The ABC Transporter Components HgdB and HgdC are Important for Glycolipid Layer Composition and Function of Heterocysts in Anabaena sp. PCC 7120
Anabaena sp. PCC 7120 is a filamentous cyanobacterium able to fix atmospheric nitrogen in semi-regularly spaced heterocysts. For correct heterocyst function, a special cell envelope consisting of a glycolipid layer and a polysaccharide layer is essential. We investigated the role of the genes hgdB a...
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MDPI AG
2018-07-01
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| author | Dmitry Shvarev Carolina N. Nishi Lars Wörmer Iris Maldener |
| author_facet | Dmitry Shvarev Carolina N. Nishi Lars Wörmer Iris Maldener |
| author_sort | Dmitry Shvarev |
| collection | DOAJ |
| description | Anabaena sp. PCC 7120 is a filamentous cyanobacterium able to fix atmospheric nitrogen in semi-regularly spaced heterocysts. For correct heterocyst function, a special cell envelope consisting of a glycolipid layer and a polysaccharide layer is essential. We investigated the role of the genes hgdB and hgdC, encoding domains of a putative ABC transporter, in heterocyst maturation. We investigated the subcellular localization of the fusion protein HgdC-GFP and followed the differential expression of the hgdB and hgdC genes during heterocyst maturation. Using a single recombination approach, we created a mutant in hgdB gene and studied its phenotype by microscopy and analytical chromatography. Although heterocysts are formed in the mutant, the structure of the glycolipid layer is aberrant and also contains an atypical ratio of the two major glycolipids. As shown by a pull-down assay, HgdB interacts with the outer membrane protein TolC, which indicates a function as a type 1 secretion system. We show that the hgdB-hgdC genes are essential for the creation of micro-oxic conditions by influencing the correct composition of the glycolipid layer for heterocyst function. Our observations confirm the significance of the hgdB-hgdC gene cluster and shed light on a novel mode of regulation of heterocyst envelope formation. |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-04-11T11:00:30Z |
| publishDate | 2018-07-01 |
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| record_format | Article |
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| spelling | doaj.art-1ce78f4e4eb94510a729d40ecb61b7ca2022-12-22T04:28:38ZengMDPI AGLife2075-17292018-07-01832610.3390/life8030026life8030026The ABC Transporter Components HgdB and HgdC are Important for Glycolipid Layer Composition and Function of Heterocysts in Anabaena sp. PCC 7120Dmitry Shvarev0Carolina N. Nishi1Lars Wörmer2Iris Maldener3Organismic Interactions, Interfaculty Institute of Microbiology and Infection Medicine, Eberhard Karls University of Tübingen, 72076 Tübingen, GermanyOrganismic Interactions, Interfaculty Institute of Microbiology and Infection Medicine, Eberhard Karls University of Tübingen, 72076 Tübingen, GermanyOrganic Geochemistry Group, MARUM—Center for Marine Environmental Sciences, University of Bremen, 28359 Bremen, GermanyOrganismic Interactions, Interfaculty Institute of Microbiology and Infection Medicine, Eberhard Karls University of Tübingen, 72076 Tübingen, GermanyAnabaena sp. PCC 7120 is a filamentous cyanobacterium able to fix atmospheric nitrogen in semi-regularly spaced heterocysts. For correct heterocyst function, a special cell envelope consisting of a glycolipid layer and a polysaccharide layer is essential. We investigated the role of the genes hgdB and hgdC, encoding domains of a putative ABC transporter, in heterocyst maturation. We investigated the subcellular localization of the fusion protein HgdC-GFP and followed the differential expression of the hgdB and hgdC genes during heterocyst maturation. Using a single recombination approach, we created a mutant in hgdB gene and studied its phenotype by microscopy and analytical chromatography. Although heterocysts are formed in the mutant, the structure of the glycolipid layer is aberrant and also contains an atypical ratio of the two major glycolipids. As shown by a pull-down assay, HgdB interacts with the outer membrane protein TolC, which indicates a function as a type 1 secretion system. We show that the hgdB-hgdC genes are essential for the creation of micro-oxic conditions by influencing the correct composition of the glycolipid layer for heterocyst function. Our observations confirm the significance of the hgdB-hgdC gene cluster and shed light on a novel mode of regulation of heterocyst envelope formation.http://www.mdpi.com/2075-1729/8/3/26cyanobacteriaAnabaenaheterocystglycolipidABC transporterhgdBnitrogen fixation |
| spellingShingle | Dmitry Shvarev Carolina N. Nishi Lars Wörmer Iris Maldener The ABC Transporter Components HgdB and HgdC are Important for Glycolipid Layer Composition and Function of Heterocysts in Anabaena sp. PCC 7120 Life cyanobacteria Anabaena heterocyst glycolipid ABC transporter hgdB nitrogen fixation |
| title | The ABC Transporter Components HgdB and HgdC are Important for Glycolipid Layer Composition and Function of Heterocysts in Anabaena sp. PCC 7120 |
| title_full | The ABC Transporter Components HgdB and HgdC are Important for Glycolipid Layer Composition and Function of Heterocysts in Anabaena sp. PCC 7120 |
| title_fullStr | The ABC Transporter Components HgdB and HgdC are Important for Glycolipid Layer Composition and Function of Heterocysts in Anabaena sp. PCC 7120 |
| title_full_unstemmed | The ABC Transporter Components HgdB and HgdC are Important for Glycolipid Layer Composition and Function of Heterocysts in Anabaena sp. PCC 7120 |
| title_short | The ABC Transporter Components HgdB and HgdC are Important for Glycolipid Layer Composition and Function of Heterocysts in Anabaena sp. PCC 7120 |
| title_sort | abc transporter components hgdb and hgdc are important for glycolipid layer composition and function of heterocysts in anabaena sp pcc 7120 |
| topic | cyanobacteria Anabaena heterocyst glycolipid ABC transporter hgdB nitrogen fixation |
| url | http://www.mdpi.com/2075-1729/8/3/26 |
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