Evaluate the Effect of Dendrosomal Curcumin on Expression Level of HOTAIR Long Noncoding RNA in MCF-7 Breast Cancer Cell Line
Introduction: Breast cancer is one of the most common malignancies in women. Treatment with herbal drugs has been considered by researchers due to the lower side effects. Curcumin is a polyphenol extracted from turmeric with confirmed anti-cancer properties. Curcumin is water-insoluble with rapid me...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Shahid Sadoughi University of Medical Sciences
2023-09-01
|
| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/article-1-5815-en.pdf |
| _version_ | 1797688356623089664 |
|---|---|
| author | Maryam Mehrabi Sara Alemohammad Reza Dashtebozorgi Maryam Tahmasebi Birgani Mohammad Reza Hajjari Javad Mohammadi-Asl |
| author_facet | Maryam Mehrabi Sara Alemohammad Reza Dashtebozorgi Maryam Tahmasebi Birgani Mohammad Reza Hajjari Javad Mohammadi-Asl |
| author_sort | Maryam Mehrabi |
| collection | DOAJ |
| description | Introduction: Breast cancer is one of the most common malignancies in women. Treatment with herbal drugs has been considered by researchers due to the lower side effects. Curcumin is a polyphenol extracted from turmeric with confirmed anti-cancer properties. Curcumin is water-insoluble with rapid metabolism. Drug delivery using nano-carriers is suggested to overcome such limitations. The aim of this study was based on evaluating the effect of Dendrosomes curcumin on expression level of HOTAIR long noncoding RNA in MCF-7 breast cancer cell line.
Methods: This study was an applied basic research. Firstly, curcumin was loaded in dendrosomes and the entry of dendrosomal curcumin into cells was studied using fluorescent microscopy. Cell death was investigated using MTT assay and apoptosis detection kit. The expression of HOTAIR gene was measured using real-time PCR. Increased expression of this gene was reported in a wide range of tumors. The data were analyzed using GraphPad Prism V9.5 statistical software, using NOVA one-way statistical analysis and Student t-test; the results were reported as mean ± standard deviation.
Results: Dendrosomes increased the solubility of curcumin. The effective inhibitory doses of dendrosomal curcumin after 24 and 48h treatment were 25 and 20 micromolar, respectively. The percent of cells undergoing early apoptosis were 22.97±0.03 and 56.22±0.05, respectively, which was statistically significant in comparison with non-treated control cells. Following 24 and 48h treatment with 20 micromolar of dendrosomal curcumin, the HOTAIR gene expression was significantly decreased (P=0.001).
Conclusion: These findings suggest that dendrosomal curcumin may promote breast tumor cells toward programmed cell death by reducing HOTAIR gene expression. |
| first_indexed | 2024-03-12T01:29:58Z |
| format | Article |
| id | doaj.art-1ce3536dd76f4a10a89b0d9075f62e11 |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-03-12T01:29:58Z |
| publishDate | 2023-09-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-1ce3536dd76f4a10a89b0d9075f62e112023-09-12T04:42:15ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332023-09-0131667186731Evaluate the Effect of Dendrosomal Curcumin on Expression Level of HOTAIR Long Noncoding RNA in MCF-7 Breast Cancer Cell LineMaryam Mehrabi0Sara Alemohammad1Reza Dashtebozorgi2Maryam Tahmasebi Birgani3Mohammad Reza Hajjari4Javad Mohammadi-Asl5 Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Department of Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran. Department of Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Depatrment of Biology, Faculty of Sciences, Shahid Chamran University of Ahva, Ahvaz, Iran. Department of Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Introduction: Breast cancer is one of the most common malignancies in women. Treatment with herbal drugs has been considered by researchers due to the lower side effects. Curcumin is a polyphenol extracted from turmeric with confirmed anti-cancer properties. Curcumin is water-insoluble with rapid metabolism. Drug delivery using nano-carriers is suggested to overcome such limitations. The aim of this study was based on evaluating the effect of Dendrosomes curcumin on expression level of HOTAIR long noncoding RNA in MCF-7 breast cancer cell line. Methods: This study was an applied basic research. Firstly, curcumin was loaded in dendrosomes and the entry of dendrosomal curcumin into cells was studied using fluorescent microscopy. Cell death was investigated using MTT assay and apoptosis detection kit. The expression of HOTAIR gene was measured using real-time PCR. Increased expression of this gene was reported in a wide range of tumors. The data were analyzed using GraphPad Prism V9.5 statistical software, using NOVA one-way statistical analysis and Student t-test; the results were reported as mean ± standard deviation. Results: Dendrosomes increased the solubility of curcumin. The effective inhibitory doses of dendrosomal curcumin after 24 and 48h treatment were 25 and 20 micromolar, respectively. The percent of cells undergoing early apoptosis were 22.97±0.03 and 56.22±0.05, respectively, which was statistically significant in comparison with non-treated control cells. Following 24 and 48h treatment with 20 micromolar of dendrosomal curcumin, the HOTAIR gene expression was significantly decreased (P=0.001). Conclusion: These findings suggest that dendrosomal curcumin may promote breast tumor cells toward programmed cell death by reducing HOTAIR gene expression.http://jssu.ssu.ac.ir/article-1-5815-en.pdfcurcumindendrosomebreast cancerlong noncoding rnahotair |
| spellingShingle | Maryam Mehrabi Sara Alemohammad Reza Dashtebozorgi Maryam Tahmasebi Birgani Mohammad Reza Hajjari Javad Mohammadi-Asl Evaluate the Effect of Dendrosomal Curcumin on Expression Level of HOTAIR Long Noncoding RNA in MCF-7 Breast Cancer Cell Line Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd curcumin dendrosome breast cancer long noncoding rna hotair |
| title | Evaluate the Effect of Dendrosomal Curcumin on Expression Level of HOTAIR Long Noncoding RNA in MCF-7 Breast Cancer Cell Line |
| title_full | Evaluate the Effect of Dendrosomal Curcumin on Expression Level of HOTAIR Long Noncoding RNA in MCF-7 Breast Cancer Cell Line |
| title_fullStr | Evaluate the Effect of Dendrosomal Curcumin on Expression Level of HOTAIR Long Noncoding RNA in MCF-7 Breast Cancer Cell Line |
| title_full_unstemmed | Evaluate the Effect of Dendrosomal Curcumin on Expression Level of HOTAIR Long Noncoding RNA in MCF-7 Breast Cancer Cell Line |
| title_short | Evaluate the Effect of Dendrosomal Curcumin on Expression Level of HOTAIR Long Noncoding RNA in MCF-7 Breast Cancer Cell Line |
| title_sort | evaluate the effect of dendrosomal curcumin on expression level of hotair long noncoding rna in mcf 7 breast cancer cell line |
| topic | curcumin dendrosome breast cancer long noncoding rna hotair |
| url | http://jssu.ssu.ac.ir/article-1-5815-en.pdf |
| work_keys_str_mv | AT maryammehrabi evaluatetheeffectofdendrosomalcurcuminonexpressionlevelofhotairlongnoncodingrnainmcf7breastcancercellline AT saraalemohammad evaluatetheeffectofdendrosomalcurcuminonexpressionlevelofhotairlongnoncodingrnainmcf7breastcancercellline AT rezadashtebozorgi evaluatetheeffectofdendrosomalcurcuminonexpressionlevelofhotairlongnoncodingrnainmcf7breastcancercellline AT maryamtahmasebibirgani evaluatetheeffectofdendrosomalcurcuminonexpressionlevelofhotairlongnoncodingrnainmcf7breastcancercellline AT mohammadrezahajjari evaluatetheeffectofdendrosomalcurcuminonexpressionlevelofhotairlongnoncodingrnainmcf7breastcancercellline AT javadmohammadiasl evaluatetheeffectofdendrosomalcurcuminonexpressionlevelofhotairlongnoncodingrnainmcf7breastcancercellline |