The mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer
Background and objective It has been confirmed that nm23-H1 gene is one of the tumor metastasis suppressor genes. Up to now, the exact mechanism of nm23-H1 gebe is uncertain. The aim of this study the mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer. Meth...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2008-10-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2008.05.007&path%5B%5D=160 |
| _version_ | 1828238399959465984 |
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| author | Xueqin YANG Yibing YAO Wen ZHU Li MA Daxing ZHU Wen XIAO Changlin LI Jiabao MA Zhixuan ZHANG Liwei GAO Yanping WANG Qinghua ZHOU |
| author_facet | Xueqin YANG Yibing YAO Wen ZHU Li MA Daxing ZHU Wen XIAO Changlin LI Jiabao MA Zhixuan ZHANG Liwei GAO Yanping WANG Qinghua ZHOU |
| author_sort | Xueqin YANG |
| collection | DOAJ |
| description | Background and objective It has been confirmed that nm23-H1 gene is one of the tumor metastasis suppressor genes. Up to now, the exact mechanism of nm23-H1 gebe is uncertain. The aim of this study the mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer. Methods The wild and mutant typeof pEGFP-nm23-H1 plasmids [WT (wild type), H118F, S120G, P96S, S44A] were transfected into the L9981 lung cancer cell lines through liposome method, and the complex of KSR and nm23-H1 was detected through co-immunoprecipitation and Western blot assay. Results The human KSR could be detected in the nm23-H1 immunoprecipitations in all the trasfected L9981 lung cancer cell lines. But no significant difference of KSR expression was found in the wild and mutantnm23-H1 trasfected cell lines (F =0.190, P =0.938). Conclusion There was a close interaction between nm23-H1 and KSR, which was independent of the nm23-H1 mutation. Nm23-H1 involving in the Ras signaling of lung cancer may be through the KSR gene. |
| first_indexed | 2024-04-12T21:04:28Z |
| format | Article |
| id | doaj.art-1cfba8abd0ac43a7aeae7547b76be95f |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-04-12T21:04:28Z |
| publishDate | 2008-10-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-1cfba8abd0ac43a7aeae7547b76be95f2022-12-22T03:16:45ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872008-10-01115700703The mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancerXueqin YANGYibing YAOWen ZHULi MADaxing ZHUWen XIAOChanglin LIJiabao MAZhixuan ZHANGLiwei GAOYanping WANGQinghua ZHOUBackground and objective It has been confirmed that nm23-H1 gene is one of the tumor metastasis suppressor genes. Up to now, the exact mechanism of nm23-H1 gebe is uncertain. The aim of this study the mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer. Methods The wild and mutant typeof pEGFP-nm23-H1 plasmids [WT (wild type), H118F, S120G, P96S, S44A] were transfected into the L9981 lung cancer cell lines through liposome method, and the complex of KSR and nm23-H1 was detected through co-immunoprecipitation and Western blot assay. Results The human KSR could be detected in the nm23-H1 immunoprecipitations in all the trasfected L9981 lung cancer cell lines. But no significant difference of KSR expression was found in the wild and mutantnm23-H1 trasfected cell lines (F =0.190, P =0.938). Conclusion There was a close interaction between nm23-H1 and KSR, which was independent of the nm23-H1 mutation. Nm23-H1 involving in the Ras signaling of lung cancer may be through the KSR gene.http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2008.05.007&path%5B%5D=160nm23-H1KSRLung neoplasmsRas signaling |
| spellingShingle | Xueqin YANG Yibing YAO Wen ZHU Li MA Daxing ZHU Wen XIAO Changlin LI Jiabao MA Zhixuan ZHANG Liwei GAO Yanping WANG Qinghua ZHOU The mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer Chinese Journal of Lung Cancer nm23-H1 KSR Lung neoplasms Ras signaling |
| title | The mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer |
| title_full | The mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer |
| title_fullStr | The mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer |
| title_full_unstemmed | The mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer |
| title_short | The mechanism of metastasis suppressor gene nm23-H1 involving in the Ras signaling of lung cancer |
| title_sort | mechanism of metastasis suppressor gene nm23 h1 involving in the ras signaling of lung cancer |
| topic | nm23-H1 KSR Lung neoplasms Ras signaling |
| url | http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2008.05.007&path%5B%5D=160 |
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