Chondrogenic Differentiation of Human Mesenchymal Stem Cells via SOX9 Delivery in Cationic Niosomes
Gene transfer to mesenchymal stem cells constitutes a powerful approach to promote their differentiation into the appropriate cartilage phenotype. Although viral vectors represent gold standard vehicles, because of their high efficiency, their use is precluded by important concerns including an elev...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/14/11/2327 |
| _version_ | 1797466793774678016 |
|---|---|
| author | Natalia Carballo-Pedrares Clara Sanjurjo-Rodriguez Jose Señarís Silvia Díaz-Prado Ana Rey-Rico |
| author_facet | Natalia Carballo-Pedrares Clara Sanjurjo-Rodriguez Jose Señarís Silvia Díaz-Prado Ana Rey-Rico |
| author_sort | Natalia Carballo-Pedrares |
| collection | DOAJ |
| description | Gene transfer to mesenchymal stem cells constitutes a powerful approach to promote their differentiation into the appropriate cartilage phenotype. Although viral vectors represent gold standard vehicles, because of their high efficiency, their use is precluded by important concerns including an elevated immunogenicity and the possibility of insertional mutagenesis. Therefore, the development of new and efficient non-viral vectors is under active investigation. In the present study, we developed new non-viral carriers based on niosomes to promote the effective chondrogenesis of human MSCs. Two different niosome formulations were prepared by varying their composition on non-ionic surfactant, polysorbate 80 solely (P80), or combined with poloxamer 407 (P80PX). The best niosome formulation was proven to transfer a plasmid, encoding for the potent chondrogenic transcription factor SOX9 in hMSC aggregate cultures. Transfection of hMSC aggregates via nioplexes resulted in an increased chondrogenic differentiation with reduced hypertrophy. These results highlight the potential of niosome formulations for gene therapy approaches focused on cartilage repair. |
| first_indexed | 2024-03-09T18:44:43Z |
| format | Article |
| id | doaj.art-1cea0cee6f7f4c189d58532e23611531 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T18:44:43Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-1cea0cee6f7f4c189d58532e236115312023-11-24T06:20:25ZengMDPI AGPharmaceutics1999-49232022-10-011411232710.3390/pharmaceutics14112327Chondrogenic Differentiation of Human Mesenchymal Stem Cells via SOX9 Delivery in Cationic NiosomesNatalia Carballo-Pedrares0Clara Sanjurjo-Rodriguez1Jose Señarís2Silvia Díaz-Prado3Ana Rey-Rico4Centro de Investigacións Científicas Avanzadas (CICA), Universidade da Coruña, As Carballeiras, s/n. Campus de Elviña, 15071 A Coruña, SpainCentro de Investigacións Científicas Avanzadas (CICA), Universidade da Coruña, As Carballeiras, s/n. Campus de Elviña, 15071 A Coruña, SpainInstitute of Biomedical Research of A Coruña (INIBIC), University Hospital Complex A Coruña (CHUAC), Galician Health Service (SERGAS), 15006 A Coruña, SpainCentro de Investigacións Científicas Avanzadas (CICA), Universidade da Coruña, As Carballeiras, s/n. Campus de Elviña, 15071 A Coruña, SpainCentro de Investigacións Científicas Avanzadas (CICA), Universidade da Coruña, As Carballeiras, s/n. Campus de Elviña, 15071 A Coruña, SpainGene transfer to mesenchymal stem cells constitutes a powerful approach to promote their differentiation into the appropriate cartilage phenotype. Although viral vectors represent gold standard vehicles, because of their high efficiency, their use is precluded by important concerns including an elevated immunogenicity and the possibility of insertional mutagenesis. Therefore, the development of new and efficient non-viral vectors is under active investigation. In the present study, we developed new non-viral carriers based on niosomes to promote the effective chondrogenesis of human MSCs. Two different niosome formulations were prepared by varying their composition on non-ionic surfactant, polysorbate 80 solely (P80), or combined with poloxamer 407 (P80PX). The best niosome formulation was proven to transfer a plasmid, encoding for the potent chondrogenic transcription factor SOX9 in hMSC aggregate cultures. Transfection of hMSC aggregates via nioplexes resulted in an increased chondrogenic differentiation with reduced hypertrophy. These results highlight the potential of niosome formulations for gene therapy approaches focused on cartilage repair.https://www.mdpi.com/1999-4923/14/11/2327niosomesnioplexeshuman mesenchymal stem cellsSOX9chondrogenesis |
| spellingShingle | Natalia Carballo-Pedrares Clara Sanjurjo-Rodriguez Jose Señarís Silvia Díaz-Prado Ana Rey-Rico Chondrogenic Differentiation of Human Mesenchymal Stem Cells via SOX9 Delivery in Cationic Niosomes Pharmaceutics niosomes nioplexes human mesenchymal stem cells SOX9 chondrogenesis |
| title | Chondrogenic Differentiation of Human Mesenchymal Stem Cells via SOX9 Delivery in Cationic Niosomes |
| title_full | Chondrogenic Differentiation of Human Mesenchymal Stem Cells via SOX9 Delivery in Cationic Niosomes |
| title_fullStr | Chondrogenic Differentiation of Human Mesenchymal Stem Cells via SOX9 Delivery in Cationic Niosomes |
| title_full_unstemmed | Chondrogenic Differentiation of Human Mesenchymal Stem Cells via SOX9 Delivery in Cationic Niosomes |
| title_short | Chondrogenic Differentiation of Human Mesenchymal Stem Cells via SOX9 Delivery in Cationic Niosomes |
| title_sort | chondrogenic differentiation of human mesenchymal stem cells via sox9 delivery in cationic niosomes |
| topic | niosomes nioplexes human mesenchymal stem cells SOX9 chondrogenesis |
| url | https://www.mdpi.com/1999-4923/14/11/2327 |
| work_keys_str_mv | AT nataliacarballopedrares chondrogenicdifferentiationofhumanmesenchymalstemcellsviasox9deliveryincationicniosomes AT clarasanjurjorodriguez chondrogenicdifferentiationofhumanmesenchymalstemcellsviasox9deliveryincationicniosomes AT josesenaris chondrogenicdifferentiationofhumanmesenchymalstemcellsviasox9deliveryincationicniosomes AT silviadiazprado chondrogenicdifferentiationofhumanmesenchymalstemcellsviasox9deliveryincationicniosomes AT anareyrico chondrogenicdifferentiationofhumanmesenchymalstemcellsviasox9deliveryincationicniosomes |