The Impact of <i>Pseudomonas aeruginosa</i> Infection in Adult Cystic Fibrosis Patients—A Single Polish Centre Study
Background: <i>Pseudomonas aeruginosa</i> (PA) is one of the most predominant pathogens of lung infections, often causing exacerbations in adult patients with cystic fibrosis (CF). Materials and Methods: Microbiological characterization of 74 PA isolates and to evaluate the correlations...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/12/12/1440 |
| _version_ | 1827573827721232384 |
|---|---|
| author | Sylwia Jarzynka Oliwia Makarewicz Daniel Weiss Anna Minkiewicz-Zochniak Agnieszka Iwańska Wojciech Skorupa Marcin Padzik Ewa Augustynowicz-Kopeć Gabriela Olędzka |
| author_facet | Sylwia Jarzynka Oliwia Makarewicz Daniel Weiss Anna Minkiewicz-Zochniak Agnieszka Iwańska Wojciech Skorupa Marcin Padzik Ewa Augustynowicz-Kopeć Gabriela Olędzka |
| author_sort | Sylwia Jarzynka |
| collection | DOAJ |
| description | Background: <i>Pseudomonas aeruginosa</i> (PA) is one of the most predominant pathogens of lung infections, often causing exacerbations in adult patients with cystic fibrosis (CF). Materials and Methods: Microbiological characterization of 74 PA isolates and to evaluate the correlations between the bacterial features and 44 adult Polish CF cohort clinical parameters. Results: The most common variant in the CF transmembrane conductance regulator (<i>CFTR</i>) gene was F508del (76.3%), followed by 3849+10kbC>T (26.3%). A total of 39.4% of the PA isolates showed multiple resistances. In patients with parameters pointing to a decline in lung function, there was a statistically significant moderate correlation with β-lactam resistance and a weak correlation between hospital frequency and colistin resistance. The mucoidity did not correlate with the biofilm formation ability, which showed 41.9% of the isolates. Proteolytic activity, observed in 60.8% of the clinical isolates, was weakly associated with motility detected in 78.4% of the strains. The genetic profiles of the PA were highly heterogeneous, and a weak positive correlation was established between cluster group and biofilm formation. Conclusion: The findings suggest that there is a high variety in <i>P. aeruginosa</i> populations in adult CF patients. There is a need to monitor PA strains in groups of patients with cystic fibrosis, in particular, in terms of the occurrence of antibiotic resistance related to a decline in lung function. |
| first_indexed | 2024-03-08T20:26:42Z |
| format | Article |
| id | doaj.art-1cd326012f6e456bb120534269d45315 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-08T20:26:42Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-1cd326012f6e456bb120534269d453152023-12-22T14:31:00ZengMDPI AGPathogens2076-08172023-12-011212144010.3390/pathogens12121440The Impact of <i>Pseudomonas aeruginosa</i> Infection in Adult Cystic Fibrosis Patients—A Single Polish Centre StudySylwia Jarzynka0Oliwia Makarewicz1Daniel Weiss2Anna Minkiewicz-Zochniak3Agnieszka Iwańska4Wojciech Skorupa5Marcin Padzik6Ewa Augustynowicz-Kopeć7Gabriela Olędzka8Department of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575 Warsaw, PolandInstitute for Infectious Diseases and Infection Control, Jena University Hospital, 07747 Jena, GermanyInstitute for Infectious Diseases and Infection Control, Jena University Hospital, 07747 Jena, GermanyDepartment of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575 Warsaw, PolandDepartment of Microbiology, National Institute of Tuberculosis and Lung Diseases, Plocka 26, 01-138 Warsaw, PolandFirst Department of Lung Diseases, National Institute of Tuberculosis and Lung Diseases, Plocka 26, 01-138 Warsaw, PolandDepartment of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575 Warsaw, PolandDepartment of Microbiology, National Institute of Tuberculosis and Lung Diseases, Plocka 26, 01-138 Warsaw, PolandDepartment of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575 Warsaw, PolandBackground: <i>Pseudomonas aeruginosa</i> (PA) is one of the most predominant pathogens of lung infections, often causing exacerbations in adult patients with cystic fibrosis (CF). Materials and Methods: Microbiological characterization of 74 PA isolates and to evaluate the correlations between the bacterial features and 44 adult Polish CF cohort clinical parameters. Results: The most common variant in the CF transmembrane conductance regulator (<i>CFTR</i>) gene was F508del (76.3%), followed by 3849+10kbC>T (26.3%). A total of 39.4% of the PA isolates showed multiple resistances. In patients with parameters pointing to a decline in lung function, there was a statistically significant moderate correlation with β-lactam resistance and a weak correlation between hospital frequency and colistin resistance. The mucoidity did not correlate with the biofilm formation ability, which showed 41.9% of the isolates. Proteolytic activity, observed in 60.8% of the clinical isolates, was weakly associated with motility detected in 78.4% of the strains. The genetic profiles of the PA were highly heterogeneous, and a weak positive correlation was established between cluster group and biofilm formation. Conclusion: The findings suggest that there is a high variety in <i>P. aeruginosa</i> populations in adult CF patients. There is a need to monitor PA strains in groups of patients with cystic fibrosis, in particular, in terms of the occurrence of antibiotic resistance related to a decline in lung function.https://www.mdpi.com/2076-0817/12/12/1440cystic fibrosis<i>Pseudomonas aeruginosa</i>antimicrobial drug resistance |
| spellingShingle | Sylwia Jarzynka Oliwia Makarewicz Daniel Weiss Anna Minkiewicz-Zochniak Agnieszka Iwańska Wojciech Skorupa Marcin Padzik Ewa Augustynowicz-Kopeć Gabriela Olędzka The Impact of <i>Pseudomonas aeruginosa</i> Infection in Adult Cystic Fibrosis Patients—A Single Polish Centre Study Pathogens cystic fibrosis <i>Pseudomonas aeruginosa</i> antimicrobial drug resistance |
| title | The Impact of <i>Pseudomonas aeruginosa</i> Infection in Adult Cystic Fibrosis Patients—A Single Polish Centre Study |
| title_full | The Impact of <i>Pseudomonas aeruginosa</i> Infection in Adult Cystic Fibrosis Patients—A Single Polish Centre Study |
| title_fullStr | The Impact of <i>Pseudomonas aeruginosa</i> Infection in Adult Cystic Fibrosis Patients—A Single Polish Centre Study |
| title_full_unstemmed | The Impact of <i>Pseudomonas aeruginosa</i> Infection in Adult Cystic Fibrosis Patients—A Single Polish Centre Study |
| title_short | The Impact of <i>Pseudomonas aeruginosa</i> Infection in Adult Cystic Fibrosis Patients—A Single Polish Centre Study |
| title_sort | impact of i pseudomonas aeruginosa i infection in adult cystic fibrosis patients a single polish centre study |
| topic | cystic fibrosis <i>Pseudomonas aeruginosa</i> antimicrobial drug resistance |
| url | https://www.mdpi.com/2076-0817/12/12/1440 |
| work_keys_str_mv | AT sylwiajarzynka theimpactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT oliwiamakarewicz theimpactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT danielweiss theimpactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT annaminkiewiczzochniak theimpactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT agnieszkaiwanska theimpactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT wojciechskorupa theimpactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT marcinpadzik theimpactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT ewaaugustynowiczkopec theimpactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT gabrielaoledzka theimpactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT sylwiajarzynka impactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT oliwiamakarewicz impactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT danielweiss impactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT annaminkiewiczzochniak impactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT agnieszkaiwanska impactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT wojciechskorupa impactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT marcinpadzik impactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT ewaaugustynowiczkopec impactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy AT gabrielaoledzka impactofipseudomonasaeruginosaiinfectioninadultcysticfibrosispatientsasinglepolishcentrestudy |