratic elution with a mobile phase of aqueous 1.5% acetic acid-methanol (85:15, v/v). The flow rate was 0.8 mL/min and detection...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/3/2518/ |
| _version_ | 1818967310382137344 |
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| author | Jelena Vladic Branislava Srdjenovic Zika Lepojevic Nevena Grujic Jan Sudji |
| author_facet | Jelena Vladic Branislava Srdjenovic Zika Lepojevic Nevena Grujic Jan Sudji |
| author_sort | Jelena Vladic |
| collection | DOAJ |
| description | A simple and rapid HPLC method for determination of chlorogenic acid (5-O-caffeoylquinic acid) in mate tea extracts was developed and validated. The chromatography used isocratic elution with a mobile phase of aqueous 1.5% acetic acid-methanol (85:15, v/v). The flow rate was 0.8 mL/min and detection by UV at 325 nm. The method showed good selectivity, accuracy, repeatability and robustness, with detection limit of 0.26 mg/L and recovery of 97.76%. The developed method was applied for the determination of chlorogenic acid in mate tea extracts obtained by ethanol extraction and liquid carbon dioxide extraction with ethanol as co-solvent. Different ethanol concentrations were used (40, 50 and 60%, v/v) and liquid CO2 extraction was performed at different pressures (50 and 100 bar) and constant temperature (27 ± 1 °C). Significant influence of extraction methods, conditions and solvent polarity on chlorogenic acid content, antioxidant activity and total phenolic and flavonoid content of mate tea extracts was established. The most efficient extraction solvent was liquid CO2 with aqueous ethanol (40%) as co-solvent using an extraction pressure of 100 bar. |
| first_indexed | 2024-12-20T13:46:46Z |
| format | Article |
| id | doaj.art-1cd1293308f8457fb9f32fa5d59e9546 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T13:46:46Z |
| publishDate | 2012-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1cd1293308f8457fb9f32fa5d59e95462022-12-21T19:38:38ZengMDPI AGMolecules1420-30492012-03-011732518252810.3390/molecules17032518Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea ExtractsJelena VladicBranislava SrdjenovicZika LepojevicNevena GrujicJan SudjiA simple and rapid HPLC method for determination of chlorogenic acid (5-O-caffeoylquinic acid) in mate tea extracts was developed and validated. The chromatography used isocratic elution with a mobile phase of aqueous 1.5% acetic acid-methanol (85:15, v/v). The flow rate was 0.8 mL/min and detection by UV at 325 nm. The method showed good selectivity, accuracy, repeatability and robustness, with detection limit of 0.26 mg/L and recovery of 97.76%. The developed method was applied for the determination of chlorogenic acid in mate tea extracts obtained by ethanol extraction and liquid carbon dioxide extraction with ethanol as co-solvent. Different ethanol concentrations were used (40, 50 and 60%, v/v) and liquid CO2 extraction was performed at different pressures (50 and 100 bar) and constant temperature (27 ± 1 °C). Significant influence of extraction methods, conditions and solvent polarity on chlorogenic acid content, antioxidant activity and total phenolic and flavonoid content of mate tea extracts was established. The most efficient extraction solvent was liquid CO2 with aqueous ethanol (40%) as co-solvent using an extraction pressure of 100 bar.http://www.mdpi.com/1420-3049/17/3/2518/matechlorogenic acidHPLC analysisDPPHtotal phenolic contenttotal flavonoid content |
| spellingShingle | Jelena Vladic Branislava Srdjenovic Zika Lepojevic Nevena Grujic Jan Sudji Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts Molecules mate chlorogenic acid HPLC analysis DPPH total phenolic content total flavonoid content |
| title | Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts |
| title_full | Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts |
| title_fullStr | Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts |
| title_full_unstemmed | Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts |
| title_short | Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts |
| title_sort | effects of different extraction methods and conditions on the phenolic composition of mate tea extracts |
| topic | mate chlorogenic acid HPLC analysis DPPH total phenolic content total flavonoid content |
| url | http://www.mdpi.com/1420-3049/17/3/2518/ |
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