Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorpha
Eukaryotic life cycles alternate between haploid and diploid phases and in phylogenetically diverse unicellular eukaryotes, expression of paralogous homeodomain genes in gametes primes the haploid-to-diploid transition. In the unicellular chlorophyte alga Chlamydomonas, KNOX and BELL TALE-homeodomai...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-09-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/57088 |
| _version_ | 1797991943904428032 |
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| author | Tom Dierschke Eduardo Flores-Sandoval Madlen I Rast-Somssich Felix Althoff Sabine Zachgo John L Bowman |
| author_facet | Tom Dierschke Eduardo Flores-Sandoval Madlen I Rast-Somssich Felix Althoff Sabine Zachgo John L Bowman |
| author_sort | Tom Dierschke |
| collection | DOAJ |
| description | Eukaryotic life cycles alternate between haploid and diploid phases and in phylogenetically diverse unicellular eukaryotes, expression of paralogous homeodomain genes in gametes primes the haploid-to-diploid transition. In the unicellular chlorophyte alga Chlamydomonas, KNOX and BELL TALE-homeodomain genes mediate this transition. We demonstrate that in the liverwort Marchantia polymorpha, paternal (sperm) expression of three of five phylogenetically diverse BELL genes, MpBELL234, and maternal (egg) expression of both MpKNOX1 and MpBELL34 mediate the haploid-to-diploid transition. Loss-of-function alleles of MpKNOX1 result in zygotic arrest, whereas a loss of either maternal or paternal MpBELL234 results in variable zygotic and early embryonic arrest. Expression of MpKNOX1 and MpBELL34 during diploid sporophyte development is consistent with a later role for these genes in patterning the sporophyte. These results indicate that the ancestral mechanism to activate diploid gene expression was retained in early diverging land plants and subsequently co-opted during evolution of the diploid sporophyte body. |
| first_indexed | 2024-04-11T08:59:08Z |
| format | Article |
| id | doaj.art-1cfcfb3d116a435489e1e6c217426001 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T08:59:08Z |
| publishDate | 2021-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-1cfcfb3d116a435489e1e6c2174260012022-12-22T04:32:49ZengeLife Sciences Publications LtdeLife2050-084X2021-09-011010.7554/eLife.57088Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorphaTom Dierschke0https://orcid.org/0000-0002-3547-5765Eduardo Flores-Sandoval1Madlen I Rast-Somssich2Felix Althoff3Sabine Zachgo4John L Bowman5https://orcid.org/0000-0001-7347-3691School of Biological Sciences, Monash University, Melbourne, Australia; Botany Department, University of Osnabrück, Osnabrück, GermanySchool of Biological Sciences, Monash University, Melbourne, AustraliaSchool of Biological Sciences, Monash University, Melbourne, AustraliaBotany Department, University of Osnabrück, Osnabrück, GermanyBotany Department, University of Osnabrück, Osnabrück, GermanySchool of Biological Sciences, Monash University, Melbourne, AustraliaEukaryotic life cycles alternate between haploid and diploid phases and in phylogenetically diverse unicellular eukaryotes, expression of paralogous homeodomain genes in gametes primes the haploid-to-diploid transition. In the unicellular chlorophyte alga Chlamydomonas, KNOX and BELL TALE-homeodomain genes mediate this transition. We demonstrate that in the liverwort Marchantia polymorpha, paternal (sperm) expression of three of five phylogenetically diverse BELL genes, MpBELL234, and maternal (egg) expression of both MpKNOX1 and MpBELL34 mediate the haploid-to-diploid transition. Loss-of-function alleles of MpKNOX1 result in zygotic arrest, whereas a loss of either maternal or paternal MpBELL234 results in variable zygotic and early embryonic arrest. Expression of MpKNOX1 and MpBELL34 during diploid sporophyte development is consistent with a later role for these genes in patterning the sporophyte. These results indicate that the ancestral mechanism to activate diploid gene expression was retained in early diverging land plants and subsequently co-opted during evolution of the diploid sporophyte body.https://elifesciences.org/articles/57088TALE-homeodomainplant evolutionMarchantiaalternation of generations |
| spellingShingle | Tom Dierschke Eduardo Flores-Sandoval Madlen I Rast-Somssich Felix Althoff Sabine Zachgo John L Bowman Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorpha eLife TALE-homeodomain plant evolution Marchantia alternation of generations |
| title | Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorpha |
| title_full | Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorpha |
| title_fullStr | Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorpha |
| title_full_unstemmed | Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorpha |
| title_short | Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorpha |
| title_sort | gamete expression of tale class hd genes activates the diploid sporophyte program in marchantia polymorpha |
| topic | TALE-homeodomain plant evolution Marchantia alternation of generations |
| url | https://elifesciences.org/articles/57088 |
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