Extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids, Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae)
Abstract Animal mitochondrial genomes usually exhibit conserved gene arrangement across major lineages, while those in the Hymenoptera are known to possess frequent rearrangements, as are those of several other orders of insects. Here, we sequenced two complete mitochondrial genomes of Trichogramma...
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Nature Portfolio
2018-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-25338-3 |
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| author | Long Chen Peng-Yan Chen Xiao-Feng Xue Hai-Qing Hua Yuan-Xi Li Fan Zhang Shu-Jun Wei |
| author_facet | Long Chen Peng-Yan Chen Xiao-Feng Xue Hai-Qing Hua Yuan-Xi Li Fan Zhang Shu-Jun Wei |
| author_sort | Long Chen |
| collection | DOAJ |
| description | Abstract Animal mitochondrial genomes usually exhibit conserved gene arrangement across major lineages, while those in the Hymenoptera are known to possess frequent rearrangements, as are those of several other orders of insects. Here, we sequenced two complete mitochondrial genomes of Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae). In total, 37 mitochondrial genes were identified in both species. The same gene arrangement pattern was found in the two species, with extensive gene rearrangement compared with the ancestral insect mitochondrial genome. Most tRNA genes and all protein-coding genes were encoded on the minority strand. In total, 15 tRNA genes and seven protein-coding genes were rearranged. The rearrangements of cox1 and nad2 as well as most tRNA genes were novel. Phylogenetic analysis based on nucleotide sequences of protein-coding genes and on gene arrangement patterns produced identical topologies that support the relationship of (Agaonidae + Pteromalidae) + Trichogrammatidae in Chalcidoidea. CREx analysis revealed eight rearrangement operations occurred from presumed ancestral gene order of Chalcidoidea to form the derived gene order of Trichogramma. Our study shows that gene rearrangement information in Chalcidoidea can potentially contribute to the phylogeny of Chalcidoidea when more mitochondrial genome sequences are available. |
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| spelling | doaj.art-1ce99a643b4e44b984dddc4e62db17b32022-12-21T20:34:11ZengNature PortfolioScientific Reports2045-23222018-05-018111110.1038/s41598-018-25338-3Extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids, Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae)Long Chen0Peng-Yan Chen1Xiao-Feng Xue2Hai-Qing Hua3Yuan-Xi Li4Fan Zhang5Shu-Jun Wei6Department of Entomology, Nanjing Agricultural UniversityInstitute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry SciencesDepartment of Entomology, Nanjing Agricultural UniversityDepartment of Entomology, Nanjing Agricultural UniversityDepartment of Entomology, Nanjing Agricultural UniversityInstitute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry SciencesInstitute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry SciencesAbstract Animal mitochondrial genomes usually exhibit conserved gene arrangement across major lineages, while those in the Hymenoptera are known to possess frequent rearrangements, as are those of several other orders of insects. Here, we sequenced two complete mitochondrial genomes of Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae). In total, 37 mitochondrial genes were identified in both species. The same gene arrangement pattern was found in the two species, with extensive gene rearrangement compared with the ancestral insect mitochondrial genome. Most tRNA genes and all protein-coding genes were encoded on the minority strand. In total, 15 tRNA genes and seven protein-coding genes were rearranged. The rearrangements of cox1 and nad2 as well as most tRNA genes were novel. Phylogenetic analysis based on nucleotide sequences of protein-coding genes and on gene arrangement patterns produced identical topologies that support the relationship of (Agaonidae + Pteromalidae) + Trichogrammatidae in Chalcidoidea. CREx analysis revealed eight rearrangement operations occurred from presumed ancestral gene order of Chalcidoidea to form the derived gene order of Trichogramma. Our study shows that gene rearrangement information in Chalcidoidea can potentially contribute to the phylogeny of Chalcidoidea when more mitochondrial genome sequences are available.https://doi.org/10.1038/s41598-018-25338-3 |
| spellingShingle | Long Chen Peng-Yan Chen Xiao-Feng Xue Hai-Qing Hua Yuan-Xi Li Fan Zhang Shu-Jun Wei Extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids, Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae) Scientific Reports |
| title | Extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids, Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae) |
| title_full | Extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids, Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae) |
| title_fullStr | Extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids, Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae) |
| title_full_unstemmed | Extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids, Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae) |
| title_short | Extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids, Trichogramma japonicum and Trichogramma ostriniae (Hymenoptera: Chalcidoidea: Trichogrammatidae) |
| title_sort | extensive gene rearrangements in the mitochondrial genomes of two egg parasitoids trichogramma japonicum and trichogramma ostriniae hymenoptera chalcidoidea trichogrammatidae |
| url | https://doi.org/10.1038/s41598-018-25338-3 |
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