Anti-diabetic activity of extract from Morus nigra L. twigs through activation of AMPK/PKC pathway in mice
Morus nigra L. is a traditional ethnomedicinal plants, which is used for the treatment of atherosclerosis, diabetes, hyperlipidemia, and hypertension. The aim of this study was to investigate the antidiabetic activity and potential mechanisms of ethyl acetate extract of Morus nigra L. twigs (MNT-EA)...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622004285 |
| _version_ | 1811178910097866752 |
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| author | Ping Tang Xinzhou Yang Huijian Chen Ting Zhang Hui Tang Kejian Pang |
| author_facet | Ping Tang Xinzhou Yang Huijian Chen Ting Zhang Hui Tang Kejian Pang |
| author_sort | Ping Tang |
| collection | DOAJ |
| description | Morus nigra L. is a traditional ethnomedicinal plants, which is used for the treatment of atherosclerosis, diabetes, hyperlipidemia, and hypertension. The aim of this study was to investigate the antidiabetic activity and potential mechanisms of ethyl acetate extract of Morus nigra L. twigs (MNT-EA). In L6 cells, MNT-EA significantly upregulated glucose uptake, and promoted GLUT4 translocation and expression through AMPK and PKC signaling pathway. In vivo, diabetic mice treated with MNT-EA for 4 weeks improved hyperglycemia, lipid metabolism disorders and insulin resistance. According to histopathological examination, MNT-EA attenuated hepatic steatosis, adipocyte hypertrophy, and islet lesions in diabetic mice. In addition, MNT-EA upregulated p-AMPK, p-PKC, and GLUT4 expression in liver, adipose, and skeletal muscle tissues, thereby enhancing glucose uptake and improving diabetic symptoms. It is the first time to demonstrate that MNT-EA exerted anti-diabetic effects through activation of AMPK/PKC/GLUT4 signaling pathway, providing a basis for the development of new anti-diabetic drugs. |
| first_indexed | 2024-04-11T06:25:59Z |
| format | Article |
| id | doaj.art-1cf24d6b0592436182621ed8f1f30f3e |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-11T06:25:59Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-1cf24d6b0592436182621ed8f1f30f3e2022-12-22T04:40:21ZengElsevierJournal of Functional Foods1756-46462022-12-0199105358Anti-diabetic activity of extract from Morus nigra L. twigs through activation of AMPK/PKC pathway in micePing Tang0Xinzhou Yang1Huijian Chen2Ting Zhang3Hui Tang4Kejian Pang5Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, Pharmacy School of Shihezi University, Shihezi 832002, Xinjiang, PR ChinaSchool of Pharmaceutical Sciences, South-Central Minzu University, 182 Min-Zu Road, Wuhan, ChinaSchool of Pharmaceutical Sciences, South-Central Minzu University, 182 Min-Zu Road, Wuhan, ChinaSchool of Pharmaceutical Sciences, South-Central Minzu University, 182 Min-Zu Road, Wuhan, ChinaKey Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, Pharmacy School of Shihezi University, Shihezi 832002, Xinjiang, PR China; Corresponding authors at: North 2nd Road, Shihezi, China.Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, Pharmacy School of Shihezi University, Shihezi 832002, Xinjiang, PR China; Corresponding authors at: North 2nd Road, Shihezi, China.Morus nigra L. is a traditional ethnomedicinal plants, which is used for the treatment of atherosclerosis, diabetes, hyperlipidemia, and hypertension. The aim of this study was to investigate the antidiabetic activity and potential mechanisms of ethyl acetate extract of Morus nigra L. twigs (MNT-EA). In L6 cells, MNT-EA significantly upregulated glucose uptake, and promoted GLUT4 translocation and expression through AMPK and PKC signaling pathway. In vivo, diabetic mice treated with MNT-EA for 4 weeks improved hyperglycemia, lipid metabolism disorders and insulin resistance. According to histopathological examination, MNT-EA attenuated hepatic steatosis, adipocyte hypertrophy, and islet lesions in diabetic mice. In addition, MNT-EA upregulated p-AMPK, p-PKC, and GLUT4 expression in liver, adipose, and skeletal muscle tissues, thereby enhancing glucose uptake and improving diabetic symptoms. It is the first time to demonstrate that MNT-EA exerted anti-diabetic effects through activation of AMPK/PKC/GLUT4 signaling pathway, providing a basis for the development of new anti-diabetic drugs.http://www.sciencedirect.com/science/article/pii/S1756464622004285Morus nigra L.Type 2 diabetes mellitusGlucose transporter 4 (GLUT4)Protein kinase C (PKC)AMP-activated protein kinase (AMPK) |
| spellingShingle | Ping Tang Xinzhou Yang Huijian Chen Ting Zhang Hui Tang Kejian Pang Anti-diabetic activity of extract from Morus nigra L. twigs through activation of AMPK/PKC pathway in mice Journal of Functional Foods Morus nigra L. Type 2 diabetes mellitus Glucose transporter 4 (GLUT4) Protein kinase C (PKC) AMP-activated protein kinase (AMPK) |
| title | Anti-diabetic activity of extract from Morus nigra L. twigs through activation of AMPK/PKC pathway in mice |
| title_full | Anti-diabetic activity of extract from Morus nigra L. twigs through activation of AMPK/PKC pathway in mice |
| title_fullStr | Anti-diabetic activity of extract from Morus nigra L. twigs through activation of AMPK/PKC pathway in mice |
| title_full_unstemmed | Anti-diabetic activity of extract from Morus nigra L. twigs through activation of AMPK/PKC pathway in mice |
| title_short | Anti-diabetic activity of extract from Morus nigra L. twigs through activation of AMPK/PKC pathway in mice |
| title_sort | anti diabetic activity of extract from morus nigra l twigs through activation of ampk pkc pathway in mice |
| topic | Morus nigra L. Type 2 diabetes mellitus Glucose transporter 4 (GLUT4) Protein kinase C (PKC) AMP-activated protein kinase (AMPK) |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622004285 |
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