Stenotrophomonas goyi sp. nov., a novel bacterium associated with the alga Chlamydomonas reinhardtii [version 3; peer review: 2 approved]
Background A culture of the green algae Chlamydomonas reinhardtii was accidentally contaminated with three different bacteria in our laboratory facilities. This contaminated alga culture showed increased algal biohydrogen production. These three bacteria were independently isolated. Methods The chro...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2023-12-01
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| Series: | F1000Research |
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| Online Access: | https://f1000research.com/articles/12-1373/v3 |
| _version_ | 1797390662515032064 |
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| author | María Jesus Torres David González-Ballester Neda Fakhimi Alexandra Dubini |
| author_facet | María Jesus Torres David González-Ballester Neda Fakhimi Alexandra Dubini |
| author_sort | María Jesus Torres |
| collection | DOAJ |
| description | Background A culture of the green algae Chlamydomonas reinhardtii was accidentally contaminated with three different bacteria in our laboratory facilities. This contaminated alga culture showed increased algal biohydrogen production. These three bacteria were independently isolated. Methods The chromosomic DNA of one of the isolated bacteria was extracted and sequenced using PacBio technology. Tentative genome annotation (RAST server) and phylogenetic trees analysis (TYGS server) were conducted. Diverse growth tests were assayed for the bacterium and for the alga-bacterium consortium. Results Phylogenetic analysis indicates that the bacterium is a novel member of the Stenotrophomonas genus that has been termed in this work as S. goyi sp. nov. A fully sequenced genome (4,487,389 base pairs) and its tentative annotation (4,147 genes) are provided. The genome information suggests that S. goyi sp. nov. is unable to use sulfate and nitrate as sulfur and nitrogen sources, respectively. Growth tests have confirmed the dependence on the sulfur-containing amino acids methionine and cysteine. S. goyi sp. nov. and Chlamydomonas reinhardtii can establish a mutualistic relationship when cocultured together. Conclusions S. goyi sp. nov. could be of interest for the design of biotechnological approaches based on the use of artificial microalgae-bacteria multispecies consortia that take advantage of the complementary metabolic capacities of their different microorganisms. |
| first_indexed | 2024-03-08T23:21:29Z |
| format | Article |
| id | doaj.art-1ce83a6b518d4eb08a00c303a3d7d94e |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-03-08T23:21:29Z |
| publishDate | 2023-12-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-1ce83a6b518d4eb08a00c303a3d7d94e2023-12-15T01:00:01ZengF1000 Research LtdF1000Research2046-14022023-12-0112159432Stenotrophomonas goyi sp. nov., a novel bacterium associated with the alga Chlamydomonas reinhardtii [version 3; peer review: 2 approved]María Jesus Torres0https://orcid.org/0000-0003-0706-7833David González-Ballester1https://orcid.org/0000-0003-0024-1886Neda Fakhimi2https://orcid.org/0000-0002-9131-7737Alexandra Dubini3https://orcid.org/0000-0001-8825-3915Departamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales, Universidad de Cordoba, Córdoba, Andalusia, 14071, SpainDepartamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales, Universidad de Cordoba, Córdoba, Andalusia, 14071, SpainDepartamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales, Universidad de Cordoba, Córdoba, Andalusia, 14071, SpainDepartamento de Bioquímica y Biología Molecular, Campus Universitario de Rabanales, Universidad de Cordoba, Córdoba, Andalusia, 14071, SpainBackground A culture of the green algae Chlamydomonas reinhardtii was accidentally contaminated with three different bacteria in our laboratory facilities. This contaminated alga culture showed increased algal biohydrogen production. These three bacteria were independently isolated. Methods The chromosomic DNA of one of the isolated bacteria was extracted and sequenced using PacBio technology. Tentative genome annotation (RAST server) and phylogenetic trees analysis (TYGS server) were conducted. Diverse growth tests were assayed for the bacterium and for the alga-bacterium consortium. Results Phylogenetic analysis indicates that the bacterium is a novel member of the Stenotrophomonas genus that has been termed in this work as S. goyi sp. nov. A fully sequenced genome (4,487,389 base pairs) and its tentative annotation (4,147 genes) are provided. The genome information suggests that S. goyi sp. nov. is unable to use sulfate and nitrate as sulfur and nitrogen sources, respectively. Growth tests have confirmed the dependence on the sulfur-containing amino acids methionine and cysteine. S. goyi sp. nov. and Chlamydomonas reinhardtii can establish a mutualistic relationship when cocultured together. Conclusions S. goyi sp. nov. could be of interest for the design of biotechnological approaches based on the use of artificial microalgae-bacteria multispecies consortia that take advantage of the complementary metabolic capacities of their different microorganisms.https://f1000research.com/articles/12-1373/v3algae bacteria consortia cocultures Chlamydomonas Stenotrophomonaseng |
| spellingShingle | María Jesus Torres David González-Ballester Neda Fakhimi Alexandra Dubini Stenotrophomonas goyi sp. nov., a novel bacterium associated with the alga Chlamydomonas reinhardtii [version 3; peer review: 2 approved] F1000Research algae bacteria consortia cocultures Chlamydomonas Stenotrophomonas eng |
| title | Stenotrophomonas goyi sp. nov., a novel bacterium associated with the alga Chlamydomonas reinhardtii [version 3; peer review: 2 approved] |
| title_full | Stenotrophomonas goyi sp. nov., a novel bacterium associated with the alga Chlamydomonas reinhardtii [version 3; peer review: 2 approved] |
| title_fullStr | Stenotrophomonas goyi sp. nov., a novel bacterium associated with the alga Chlamydomonas reinhardtii [version 3; peer review: 2 approved] |
| title_full_unstemmed | Stenotrophomonas goyi sp. nov., a novel bacterium associated with the alga Chlamydomonas reinhardtii [version 3; peer review: 2 approved] |
| title_short | Stenotrophomonas goyi sp. nov., a novel bacterium associated with the alga Chlamydomonas reinhardtii [version 3; peer review: 2 approved] |
| title_sort | stenotrophomonas goyi sp nov a novel bacterium associated with the alga chlamydomonas reinhardtii version 3 peer review 2 approved |
| topic | algae bacteria consortia cocultures Chlamydomonas Stenotrophomonas eng |
| url | https://f1000research.com/articles/12-1373/v3 |
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