Vancomycin Resistance in Enterococcus faecium Strains Isolated at a University Hospital
Genus Enterococcus species are gram positive cocci, usually inhabitants of human and animal intestinal tracts, but can also be isolated from their environmental sources. They are able to survive a wide range of adverse environmental stress conditions, allowing them to colonize a wide range of niches...
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| Format: | Article |
| Language: | English |
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2011-08-01
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| Series: | Kasmera |
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| Online Access: | https://produccioncientificaluz.org/index.php/kasmera/article/view/4869 |
| _version_ | 1797717240070537216 |
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| author | Armindo José Perozo Mena Maribel Josefina Castellano González Messaria María Ginestre Pérez Gresleida Coromoto Rincón Villalobos |
| author_facet | Armindo José Perozo Mena Maribel Josefina Castellano González Messaria María Ginestre Pérez Gresleida Coromoto Rincón Villalobos |
| author_sort | Armindo José Perozo Mena |
| collection | DOAJ |
| description | Genus Enterococcus species are gram positive cocci, usually inhabitants of human and animal intestinal tracts, but can also be isolated from their environmental sources. They are able to survive a wide range of adverse environmental stress conditions, allowing them to colonize a wide range of niches, including hospital environments. Nosocomial pathogenicity of enterococci has emerged in recent years, as well as their increased resistance to glycopeptide antibiotics. This study attempts to determine the resistance to vancomycin of Enterococcus faecium strains isolated in a university hospital during the January-December period of 2009, as well as to characterize the genetic determinant responsible for its resistance. Methods to determine susceptibility to glycopeptides described by CLSI were used; additionally, the genetic elements responsible for resistance were identified using the polymerase chain reaction. Of the E. faecium strains studied, 48.81% were resistant to vancomycin, and PCR showed presence of the vanA gene that confers high levels of resistance to glycopeptides. Due to the large number of isolates resistant to vancomycin, the presence of a nosocomial outbreak produced by this microorganism was suspected; this epidemic strain possessing a vanA gene is possibly scattered in different services of the institution; therefore, epidemiological studies should be performed to determine the role this microorganism plays in the production of nosocomial infections in the institution under study. |
| first_indexed | 2024-03-12T08:33:32Z |
| format | Article |
| id | doaj.art-1cd6167d6b1c4534bbbcfec937fb67f3 |
| institution | Directory Open Access Journal |
| issn | 0075-5222 2477-9628 |
| language | English |
| last_indexed | 2024-03-12T08:33:32Z |
| publishDate | 2011-08-01 |
| publisher | Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
| record_format | Article |
| series | Kasmera |
| spelling | doaj.art-1cd6167d6b1c4534bbbcfec937fb67f32023-09-02T17:24:01ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282011-08-0139171724358Vancomycin Resistance in Enterococcus faecium Strains Isolated at a University HospitalArmindo José Perozo Mena0Maribel Josefina Castellano González1Messaria María Ginestre Pérez2Gresleida Coromoto Rincón Villalobos3Escuela de Bioanálisis. Facultad de Medicina. Universidad del Zulia. Centro de Referencia Bacteriológica. Servicio autónomo Hospital Universitario de Maracaibo.Cátedra de Bacteriología General. Escuela de Bioanálisis. Facultad de Medicina. Universidad del Zulia.Cátedra de Bacteriología General. Escuela de Bioanálisis. Facultad de Medicina. Universidad del Zulia.Cátedra de Bacteriología Clínica. Escuela de Bioanálisis. Facultad de Medicina. Universidad del Zulia.Genus Enterococcus species are gram positive cocci, usually inhabitants of human and animal intestinal tracts, but can also be isolated from their environmental sources. They are able to survive a wide range of adverse environmental stress conditions, allowing them to colonize a wide range of niches, including hospital environments. Nosocomial pathogenicity of enterococci has emerged in recent years, as well as their increased resistance to glycopeptide antibiotics. This study attempts to determine the resistance to vancomycin of Enterococcus faecium strains isolated in a university hospital during the January-December period of 2009, as well as to characterize the genetic determinant responsible for its resistance. Methods to determine susceptibility to glycopeptides described by CLSI were used; additionally, the genetic elements responsible for resistance were identified using the polymerase chain reaction. Of the E. faecium strains studied, 48.81% were resistant to vancomycin, and PCR showed presence of the vanA gene that confers high levels of resistance to glycopeptides. Due to the large number of isolates resistant to vancomycin, the presence of a nosocomial outbreak produced by this microorganism was suspected; this epidemic strain possessing a vanA gene is possibly scattered in different services of the institution; therefore, epidemiological studies should be performed to determine the role this microorganism plays in the production of nosocomial infections in the institution under study.https://produccioncientificaluz.org/index.php/kasmera/article/view/4869enterococcus faeciumvancomicinaresistenciavanA |
| spellingShingle | Armindo José Perozo Mena Maribel Josefina Castellano González Messaria María Ginestre Pérez Gresleida Coromoto Rincón Villalobos Vancomycin Resistance in Enterococcus faecium Strains Isolated at a University Hospital Kasmera enterococcus faecium vancomicina resistencia vanA |
| title | Vancomycin Resistance in Enterococcus faecium Strains Isolated at a University Hospital |
| title_full | Vancomycin Resistance in Enterococcus faecium Strains Isolated at a University Hospital |
| title_fullStr | Vancomycin Resistance in Enterococcus faecium Strains Isolated at a University Hospital |
| title_full_unstemmed | Vancomycin Resistance in Enterococcus faecium Strains Isolated at a University Hospital |
| title_short | Vancomycin Resistance in Enterococcus faecium Strains Isolated at a University Hospital |
| title_sort | vancomycin resistance in enterococcus faecium strains isolated at a university hospital |
| topic | enterococcus faecium vancomicina resistencia vanA |
| url | https://produccioncientificaluz.org/index.php/kasmera/article/view/4869 |
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