Genetic variability of beech populations in Iran assessed by PCR-RFLP analysis of cp-DNA
Fagus is one of the most abundant and economically important forest tree genera in north of Iran. 14 populations of F. orientalis Lipsky were selected from its wide distribution through the Caspian Forests, in order to study the species genetic variation by three methods: enzyme, cpDNA and morpholog...
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| Format: | Article |
| Language: | fas |
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Research Institute of Forests and Rangelands of Iran
2003-09-01
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| Series: | تحقیقات جنگل و صنوبر ایران |
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| Online Access: | http://ijfpr.areeo.ac.ir/article_109360_972898cfc22e63a4353654f9e259d996.pdf |
| _version_ | 1819104866236104704 |
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| author | Parvin Salehi Shanjani |
| author_facet | Parvin Salehi Shanjani |
| author_sort | Parvin Salehi Shanjani |
| collection | DOAJ |
| description | Fagus is one of the most abundant and economically important forest tree genera in north of Iran. 14 populations of F. orientalis Lipsky were selected from its wide distribution through the Caspian Forests, in order to study the species genetic variation by three methods: enzyme, cpDNA and morphology. Chloroplast (cp) DNA polymorphysm was analyzed in the same populations of the Iranian beech by PCR-RFLP test. Two cpDNA gene regions, including DT and OA were generated and digested by bounder of HaeIII and HinfI. endonucleases The generated pieces fragments were separated by gel electrophoresis. The digestied profiles of the two regions CDT and OA were compared simultaneously with the digestied fragments of the control DNA of the known haplotype (kindly provided by Raffaello Giannini, IMGPF-CNR, Italy) to identify the Iranian haplotypes. The restricted fragments of the region DT did not show polymorphism among the individuals within any analysed population. However, among individuals within the analysed populations of Asalem and Neka-1400 areas, polymorphism in the bounder fragments of the OA region was found. Overall, three different chloroplast (cp) haplotypes were scored. Distribution of the cpDNA haplotypes revealed a geographical structure of the genetic differentiation with Gst=%68.7 and Nst=%70.3. |
| first_indexed | 2024-12-22T02:13:09Z |
| format | Article |
| id | doaj.art-1cf849896fc84a88a637c0549bbd6fef |
| institution | Directory Open Access Journal |
| issn | 1735-0883 2383-1146 |
| language | fas |
| last_indexed | 2024-12-22T02:13:09Z |
| publishDate | 2003-09-01 |
| publisher | Research Institute of Forests and Rangelands of Iran |
| record_format | Article |
| series | تحقیقات جنگل و صنوبر ایران |
| spelling | doaj.art-1cf849896fc84a88a637c0549bbd6fef2022-12-21T18:42:20ZfasResearch Institute of Forests and Rangelands of Iranتحقیقات جنگل و صنوبر ایران1735-08832383-11462003-09-0111344747010.22092/ijfpr.2003.109360109360Genetic variability of beech populations in Iran assessed by PCR-RFLP analysis of cp-DNAParvin Salehi Shanjani0Forest Division, Research Institute of Forests and Rangelands, Tehran, IranFagus is one of the most abundant and economically important forest tree genera in north of Iran. 14 populations of F. orientalis Lipsky were selected from its wide distribution through the Caspian Forests, in order to study the species genetic variation by three methods: enzyme, cpDNA and morphology. Chloroplast (cp) DNA polymorphysm was analyzed in the same populations of the Iranian beech by PCR-RFLP test. Two cpDNA gene regions, including DT and OA were generated and digested by bounder of HaeIII and HinfI. endonucleases The generated pieces fragments were separated by gel electrophoresis. The digestied profiles of the two regions CDT and OA were compared simultaneously with the digestied fragments of the control DNA of the known haplotype (kindly provided by Raffaello Giannini, IMGPF-CNR, Italy) to identify the Iranian haplotypes. The restricted fragments of the region DT did not show polymorphism among the individuals within any analysed population. However, among individuals within the analysed populations of Asalem and Neka-1400 areas, polymorphism in the bounder fragments of the OA region was found. Overall, three different chloroplast (cp) haplotypes were scored. Distribution of the cpDNA haplotypes revealed a geographical structure of the genetic differentiation with Gst=%68.7 and Nst=%70.3.http://ijfpr.areeo.ac.ir/article_109360_972898cfc22e63a4353654f9e259d996.pdfFagus orientalisHyrcanian zonegenetic variabilitycpDNA characteristics |
| spellingShingle | Parvin Salehi Shanjani Genetic variability of beech populations in Iran assessed by PCR-RFLP analysis of cp-DNA تحقیقات جنگل و صنوبر ایران Fagus orientalis Hyrcanian zone genetic variability cpDNA characteristics |
| title | Genetic variability of beech populations in Iran assessed by PCR-RFLP analysis of cp-DNA |
| title_full | Genetic variability of beech populations in Iran assessed by PCR-RFLP analysis of cp-DNA |
| title_fullStr | Genetic variability of beech populations in Iran assessed by PCR-RFLP analysis of cp-DNA |
| title_full_unstemmed | Genetic variability of beech populations in Iran assessed by PCR-RFLP analysis of cp-DNA |
| title_short | Genetic variability of beech populations in Iran assessed by PCR-RFLP analysis of cp-DNA |
| title_sort | genetic variability of beech populations in iran assessed by pcr rflp analysis of cp dna |
| topic | Fagus orientalis Hyrcanian zone genetic variability cpDNA characteristics |
| url | http://ijfpr.areeo.ac.ir/article_109360_972898cfc22e63a4353654f9e259d996.pdf |
| work_keys_str_mv | AT parvinsalehishanjani geneticvariabilityofbeechpopulationsiniranassessedbypcrrflpanalysisofcpdna |