Genetic dissection of grain architecture-related traits in a winter wheat population
Abstract Background The future productivity of wheat (T. aestivum L.) as the most grown crop worldwide is of utmost importance for global food security. Thousand kernel weight (TKW) in wheat is closely associated with grain architecture-related traits, e.g. kernel length (KL), kernel width (KW), ker...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-021-03183-3 |
| _version_ | 1819098758453919744 |
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| author | Matías Schierenbeck Ahmad M. Alqudah Ulrike Lohwasser Rasha A. Tarawneh María Rosa Simón Andreas Börner |
| author_facet | Matías Schierenbeck Ahmad M. Alqudah Ulrike Lohwasser Rasha A. Tarawneh María Rosa Simón Andreas Börner |
| author_sort | Matías Schierenbeck |
| collection | DOAJ |
| description | Abstract Background The future productivity of wheat (T. aestivum L.) as the most grown crop worldwide is of utmost importance for global food security. Thousand kernel weight (TKW) in wheat is closely associated with grain architecture-related traits, e.g. kernel length (KL), kernel width (KW), kernel area (KA), kernel diameter ratio (KDR), and factor form density (FFD). Discovering the genetic architecture of natural variation in these traits, identifying QTL and candidate genes are the main aims of this study. Therefore, grain architecture-related traits in 261 worldwide winter accessions over three field-year experiments were evaluated. Results Genome-wide association analysis using 90K SNP array in FarmCPU model revealed several interesting genomic regions including 17 significant SNPs passing false discovery rate threshold and strongly associated with the studied traits. Four of associated SNPs were physically located inside candidate genes within LD interval e.g. BobWhite_c5872_589 (602,710,399 bp) found to be inside TraesCS6A01G383800 (602,699,767–602,711,726 bp). Further analysis reveals the four novel candidate genes potentially involved in more than one grain architecture-related traits with a pleiotropic effects e.g. TraesCS6A01G383800 gene on 6A encoding oxidoreductase activity was associated with TKW and KA. The allelic variation at the associated SNPs showed significant differences betweeen the accessions carying the wild and mutated alleles e.g. accessions carying C allele of BobWhite_c5872_589, TraesCS6A01G383800 had significantly higher TKW than the accessions carying T allele. Interestingly, these genes were highly expressed in the grain-tissues, demonstrating their pivotal role in controlling the grain architecture. Conclusions These results are valuable for identifying regions associated with kernel weight and dimensions and potentially help breeders in improving kernel weight and architecture-related traits in order to increase wheat yield potential and end-use quality. |
| first_indexed | 2024-12-22T00:36:04Z |
| format | Article |
| id | doaj.art-1cd83ba77b9540d18b82798a51953210 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-22T00:36:04Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-1cd83ba77b9540d18b82798a519532102022-12-21T18:44:48ZengBMCBMC Plant Biology1471-22292021-09-0121111410.1186/s12870-021-03183-3Genetic dissection of grain architecture-related traits in a winter wheat populationMatías Schierenbeck0Ahmad M. Alqudah1Ulrike Lohwasser2Rasha A. Tarawneh3María Rosa Simón4Andreas Börner5Genebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)Department of Agroecology, Aarhus University at FlakkebjergGenebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)Genebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)Cereals, Faculty of Agricultural Sciences and Forestry, National University of La PlataGenebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)Abstract Background The future productivity of wheat (T. aestivum L.) as the most grown crop worldwide is of utmost importance for global food security. Thousand kernel weight (TKW) in wheat is closely associated with grain architecture-related traits, e.g. kernel length (KL), kernel width (KW), kernel area (KA), kernel diameter ratio (KDR), and factor form density (FFD). Discovering the genetic architecture of natural variation in these traits, identifying QTL and candidate genes are the main aims of this study. Therefore, grain architecture-related traits in 261 worldwide winter accessions over three field-year experiments were evaluated. Results Genome-wide association analysis using 90K SNP array in FarmCPU model revealed several interesting genomic regions including 17 significant SNPs passing false discovery rate threshold and strongly associated with the studied traits. Four of associated SNPs were physically located inside candidate genes within LD interval e.g. BobWhite_c5872_589 (602,710,399 bp) found to be inside TraesCS6A01G383800 (602,699,767–602,711,726 bp). Further analysis reveals the four novel candidate genes potentially involved in more than one grain architecture-related traits with a pleiotropic effects e.g. TraesCS6A01G383800 gene on 6A encoding oxidoreductase activity was associated with TKW and KA. The allelic variation at the associated SNPs showed significant differences betweeen the accessions carying the wild and mutated alleles e.g. accessions carying C allele of BobWhite_c5872_589, TraesCS6A01G383800 had significantly higher TKW than the accessions carying T allele. Interestingly, these genes were highly expressed in the grain-tissues, demonstrating their pivotal role in controlling the grain architecture. Conclusions These results are valuable for identifying regions associated with kernel weight and dimensions and potentially help breeders in improving kernel weight and architecture-related traits in order to increase wheat yield potential and end-use quality.https://doi.org/10.1186/s12870-021-03183-3Thousand kernel weightWinter wheatGWASGrain architectureCandidate genes |
| spellingShingle | Matías Schierenbeck Ahmad M. Alqudah Ulrike Lohwasser Rasha A. Tarawneh María Rosa Simón Andreas Börner Genetic dissection of grain architecture-related traits in a winter wheat population BMC Plant Biology Thousand kernel weight Winter wheat GWAS Grain architecture Candidate genes |
| title | Genetic dissection of grain architecture-related traits in a winter wheat population |
| title_full | Genetic dissection of grain architecture-related traits in a winter wheat population |
| title_fullStr | Genetic dissection of grain architecture-related traits in a winter wheat population |
| title_full_unstemmed | Genetic dissection of grain architecture-related traits in a winter wheat population |
| title_short | Genetic dissection of grain architecture-related traits in a winter wheat population |
| title_sort | genetic dissection of grain architecture related traits in a winter wheat population |
| topic | Thousand kernel weight Winter wheat GWAS Grain architecture Candidate genes |
| url | https://doi.org/10.1186/s12870-021-03183-3 |
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