Anti-Restriction Protein, KlcAHS, Promotes Dissemination of Carbapenem Resistance
Carbapenemase-producing Klebsiella pneumoniae (KPC) has emerged and spread throughout the world. A retrospective analysis was performed on carbapenem-resistant K. pneumoniae isolated at our teaching hospital during the period 2009–2010, when the initial outbreak occurred. To determine the mechanism(...
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00150/full |
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| author | Xiaofei Jiang Yuan Lu Wei Liang Yingzhou Xie Wei Xiong Yu Tang Gang Li |
| author_facet | Xiaofei Jiang Yuan Lu Wei Liang Yingzhou Xie Wei Xiong Yu Tang Gang Li |
| author_sort | Xiaofei Jiang |
| collection | DOAJ |
| description | Carbapenemase-producing Klebsiella pneumoniae (KPC) has emerged and spread throughout the world. A retrospective analysis was performed on carbapenem-resistant K. pneumoniae isolated at our teaching hospital during the period 2009–2010, when the initial outbreak occurred. To determine the mechanism(s) that underlies the increased infectivity exhibited by KPC, Multilocus Sequence Typing (MLST) was conducted. A series of plasmids was also extracted, sequenced and analyzed. Concurrently, the complete sequences of blaKPC−2-harboring plasmids deposited in GenBank were summarized and aligned. The blaKPC−2 and KlcAHS genes in the carbapenem-resistant K. pneumoniae isolates were examined. E. coli strains, carrying different Type I Restriction and Modification (RM) systems, were selected to study the interaction between RM systems, anti-RM systems and horizontal gene transfer (HGT). The ST11 clone predominated among 102 carbapenem-resistant K. pneumoniae isolates, all harbored the blaKPC−2 gene; 98% contained the KlcAHS gene. KlcAHS was one of the core genes in the backbone region of most blaKPC−2 carrying plasmids. Type I RM systems in the host bacteria reduced the rate of pHS10842 plasmid transformation by 30- to 40-fold. Presence of the anti-restriction protein, KlcAHS, on the other hand, increased transformation efficiency by 3- to 6-fold. These results indicate that RM systems can significantly restrict HGT. In contrast, KlcAHS can disrupt the RM systems and promote HGT by transformation. These findings suggest that the anti-restriction protein, KlcAHS, represents a novel mechanism that facilitates the increased transfer of blaKPC-2 and KlcAHS-carrying plasmids among K. pneumoniae strains. |
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| publishDate | 2017-05-01 |
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| spelling | doaj.art-1cda25b8bd38469f9cc38f26e448b6cc2022-12-21T23:23:42ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882017-05-01710.3389/fcimb.2017.00150247447Anti-Restriction Protein, KlcAHS, Promotes Dissemination of Carbapenem ResistanceXiaofei Jiang0Yuan Lu1Wei Liang2Yingzhou Xie3Wei Xiong4Yu Tang5Gang Li6Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan UniversityShanghai, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan UniversityShanghai, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan UniversityShanghai, ChinaState Key Laboratory for Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiaotong UniversityShanghai, ChinaState Key Laboratory for Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiaotong UniversityShanghai, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan UniversityShanghai, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan UniversityShanghai, ChinaCarbapenemase-producing Klebsiella pneumoniae (KPC) has emerged and spread throughout the world. A retrospective analysis was performed on carbapenem-resistant K. pneumoniae isolated at our teaching hospital during the period 2009–2010, when the initial outbreak occurred. To determine the mechanism(s) that underlies the increased infectivity exhibited by KPC, Multilocus Sequence Typing (MLST) was conducted. A series of plasmids was also extracted, sequenced and analyzed. Concurrently, the complete sequences of blaKPC−2-harboring plasmids deposited in GenBank were summarized and aligned. The blaKPC−2 and KlcAHS genes in the carbapenem-resistant K. pneumoniae isolates were examined. E. coli strains, carrying different Type I Restriction and Modification (RM) systems, were selected to study the interaction between RM systems, anti-RM systems and horizontal gene transfer (HGT). The ST11 clone predominated among 102 carbapenem-resistant K. pneumoniae isolates, all harbored the blaKPC−2 gene; 98% contained the KlcAHS gene. KlcAHS was one of the core genes in the backbone region of most blaKPC−2 carrying plasmids. Type I RM systems in the host bacteria reduced the rate of pHS10842 plasmid transformation by 30- to 40-fold. Presence of the anti-restriction protein, KlcAHS, on the other hand, increased transformation efficiency by 3- to 6-fold. These results indicate that RM systems can significantly restrict HGT. In contrast, KlcAHS can disrupt the RM systems and promote HGT by transformation. These findings suggest that the anti-restriction protein, KlcAHS, represents a novel mechanism that facilitates the increased transfer of blaKPC-2 and KlcAHS-carrying plasmids among K. pneumoniae strains.http://journal.frontiersin.org/article/10.3389/fcimb.2017.00150/fullK. pneumoniaeKlcAHSType I RM systemshorizontal gene transfertransformation |
| spellingShingle | Xiaofei Jiang Yuan Lu Wei Liang Yingzhou Xie Wei Xiong Yu Tang Gang Li Anti-Restriction Protein, KlcAHS, Promotes Dissemination of Carbapenem Resistance Frontiers in Cellular and Infection Microbiology K. pneumoniae KlcAHS Type I RM systems horizontal gene transfer transformation |
| title | Anti-Restriction Protein, KlcAHS, Promotes Dissemination of Carbapenem Resistance |
| title_full | Anti-Restriction Protein, KlcAHS, Promotes Dissemination of Carbapenem Resistance |
| title_fullStr | Anti-Restriction Protein, KlcAHS, Promotes Dissemination of Carbapenem Resistance |
| title_full_unstemmed | Anti-Restriction Protein, KlcAHS, Promotes Dissemination of Carbapenem Resistance |
| title_short | Anti-Restriction Protein, KlcAHS, Promotes Dissemination of Carbapenem Resistance |
| title_sort | anti restriction protein klcahs promotes dissemination of carbapenem resistance |
| topic | K. pneumoniae KlcAHS Type I RM systems horizontal gene transfer transformation |
| url | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00150/full |
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