The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage Polarization
Yaowei Xuan,1,* Lin Li,2,* Chenping Zhang,3,4 Min Zhang,1 Junkai Cao,1 Zhen Zhang3,4 1Department of Stomatology, The First Medical Centre, Chinese PLA General Hospital, Beijing, People’s Republic of China; 2State Key Laboratory of Military Stomatology & National Clinical Research...
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Dove Medical Press
2023-02-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/the-3d-printed-ordered-bredigite-scaffold-promotes-pro-healing-of-crit-peer-reviewed-fulltext-article-IJN |
| _version_ | 1828010401238876160 |
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| author | Xuan Y Li L Zhang C Zhang M Cao J Zhang Z |
| author_facet | Xuan Y Li L Zhang C Zhang M Cao J Zhang Z |
| author_sort | Xuan Y |
| collection | DOAJ |
| description | Yaowei Xuan,1,* Lin Li,2,* Chenping Zhang,3,4 Min Zhang,1 Junkai Cao,1 Zhen Zhang3,4 1Department of Stomatology, The First Medical Centre, Chinese PLA General Hospital, Beijing, People’s Republic of China; 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China; 3Department of Oral Maxillofacial-Head Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 4College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhen Zhang; Junkai Cao, Tel/Fax +86 153-1779-7335 ; +86 139-0129-0992, Email zz_nirvana@163.com; caojk301@163.comBackground: Repairing critical-sized bone defects secondary to traumatic or tumorous damage is a complex conundrum in clinical practice; in this case, artificial scaffolds exhibited preferable outcomes. Bredigite (BRT, Ca7MgSi4O16) bioceramic possesses excellent physicochemical properties and biological activity as a promising candidate for bone tissue engineering.Methods: Structurally ordered BRT (BRT-O) scaffolds were fabricated by a three-dimensional (3D) printing technique, and the random BRT (BRT-R) scaffolds and clinically available β-tricalcium phosphate (β-TCP) scaffolds were compared as control groups. Their physicochemical properties were characterized, and RAW 264.7 cells, bone marrow mesenchymal stem cells (BMSCs), and rat cranial critical-sized bone defect models were utilized for evaluating macrophage polarization and bone regeneration.Results: The BRT-O scaffolds exhibited regular morphology and homogeneous porosity. In addition, the BRT-O scaffolds released higher concentrations of ionic products based on coordinated biodegradability than the β-TCP scaffolds. In vitro, the BRT-O scaffolds facilitated RWA264.7 cells polarization to pro-healing M2 macrophage phenotype, whereas the BRT-R and β-TCP scaffolds stimulated more pro-inflammatory M1-type macrophages. A conditioned medium derived from macrophages seeding on the BRT-O scaffolds notably promoted the osteogenic lineage differentiation of BMSCs in vitro. The cell migration ability of BMSCs was significantly enhanced under the BRT-O-induced immune microenvironment. Moreover, in rat cranial critical-sized bone defect models, the BRT-O scaffolds group promoted new bone formation with a higher proportion of M2-type macrophage infiltration and expression of osteogenesis-related markers. Therefore, in vivo, BRT-O scaffolds play immunomodulatory roles in promoting critical-sized bone defects by enhancing the polarization of M2 macrophages.Conclusion: 3D-printed BRT-O scaffolds can be a promising option for bone tissue engineering, at least partly through macrophage polarization and osteoimmunomodulation.Graphical Abstract: Keywords: bredigite, three-dimensional printing, osteoimmunomodulation, macrophage polarization, tissue engineering |
| first_indexed | 2024-04-10T08:54:10Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-04-10T08:54:10Z |
| publishDate | 2023-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-1cd288f125da4e10a3fb4ded9538f7922023-02-21T18:00:13ZengDove Medical PressInternational Journal of Nanomedicine1178-20132023-02-01Volume 1891793281763The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage PolarizationXuan YLi LZhang CZhang MCao JZhang ZYaowei Xuan,1,* Lin Li,2,* Chenping Zhang,3,4 Min Zhang,1 Junkai Cao,1 Zhen Zhang3,4 1Department of Stomatology, The First Medical Centre, Chinese PLA General Hospital, Beijing, People’s Republic of China; 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center for Oral Diseases, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China; 3Department of Oral Maxillofacial-Head Neck Oncology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 4College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhen Zhang; Junkai Cao, Tel/Fax +86 153-1779-7335 ; +86 139-0129-0992, Email zz_nirvana@163.com; caojk301@163.comBackground: Repairing critical-sized bone defects secondary to traumatic or tumorous damage is a complex conundrum in clinical practice; in this case, artificial scaffolds exhibited preferable outcomes. Bredigite (BRT, Ca7MgSi4O16) bioceramic possesses excellent physicochemical properties and biological activity as a promising candidate for bone tissue engineering.Methods: Structurally ordered BRT (BRT-O) scaffolds were fabricated by a three-dimensional (3D) printing technique, and the random BRT (BRT-R) scaffolds and clinically available β-tricalcium phosphate (β-TCP) scaffolds were compared as control groups. Their physicochemical properties were characterized, and RAW 264.7 cells, bone marrow mesenchymal stem cells (BMSCs), and rat cranial critical-sized bone defect models were utilized for evaluating macrophage polarization and bone regeneration.Results: The BRT-O scaffolds exhibited regular morphology and homogeneous porosity. In addition, the BRT-O scaffolds released higher concentrations of ionic products based on coordinated biodegradability than the β-TCP scaffolds. In vitro, the BRT-O scaffolds facilitated RWA264.7 cells polarization to pro-healing M2 macrophage phenotype, whereas the BRT-R and β-TCP scaffolds stimulated more pro-inflammatory M1-type macrophages. A conditioned medium derived from macrophages seeding on the BRT-O scaffolds notably promoted the osteogenic lineage differentiation of BMSCs in vitro. The cell migration ability of BMSCs was significantly enhanced under the BRT-O-induced immune microenvironment. Moreover, in rat cranial critical-sized bone defect models, the BRT-O scaffolds group promoted new bone formation with a higher proportion of M2-type macrophage infiltration and expression of osteogenesis-related markers. Therefore, in vivo, BRT-O scaffolds play immunomodulatory roles in promoting critical-sized bone defects by enhancing the polarization of M2 macrophages.Conclusion: 3D-printed BRT-O scaffolds can be a promising option for bone tissue engineering, at least partly through macrophage polarization and osteoimmunomodulation.Graphical Abstract: Keywords: bredigite, three-dimensional printing, osteoimmunomodulation, macrophage polarization, tissue engineeringhttps://www.dovepress.com/the-3d-printed-ordered-bredigite-scaffold-promotes-pro-healing-of-crit-peer-reviewed-fulltext-article-IJNbredigitethree-dimensional printingosteoimmunomodulationmacrophage polarizationtissue engineering |
| spellingShingle | Xuan Y Li L Zhang C Zhang M Cao J Zhang Z The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage Polarization International Journal of Nanomedicine bredigite three-dimensional printing osteoimmunomodulation macrophage polarization tissue engineering |
| title | The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage Polarization |
| title_full | The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage Polarization |
| title_fullStr | The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage Polarization |
| title_full_unstemmed | The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage Polarization |
| title_short | The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage Polarization |
| title_sort | 3d printed ordered bredigite scaffold promotes pro healing of critical sized bone defects by regulating macrophage polarization |
| topic | bredigite three-dimensional printing osteoimmunomodulation macrophage polarization tissue engineering |
| url | https://www.dovepress.com/the-3d-printed-ordered-bredigite-scaffold-promotes-pro-healing-of-crit-peer-reviewed-fulltext-article-IJN |
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