Corneal Healing and Recovery of Ocular Crystallinity with a Dichloromethane Extract of <i>Sedum dendroideum</i> D.C. in a Novel Murine Model of Ocular Pterygium
Pterygium is a corneal alteration that can cause visual impairment, which has been traditionally treated with the sap of <i>Sedum dendroideum</i> D.C. The pharmacological effect of a dichloromethane extract of S. dendroideum was demonstrated and implemented in a pterygium model on the he...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/15/4502 |
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| author | Luiselva Torrescano-De Labra Enrique Jiménez-Ferrer Brenda Hildeliza Camacho-Díaz Gabriela Vargas-Villa Manases González-Cortazar Maribel Herrera-Ruiz Sandra Victoria Ávila Reyes Javier Solorza-Feria Antonio Ruperto Jiménez-Aparicio |
| author_facet | Luiselva Torrescano-De Labra Enrique Jiménez-Ferrer Brenda Hildeliza Camacho-Díaz Gabriela Vargas-Villa Manases González-Cortazar Maribel Herrera-Ruiz Sandra Victoria Ávila Reyes Javier Solorza-Feria Antonio Ruperto Jiménez-Aparicio |
| author_sort | Luiselva Torrescano-De Labra |
| collection | DOAJ |
| description | Pterygium is a corneal alteration that can cause visual impairment, which has been traditionally treated with the sap of <i>Sedum dendroideum</i> D.C. The pharmacological effect of a dichloromethane extract of S. dendroideum was demonstrated and implemented in a pterygium model on the healing process of corneal damage caused by phorbol esters. In mice of the ICR strain, a corneal lesion was caused by intravitreal injection of tetradecanoylphorbol acetate (TPA). The evolution of the corneal scarring process was monitored with vehicle, dexamethasone, and dichloromethane extract of <i>S. dendroideum</i> treatments by daily ophthalmic administration for fifteen days. The lesions were evaluated in situ with highlighted images of fluorescence of the lesions. Following treatment levels in eyeballs of IL-1α, TNF-α, and IL-10 cytokines were measured. The effective dose of TPA to produce a pterygium-like lesion was determined. The follow-up of the evolution of the scarring process allowed us to define that the treatment with <i>S. dendroideum</i> improved the experimental pterygium and had an immunomodulatory effect by decreasing TNF-α, IL-1α, and maintaining the level of IL-10 expression, without difference with respect to the healthy control. Traditional medical use of <i>S. dendroideum</i> sap to treat pterygium is fully justified by its compound composition. |
| first_indexed | 2024-03-10T09:10:49Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T09:10:49Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1cedbadd14c84ad49dcf98330a3214902023-11-22T05:59:04ZengMDPI AGMolecules1420-30492021-07-012615450210.3390/molecules26154502Corneal Healing and Recovery of Ocular Crystallinity with a Dichloromethane Extract of <i>Sedum dendroideum</i> D.C. in a Novel Murine Model of Ocular PterygiumLuiselva Torrescano-De Labra0Enrique Jiménez-Ferrer1Brenda Hildeliza Camacho-Díaz2Gabriela Vargas-Villa3Manases González-Cortazar4Maribel Herrera-Ruiz5Sandra Victoria Ávila Reyes6Javier Solorza-Feria7Antonio Ruperto Jiménez-Aparicio8Centro de Investigación Biomédica del Sur (IMSS), Argentina No. 1, Col Centro, Xochitepec C.P. 62790, Morelos, MexicoCentro de Investigación Biomédica del Sur (IMSS), Argentina No. 1, Col Centro, Xochitepec C.P. 62790, Morelos, MexicoCentro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, Carretera Yautepec-Jojutla, Km. 6, Calle CEPROBI No. 8, Col. San Isidro, Yautepec C.P. 62731, Morelos, MexicoCentro de Investigación Biomédica del Sur (IMSS), Argentina No. 1, Col Centro, Xochitepec C.P. 62790, Morelos, MexicoCentro de Investigación Biomédica del Sur (IMSS), Argentina No. 1, Col Centro, Xochitepec C.P. 62790, Morelos, MexicoCentro de Investigación Biomédica del Sur (IMSS), Argentina No. 1, Col Centro, Xochitepec C.P. 62790, Morelos, MexicoCONACyT—Centro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, Carretera Yautepec-Jojutla, Km. 6, Calle CEPROBI, No. 8, Col. San Isidro, Yautepec C.P. 62731, Morelos, MexicoCentro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, Carretera Yautepec-Jojutla, Km. 6, Calle CEPROBI No. 8, Col. San Isidro, Yautepec C.P. 62731, Morelos, MexicoCentro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, Carretera Yautepec-Jojutla, Km. 6, Calle CEPROBI No. 8, Col. San Isidro, Yautepec C.P. 62731, Morelos, MexicoPterygium is a corneal alteration that can cause visual impairment, which has been traditionally treated with the sap of <i>Sedum dendroideum</i> D.C. The pharmacological effect of a dichloromethane extract of S. dendroideum was demonstrated and implemented in a pterygium model on the healing process of corneal damage caused by phorbol esters. In mice of the ICR strain, a corneal lesion was caused by intravitreal injection of tetradecanoylphorbol acetate (TPA). The evolution of the corneal scarring process was monitored with vehicle, dexamethasone, and dichloromethane extract of <i>S. dendroideum</i> treatments by daily ophthalmic administration for fifteen days. The lesions were evaluated in situ with highlighted images of fluorescence of the lesions. Following treatment levels in eyeballs of IL-1α, TNF-α, and IL-10 cytokines were measured. The effective dose of TPA to produce a pterygium-like lesion was determined. The follow-up of the evolution of the scarring process allowed us to define that the treatment with <i>S. dendroideum</i> improved the experimental pterygium and had an immunomodulatory effect by decreasing TNF-α, IL-1α, and maintaining the level of IL-10 expression, without difference with respect to the healthy control. Traditional medical use of <i>S. dendroideum</i> sap to treat pterygium is fully justified by its compound composition.https://www.mdpi.com/1420-3049/26/15/4502<i>Sedum dendroideum</i> D.C.pterygiumanti-inflammatoryscar process |
| spellingShingle | Luiselva Torrescano-De Labra Enrique Jiménez-Ferrer Brenda Hildeliza Camacho-Díaz Gabriela Vargas-Villa Manases González-Cortazar Maribel Herrera-Ruiz Sandra Victoria Ávila Reyes Javier Solorza-Feria Antonio Ruperto Jiménez-Aparicio Corneal Healing and Recovery of Ocular Crystallinity with a Dichloromethane Extract of <i>Sedum dendroideum</i> D.C. in a Novel Murine Model of Ocular Pterygium Molecules <i>Sedum dendroideum</i> D.C. pterygium anti-inflammatory scar process |
| title | Corneal Healing and Recovery of Ocular Crystallinity with a Dichloromethane Extract of <i>Sedum dendroideum</i> D.C. in a Novel Murine Model of Ocular Pterygium |
| title_full | Corneal Healing and Recovery of Ocular Crystallinity with a Dichloromethane Extract of <i>Sedum dendroideum</i> D.C. in a Novel Murine Model of Ocular Pterygium |
| title_fullStr | Corneal Healing and Recovery of Ocular Crystallinity with a Dichloromethane Extract of <i>Sedum dendroideum</i> D.C. in a Novel Murine Model of Ocular Pterygium |
| title_full_unstemmed | Corneal Healing and Recovery of Ocular Crystallinity with a Dichloromethane Extract of <i>Sedum dendroideum</i> D.C. in a Novel Murine Model of Ocular Pterygium |
| title_short | Corneal Healing and Recovery of Ocular Crystallinity with a Dichloromethane Extract of <i>Sedum dendroideum</i> D.C. in a Novel Murine Model of Ocular Pterygium |
| title_sort | corneal healing and recovery of ocular crystallinity with a dichloromethane extract of i sedum dendroideum i d c in a novel murine model of ocular pterygium |
| topic | <i>Sedum dendroideum</i> D.C. pterygium anti-inflammatory scar process |
| url | https://www.mdpi.com/1420-3049/26/15/4502 |
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