Association of significantly elevated plasma levels of NGAL and IGFBP4 in patients with diabetic nephropathy
Abstract Background Diabetic nephropathy (DN) is a type of progressive kidney disease affecting approximately 40% of patients with diabetes. Current DN diagnostic criteria predominantly rely on albuminuria and serum creatinine (sCr) levels. However, the specificity and reliability of both markers ar...
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| Format: | Article |
| Language: | English |
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BMC
2022-02-01
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| Series: | BMC Nephrology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12882-022-02692-z |
| _version_ | 1818977137595514880 |
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| author | Hamad Ali Mohamed Abu-Farha Eman Alshawaf Sriraman Devarajan Yousif Bahbahani Irina Al-Khairi Preethi Cherian Zahra Alsairafi Vidya Vijayan Fahd Al-Mulla Abdulnabi Al Attar Jehad Abubaker |
| author_facet | Hamad Ali Mohamed Abu-Farha Eman Alshawaf Sriraman Devarajan Yousif Bahbahani Irina Al-Khairi Preethi Cherian Zahra Alsairafi Vidya Vijayan Fahd Al-Mulla Abdulnabi Al Attar Jehad Abubaker |
| author_sort | Hamad Ali |
| collection | DOAJ |
| description | Abstract Background Diabetic nephropathy (DN) is a type of progressive kidney disease affecting approximately 40% of patients with diabetes. Current DN diagnostic criteria predominantly rely on albuminuria and serum creatinine (sCr) levels. However, the specificity and reliability of both markers are limited. Hence, reliable biomarkers are required for early diagnosis to effectively manage DN progression. Methods In this study, a cohort of 159 individuals were clinically evaluated and the plasma levels of NGAL, IGFBP-1, IGFBP-3, and IGFBP-4 were determined using Multiplexing Assays. Additionally, the association between the plasma levels of NGAL, IGFBP-1, IGFBP-3, and IGFBP-4 in patients with DN were compared to those in patients with T2D without kidney disease and control participants. Results Circulating level of NGAL were significantly higher in people with DN compared to people with T2D and non-diabetic groups (92.76 ± 7.5, 57.22 ± 8.7, and 52.47 ± 2.9 mg/L, respectively; p < 0.0001). IGFBP-4 showed a similar pattern, where it was highest in people with DN (795.61 ng/ml ±130.7) compared to T2D and non-diabetic people (374.56 ng/ml ±86.8, 273.06 ng/ml ±27.8 respectively, ANOVA p < 0.01). The data from this study shows a significant positive correlation between NGAL and IGFBP-4 in people with DN (ρ = .620, p < 0.005). IGFBP-4 also correlated positively with creatinine level and negatively with eGFR, in people with DN supporting its involvement in DN. Conclusion The data from this study shows a parallel increase in the plasma levels of NGAL and IGFBP-4 in DN. This highlights the potential to use these markers for early diagnosis of DN. |
| first_indexed | 2024-12-20T16:22:58Z |
| format | Article |
| id | doaj.art-1cf22411aa854c74b5e5df1f7ff3d5e7 |
| institution | Directory Open Access Journal |
| issn | 1471-2369 |
| language | English |
| last_indexed | 2024-12-20T16:22:58Z |
| publishDate | 2022-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Nephrology |
| spelling | doaj.art-1cf22411aa854c74b5e5df1f7ff3d5e72022-12-21T19:33:33ZengBMCBMC Nephrology1471-23692022-02-0123111010.1186/s12882-022-02692-zAssociation of significantly elevated plasma levels of NGAL and IGFBP4 in patients with diabetic nephropathyHamad Ali0Mohamed Abu-Farha1Eman Alshawaf2Sriraman Devarajan3Yousif Bahbahani4Irina Al-Khairi5Preethi Cherian6Zahra Alsairafi7Vidya Vijayan8Fahd Al-Mulla9Abdulnabi Al Attar10Jehad Abubaker11Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Health Sciences Center, Kuwait UniversityDepartment of Biochemistry and Molecular Biology, Dasman Diabetes Institute (DDI)Department of Biochemistry and Molecular Biology, Dasman Diabetes Institute (DDI)National Dasman Diabetes Biobank, Dasman Diabetes Institute (DDI)Medical Division, Dasman Diabetes Institute (DDI)Department of Biochemistry and Molecular Biology, Dasman Diabetes Institute (DDI)Department of Biochemistry and Molecular Biology, Dasman Diabetes Institute (DDI)Department of Pharmacy Practice, Faculty of Pharmacy, Health Sciences Center, Kuwait UniversityNational Dasman Diabetes Biobank, Dasman Diabetes Institute (DDI)Department of Genetics and Bioinformatics, Dasman Diabetes Institute (DDI)Medical Division, Dasman Diabetes Institute (DDI)Department of Biochemistry and Molecular Biology, Dasman Diabetes Institute (DDI)Abstract Background Diabetic nephropathy (DN) is a type of progressive kidney disease affecting approximately 40% of patients with diabetes. Current DN diagnostic criteria predominantly rely on albuminuria and serum creatinine (sCr) levels. However, the specificity and reliability of both markers are limited. Hence, reliable biomarkers are required for early diagnosis to effectively manage DN progression. Methods In this study, a cohort of 159 individuals were clinically evaluated and the plasma levels of NGAL, IGFBP-1, IGFBP-3, and IGFBP-4 were determined using Multiplexing Assays. Additionally, the association between the plasma levels of NGAL, IGFBP-1, IGFBP-3, and IGFBP-4 in patients with DN were compared to those in patients with T2D without kidney disease and control participants. Results Circulating level of NGAL were significantly higher in people with DN compared to people with T2D and non-diabetic groups (92.76 ± 7.5, 57.22 ± 8.7, and 52.47 ± 2.9 mg/L, respectively; p < 0.0001). IGFBP-4 showed a similar pattern, where it was highest in people with DN (795.61 ng/ml ±130.7) compared to T2D and non-diabetic people (374.56 ng/ml ±86.8, 273.06 ng/ml ±27.8 respectively, ANOVA p < 0.01). The data from this study shows a significant positive correlation between NGAL and IGFBP-4 in people with DN (ρ = .620, p < 0.005). IGFBP-4 also correlated positively with creatinine level and negatively with eGFR, in people with DN supporting its involvement in DN. Conclusion The data from this study shows a parallel increase in the plasma levels of NGAL and IGFBP-4 in DN. This highlights the potential to use these markers for early diagnosis of DN.https://doi.org/10.1186/s12882-022-02692-zDNNGALIGFBP-4IGFBP, diabetes |
| spellingShingle | Hamad Ali Mohamed Abu-Farha Eman Alshawaf Sriraman Devarajan Yousif Bahbahani Irina Al-Khairi Preethi Cherian Zahra Alsairafi Vidya Vijayan Fahd Al-Mulla Abdulnabi Al Attar Jehad Abubaker Association of significantly elevated plasma levels of NGAL and IGFBP4 in patients with diabetic nephropathy BMC Nephrology DN NGAL IGFBP-4 IGFBP, diabetes |
| title | Association of significantly elevated plasma levels of NGAL and IGFBP4 in patients with diabetic nephropathy |
| title_full | Association of significantly elevated plasma levels of NGAL and IGFBP4 in patients with diabetic nephropathy |
| title_fullStr | Association of significantly elevated plasma levels of NGAL and IGFBP4 in patients with diabetic nephropathy |
| title_full_unstemmed | Association of significantly elevated plasma levels of NGAL and IGFBP4 in patients with diabetic nephropathy |
| title_short | Association of significantly elevated plasma levels of NGAL and IGFBP4 in patients with diabetic nephropathy |
| title_sort | association of significantly elevated plasma levels of ngal and igfbp4 in patients with diabetic nephropathy |
| topic | DN NGAL IGFBP-4 IGFBP, diabetes |
| url | https://doi.org/10.1186/s12882-022-02692-z |
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