Evolution of Resistance to Chlorpyrifos and lambda-cyhalothrin Insecticides against Culex Pipiens Populations
The susceptibility of Culex pipiens larvae collected from Al-Asher of Ramadan, Sharkia Governorate, to chlorpyrifos and lambda-cyhalothrin insecticides was investigated for 20 successive generations. For multiple generations, the instar larvae of field parent strain were exposed to LC30 of the previ...
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| Format: | Article |
| Language: | English |
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Egyptian Society of Biological Sciences
2020-10-01
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| Series: | Egyptian Academic Journal of Biological Sciences. F, Toxicology and Pest Control |
| Subjects: | |
| Online Access: | https://eajbsf.journals.ekb.eg/article_122318_ce5eeec75088f0da904bdf6340c9d34f.pdf |
| _version_ | 1818645122436300800 |
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| author | Gharib, A. M. Mahmoud, M. M. El-Hassawy Fouad, A. F. Ali |
| author_facet | Gharib, A. M. Mahmoud, M. M. El-Hassawy Fouad, A. F. Ali |
| author_sort | Gharib, A. M. |
| collection | DOAJ |
| description | The susceptibility of Culex pipiens larvae collected from Al-Asher of Ramadan, Sharkia Governorate, to chlorpyrifos and lambda-cyhalothrin insecticides was investigated for 20 successive generations. For multiple generations, the instar larvae of field parent strain were exposed to LC30 of the previous generation to that insecticide. Total protein and lipids content as well as activities of detoxifying enzymes (i.e. acetylcholinesterase, non-specific esterases and glutathione-S-transferase) were determined in each generation. Bioassay tests showed that larval Cx. pipiens developed 144.31 and 761.85-fold resistance to chlorpyrifos and lambda-cyhalothrin, respectively, after 20 successive generations of selected pressure. Total protein content declined while total lipids increased gradually with proceeded the generations. In general, the activities of detoxifying enzymes increased gradually with raising generation numbers which indicate that the increased resistance is likely to be associated with the increased activity of target and metabolic enzyme systems. |
| first_indexed | 2024-12-17T00:25:43Z |
| format | Article |
| id | doaj.art-1cebdc6276974f56bd35fd053e2ac30b |
| institution | Directory Open Access Journal |
| issn | 2090-0864 2090-0791 |
| language | English |
| last_indexed | 2024-12-17T00:25:43Z |
| publishDate | 2020-10-01 |
| publisher | Egyptian Society of Biological Sciences |
| record_format | Article |
| series | Egyptian Academic Journal of Biological Sciences. F, Toxicology and Pest Control |
| spelling | doaj.art-1cebdc6276974f56bd35fd053e2ac30b2022-12-21T22:10:28ZengEgyptian Society of Biological SciencesEgyptian Academic Journal of Biological Sciences. F, Toxicology and Pest Control2090-08642090-07912020-10-01122189201DOI:10.21608/EAJBSF.2020.122318Evolution of Resistance to Chlorpyrifos and lambda-cyhalothrin Insecticides against Culex Pipiens PopulationsGharib, A. M.0 Mahmoud, M. M. El-Hassawy1 Fouad, A. F. Ali2Plant Protection Department, Faculty of Agriculture, Al-Azhar University, Cairo, EgyptPlant Protection Department, Faculty of Agriculture, Al-Azhar University, Cairo, EgyptPlant Protection Department, Faculty of Agriculture, Al-Azhar University, Cairo, EgyptThe susceptibility of Culex pipiens larvae collected from Al-Asher of Ramadan, Sharkia Governorate, to chlorpyrifos and lambda-cyhalothrin insecticides was investigated for 20 successive generations. For multiple generations, the instar larvae of field parent strain were exposed to LC30 of the previous generation to that insecticide. Total protein and lipids content as well as activities of detoxifying enzymes (i.e. acetylcholinesterase, non-specific esterases and glutathione-S-transferase) were determined in each generation. Bioassay tests showed that larval Cx. pipiens developed 144.31 and 761.85-fold resistance to chlorpyrifos and lambda-cyhalothrin, respectively, after 20 successive generations of selected pressure. Total protein content declined while total lipids increased gradually with proceeded the generations. In general, the activities of detoxifying enzymes increased gradually with raising generation numbers which indicate that the increased resistance is likely to be associated with the increased activity of target and metabolic enzyme systems.https://eajbsf.journals.ekb.eg/article_122318_ce5eeec75088f0da904bdf6340c9d34f.pdfculex pipiens; chemical insecticide; insecticides resistance; enzymes |
| spellingShingle | Gharib, A. M. Mahmoud, M. M. El-Hassawy Fouad, A. F. Ali Evolution of Resistance to Chlorpyrifos and lambda-cyhalothrin Insecticides against Culex Pipiens Populations Egyptian Academic Journal of Biological Sciences. F, Toxicology and Pest Control culex pipiens; chemical insecticide; insecticides resistance; enzymes |
| title | Evolution of Resistance to Chlorpyrifos and lambda-cyhalothrin Insecticides against Culex Pipiens Populations |
| title_full | Evolution of Resistance to Chlorpyrifos and lambda-cyhalothrin Insecticides against Culex Pipiens Populations |
| title_fullStr | Evolution of Resistance to Chlorpyrifos and lambda-cyhalothrin Insecticides against Culex Pipiens Populations |
| title_full_unstemmed | Evolution of Resistance to Chlorpyrifos and lambda-cyhalothrin Insecticides against Culex Pipiens Populations |
| title_short | Evolution of Resistance to Chlorpyrifos and lambda-cyhalothrin Insecticides against Culex Pipiens Populations |
| title_sort | evolution of resistance to chlorpyrifos and lambda cyhalothrin insecticides against culex pipiens populations |
| topic | culex pipiens; chemical insecticide; insecticides resistance; enzymes |
| url | https://eajbsf.journals.ekb.eg/article_122318_ce5eeec75088f0da904bdf6340c9d34f.pdf |
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