Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts
Gliding motility allows malaria parasites to migrate and invade tissues and cells in different hosts. It requires parasite surface proteins to provide attachment to host cells and extracellular matrices. Here, we identify the Plasmodium protein LIMP (the name refers to a gliding phenotype in the spo...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-05-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/24109 |
| _version_ | 1811181015756963840 |
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| author | Jorge M Santos Saskia Egarter Vanessa Zuzarte-Luís Hirdesh Kumar Catherine A Moreau Jessica Kehrer Andreia Pinto Mário da Costa Blandine Franke-Fayard Chris J Janse Friedrich Frischknecht Gunnar R Mair |
| author_facet | Jorge M Santos Saskia Egarter Vanessa Zuzarte-Luís Hirdesh Kumar Catherine A Moreau Jessica Kehrer Andreia Pinto Mário da Costa Blandine Franke-Fayard Chris J Janse Friedrich Frischknecht Gunnar R Mair |
| author_sort | Jorge M Santos |
| collection | DOAJ |
| description | Gliding motility allows malaria parasites to migrate and invade tissues and cells in different hosts. It requires parasite surface proteins to provide attachment to host cells and extracellular matrices. Here, we identify the Plasmodium protein LIMP (the name refers to a gliding phenotype in the sporozoite arising from epitope tagging of the endogenous protein) as a key regulator for adhesion during gliding motility in the rodent malaria model P. berghei. Transcribed in gametocytes, LIMP is translated in the ookinete from maternal mRNA, and later in the sporozoite. The absence of LIMP reduces initial mosquito infection by 50%, impedes salivary gland invasion 10-fold, and causes a complete absence of liver invasion as mutants fail to attach to host cells. GFP tagging of LIMP caused a limping defect during movement with reduced speed and transient curvature changes of the parasite. LIMP is an essential motility and invasion factor necessary for malaria transmission. |
| first_indexed | 2024-04-11T09:12:22Z |
| format | Article |
| id | doaj.art-1ce5f21225ae46859a5fc1b66697078b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:12:22Z |
| publishDate | 2017-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-1ce5f21225ae46859a5fc1b66697078b2022-12-22T04:32:28ZengeLife Sciences Publications LtdeLife2050-084X2017-05-01610.7554/eLife.24109Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hostsJorge M Santos0https://orcid.org/0000-0002-4940-5207Saskia Egarter1Vanessa Zuzarte-Luís2Hirdesh Kumar3Catherine A Moreau4Jessica Kehrer5https://orcid.org/0000-0001-5084-3485Andreia Pinto6Mário da Costa7Blandine Franke-Fayard8https://orcid.org/0000-0001-6041-4182Chris J Janse9Friedrich Frischknecht10Gunnar R Mair11https://orcid.org/0000-0002-5187-3691Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Edifício Egas Moniz, Av. Prof. Egas Moniz, Lisbon, PortugalParasitology, Department of Infectious Diseases, University of Heidelberg Medical School, Heidelberg, GermanyInstituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Edifício Egas Moniz, Av. Prof. Egas Moniz, Lisbon, PortugalParasitology, Department of Infectious Diseases, University of Heidelberg Medical School, Heidelberg, Germany; Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, IndiaParasitology, Department of Infectious Diseases, University of Heidelberg Medical School, Heidelberg, GermanyParasitology, Department of Infectious Diseases, University of Heidelberg Medical School, Heidelberg, GermanyInstituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Edifício Egas Moniz, Av. Prof. Egas Moniz, Lisbon, PortugalInstituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Edifício Egas Moniz, Av. Prof. Egas Moniz, Lisbon, PortugalLeiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center, Leiden, The NetherlandsLeiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center, Leiden, The NetherlandsParasitology, Department of Infectious Diseases, University of Heidelberg Medical School, Heidelberg, GermanyInstituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Edifício Egas Moniz, Av. Prof. Egas Moniz, Lisbon, Portugal; Parasitology, Department of Infectious Diseases, University of Heidelberg Medical School, Heidelberg, GermanyGliding motility allows malaria parasites to migrate and invade tissues and cells in different hosts. It requires parasite surface proteins to provide attachment to host cells and extracellular matrices. Here, we identify the Plasmodium protein LIMP (the name refers to a gliding phenotype in the sporozoite arising from epitope tagging of the endogenous protein) as a key regulator for adhesion during gliding motility in the rodent malaria model P. berghei. Transcribed in gametocytes, LIMP is translated in the ookinete from maternal mRNA, and later in the sporozoite. The absence of LIMP reduces initial mosquito infection by 50%, impedes salivary gland invasion 10-fold, and causes a complete absence of liver invasion as mutants fail to attach to host cells. GFP tagging of LIMP caused a limping defect during movement with reduced speed and transient curvature changes of the parasite. LIMP is an essential motility and invasion factor necessary for malaria transmission.https://elifesciences.org/articles/24109malariatransmissiongliding motilityPlasmodium bergheicell adhesion |
| spellingShingle | Jorge M Santos Saskia Egarter Vanessa Zuzarte-Luís Hirdesh Kumar Catherine A Moreau Jessica Kehrer Andreia Pinto Mário da Costa Blandine Franke-Fayard Chris J Janse Friedrich Frischknecht Gunnar R Mair Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts eLife malaria transmission gliding motility Plasmodium berghei cell adhesion |
| title | Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts |
| title_full | Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts |
| title_fullStr | Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts |
| title_full_unstemmed | Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts |
| title_short | Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts |
| title_sort | malaria parasite limp protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts |
| topic | malaria transmission gliding motility Plasmodium berghei cell adhesion |
| url | https://elifesciences.org/articles/24109 |
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