In vivo effects of different anesthetic agents on apoptosis
BackgroundThis study was designed to measure in vivo effects of propofol, isoflurane and sevoflurane on apoptosis by measuring caspase-3 and tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL) blood level as apoptotic markers.MethodsAfter obtaining ethical committee approval and in...
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| Format: | Article |
| Language: | English |
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Korean Society of Anesthesiologists
2012-07-01
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| Series: | Korean Journal of Anesthesiology |
| Subjects: | |
| Online Access: | http://ekja.org/upload/pdf/kjae-63-18.pdf |
| _version_ | 1819112989143334912 |
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| author | Emad S. Osman Hanan F. Khafagy Yasser M. Samhan Mona M. Hassan Faten M. El-Shanawany Abdel Rahman M. Fathallah Gehan G. El-fandy |
| author_facet | Emad S. Osman Hanan F. Khafagy Yasser M. Samhan Mona M. Hassan Faten M. El-Shanawany Abdel Rahman M. Fathallah Gehan G. El-fandy |
| author_sort | Emad S. Osman |
| collection | DOAJ |
| description | BackgroundThis study was designed to measure in vivo effects of propofol, isoflurane and sevoflurane on apoptosis by measuring caspase-3 and tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL) blood level as apoptotic markers.MethodsAfter obtaining ethical committee approval and informed written consents, sixty adult patients ASA I scheduled for open cholecystectomy participated in this study. They were randomally allocated into one of three equal groups to receive propofol infusion, low-flow isoflurane or sevoflurane for maintenance of anesthesia. Venous blood samples were collected preoperatively, immediately postoperative and after 24 hours to measure hemoglobin, hematocrit, creatinine, liver enzymes, serum TRAIL and caspase-3 levels.ResultsThere was no significant difference in hematological markers and serum creatinine. Liver enzymes showed significant postoperative rise (P < 0.05). In Propofol group, TRAIL and caspase-3 levels were significantly elevated immediately postoperative then decreased significantly after 24-hours (P < 0.05). In Isoflurane group, immediate postoperative level of TRAIL was significantly higher than 24 hours reading and significantly lower than its level in Propofol group at the same timing meanwhile caspase-3 levels were comparable at different timings. In Sevoflurane group, TRAIL and caspase-3 levels increased significantly in both postoperative samples than preoperative level and than those of Isoflurane and Propofol groups after 24 hours concerning TRAIL (P & 0.05).ConclusionsThis study concluded that isoflurane is superior and sevoflurane is the least effective among the three anesthetics in protection against apoptosis. This study neither proved nor excluded propofol-induced apoptosis. Further studies are required during lengthy procedure and in compromised patients. |
| first_indexed | 2024-12-22T04:22:16Z |
| format | Article |
| id | doaj.art-1cf3058d7efe446d8f24d1ef8ced85bb |
| institution | Directory Open Access Journal |
| issn | 2005-6419 2005-7563 |
| language | English |
| last_indexed | 2024-12-22T04:22:16Z |
| publishDate | 2012-07-01 |
| publisher | Korean Society of Anesthesiologists |
| record_format | Article |
| series | Korean Journal of Anesthesiology |
| spelling | doaj.art-1cf3058d7efe446d8f24d1ef8ced85bb2022-12-21T18:39:14ZengKorean Society of AnesthesiologistsKorean Journal of Anesthesiology2005-64192005-75632012-07-01631182410.4097/kjae.2012.63.1.187397In vivo effects of different anesthetic agents on apoptosisEmad S. Osman0Hanan F. Khafagy1Yasser M. Samhan2Mona M. Hassan3Faten M. El-Shanawany4Abdel Rahman M. Fathallah5Gehan G. El-fandy6Department of Anesthesiology, Theodor Bilharz Research Institute, Giza, Egypt.Department of Anesthesiology, Theodor Bilharz Research Institute, Giza, Egypt.Department of Anesthesiology, Theodor Bilharz Research Institute, Giza, Egypt.Department of Clinical and Chemical Pathology, Theodor Bilharz Research Institute, Giza, Egypt.Department of Clinical and Chemical Pathology, Theodor Bilharz Research Institute, Giza, Egypt.Department of Anesthesiology, Faculty of Medicine, Cairo University, Ministry of High Education and Scientific Research, Giza, Egypt.Department of Anesthesiology, Theodor Bilharz Research Institute, Giza, Egypt.BackgroundThis study was designed to measure in vivo effects of propofol, isoflurane and sevoflurane on apoptosis by measuring caspase-3 and tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL) blood level as apoptotic markers.MethodsAfter obtaining ethical committee approval and informed written consents, sixty adult patients ASA I scheduled for open cholecystectomy participated in this study. They were randomally allocated into one of three equal groups to receive propofol infusion, low-flow isoflurane or sevoflurane for maintenance of anesthesia. Venous blood samples were collected preoperatively, immediately postoperative and after 24 hours to measure hemoglobin, hematocrit, creatinine, liver enzymes, serum TRAIL and caspase-3 levels.ResultsThere was no significant difference in hematological markers and serum creatinine. Liver enzymes showed significant postoperative rise (P < 0.05). In Propofol group, TRAIL and caspase-3 levels were significantly elevated immediately postoperative then decreased significantly after 24-hours (P < 0.05). In Isoflurane group, immediate postoperative level of TRAIL was significantly higher than 24 hours reading and significantly lower than its level in Propofol group at the same timing meanwhile caspase-3 levels were comparable at different timings. In Sevoflurane group, TRAIL and caspase-3 levels increased significantly in both postoperative samples than preoperative level and than those of Isoflurane and Propofol groups after 24 hours concerning TRAIL (P & 0.05).ConclusionsThis study concluded that isoflurane is superior and sevoflurane is the least effective among the three anesthetics in protection against apoptosis. This study neither proved nor excluded propofol-induced apoptosis. Further studies are required during lengthy procedure and in compromised patients.http://ekja.org/upload/pdf/kjae-63-18.pdfapoptosisin vivoisofluranepropofolsevoflurane |
| spellingShingle | Emad S. Osman Hanan F. Khafagy Yasser M. Samhan Mona M. Hassan Faten M. El-Shanawany Abdel Rahman M. Fathallah Gehan G. El-fandy In vivo effects of different anesthetic agents on apoptosis Korean Journal of Anesthesiology apoptosis in vivo isoflurane propofol sevoflurane |
| title | In vivo effects of different anesthetic agents on apoptosis |
| title_full | In vivo effects of different anesthetic agents on apoptosis |
| title_fullStr | In vivo effects of different anesthetic agents on apoptosis |
| title_full_unstemmed | In vivo effects of different anesthetic agents on apoptosis |
| title_short | In vivo effects of different anesthetic agents on apoptosis |
| title_sort | in vivo effects of different anesthetic agents on apoptosis |
| topic | apoptosis in vivo isoflurane propofol sevoflurane |
| url | http://ekja.org/upload/pdf/kjae-63-18.pdf |
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