Antihyperglycemic activity of colostrum peptides
Peptides of plant and animal origin have good anti-diabetic prospects. The research objective was to use bovine colostrum peptides to reduce hyperglycemia in diabetic rats. Bovine colostrum peptides were obtained by trypsin hydrolysis of colostrum proteins with preliminary extraction of triglyceri...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kemerovo State University
2024-01-01
|
| Series: | Foods and Raw Materials |
| Subjects: | |
| Online Access: | https://jfrm.ru/en/issues/21683/21686/ |
| _version_ | 1797301638487080960 |
|---|---|
| author | Sergei L. Tikhonov Natalia V. Tikhonova Irina F. Gette Ksenia V. Sokolova Irina G. Danilova |
| author_facet | Sergei L. Tikhonov Natalia V. Tikhonova Irina F. Gette Ksenia V. Sokolova Irina G. Danilova |
| author_sort | Sergei L. Tikhonov |
| collection | DOAJ |
| description | Peptides of plant and animal origin have good anti-diabetic prospects. The research objective was to use bovine colostrum
peptides to reduce hyperglycemia in diabetic rats.
Bovine colostrum peptides were obtained by trypsin hydrolysis of colostrum proteins with preliminary extraction of triglycerides. The study involved four groups of Wistar rats with seven animals per group. Group 1 served as control; group 2 received
300 mg/kg of trypsin hydrolysate of bovine colostrum as part of their daily diet for 30 days. Groups 3 and 4 had diabetes mellitus
caused by intraperitoneal injections of 110 mg/kg of nicotinamide and 65 mg/kg of streptozotocin. Group 4 also received 300 mg/kg
trypsin hydrolysate of bovine colostrum intragastrically five times a week for 30 days.
Three peptides were isolated from the trypsin hydrolysate of bovine colostrum and tested for the sequence of amino acids and
molecular weight. Their identification involved the Protein NCBI database, followed by 2D and 3D modeling, which revealed
their chemical profile, pharmacological properties, and antioxidant activity. The diabetic rats treated with colostrum peptides
had lower glucose, glycated hemoglobin, malondialdehyde, and catalase activity but a higher content of glutathione in the blood.
Their leukocytes and erythrocytes also demonstrated less deviation from the standard. The antioxidant effect of colostrum protein hydrolysate depended on a peptide with the amino acid sequence of SQKKKNCPNGTRIRVPGPGP and a mass of 8.4 kDa.
Colostrum peptides reduced hyperglycemia and oxidative stress in diabetic rats. The research revealed good prospects for
isolating individual colostrum peptides to be tested for antidiabetic properties. |
| first_indexed | 2024-03-07T23:25:26Z |
| format | Article |
| id | doaj.art-1ce49f4ec92442548ddeec09de1b18b2 |
| institution | Directory Open Access Journal |
| issn | 2308-4057 2310-9599 |
| language | English |
| last_indexed | 2024-03-07T23:25:26Z |
| publishDate | 2024-01-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Foods and Raw Materials |
| spelling | doaj.art-1ce49f4ec92442548ddeec09de1b18b22024-02-21T04:25:06ZengKemerovo State UniversityFoods and Raw Materials2308-40572310-95992024-01-0112112413210.21603/2308-4057-2024-1-586Antihyperglycemic activity of colostrum peptidesSergei L. Tikhonov0https://orcid.org/0000-0003-4863-9834Natalia V. Tikhonova1https://orcid.org/0000-0001-5841-1791Irina F. Gette2https://orcid.org/0000-0003-3012-850XKsenia V. Sokolova3https://orcid.org/0000-0002-7024-4110Irina G. Danilova4https://orcid.org/0000-0001-6841-1197Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, Moscow, RussiaUral State Agrarian University, Yekaterinburg, RussiaInstitute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg, RussiaInstitute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg, RussiaInstitute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg, RussiaPeptides of plant and animal origin have good anti-diabetic prospects. The research objective was to use bovine colostrum peptides to reduce hyperglycemia in diabetic rats. Bovine colostrum peptides were obtained by trypsin hydrolysis of colostrum proteins with preliminary extraction of triglycerides. The study involved four groups of Wistar rats with seven animals per group. Group 1 served as control; group 2 received 300 mg/kg of trypsin hydrolysate of bovine colostrum as part of their daily diet for 30 days. Groups 3 and 4 had diabetes mellitus caused by intraperitoneal injections of 110 mg/kg of nicotinamide and 65 mg/kg of streptozotocin. Group 4 also received 300 mg/kg trypsin hydrolysate of bovine colostrum intragastrically five times a week for 30 days. Three peptides were isolated from the trypsin hydrolysate of bovine colostrum and tested for the sequence of amino acids and molecular weight. Their identification involved the Protein NCBI database, followed by 2D and 3D modeling, which revealed their chemical profile, pharmacological properties, and antioxidant activity. The diabetic rats treated with colostrum peptides had lower glucose, glycated hemoglobin, malondialdehyde, and catalase activity but a higher content of glutathione in the blood. Their leukocytes and erythrocytes also demonstrated less deviation from the standard. The antioxidant effect of colostrum protein hydrolysate depended on a peptide with the amino acid sequence of SQKKKNCPNGTRIRVPGPGP and a mass of 8.4 kDa. Colostrum peptides reduced hyperglycemia and oxidative stress in diabetic rats. The research revealed good prospects for isolating individual colostrum peptides to be tested for antidiabetic properties. https://jfrm.ru/en/issues/21683/21686/peptidesbovine colostrumdiabetes mellitusglucoseglycated hemoglobinantioxidant activity |
| spellingShingle | Sergei L. Tikhonov Natalia V. Tikhonova Irina F. Gette Ksenia V. Sokolova Irina G. Danilova Antihyperglycemic activity of colostrum peptides Foods and Raw Materials peptides bovine colostrum diabetes mellitus glucose glycated hemoglobin antioxidant activity |
| title | Antihyperglycemic activity of colostrum peptides |
| title_full | Antihyperglycemic activity of colostrum peptides |
| title_fullStr | Antihyperglycemic activity of colostrum peptides |
| title_full_unstemmed | Antihyperglycemic activity of colostrum peptides |
| title_short | Antihyperglycemic activity of colostrum peptides |
| title_sort | antihyperglycemic activity of colostrum peptides |
| topic | peptides bovine colostrum diabetes mellitus glucose glycated hemoglobin antioxidant activity |
| url | https://jfrm.ru/en/issues/21683/21686/ |
| work_keys_str_mv | AT sergeiltikhonov antihyperglycemicactivityofcolostrumpeptides AT nataliavtikhonova antihyperglycemicactivityofcolostrumpeptides AT irinafgette antihyperglycemicactivityofcolostrumpeptides AT kseniavsokolova antihyperglycemicactivityofcolostrumpeptides AT irinagdanilova antihyperglycemicactivityofcolostrumpeptides |