HGF and c-Met interaction promotes migration in human chondrosarcoma cells.
Chondrosarcoma is a type of highly malignant tumor with a potent capacity for local invasion and causing distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. Hepatocyte growth factor (HGF) has been demonstrated to stimulate cancer proliferation, migration, and metasta...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3540013?pdf=render |
| _version_ | 1811236530104041472 |
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| author | Hsi-Kai Tsou Hsien-Te Chen Ya-Huey Hung Chia-Hao Chang Te-Mao Li Yi-Chin Fong Chih-Hsin Tang |
| author_facet | Hsi-Kai Tsou Hsien-Te Chen Ya-Huey Hung Chia-Hao Chang Te-Mao Li Yi-Chin Fong Chih-Hsin Tang |
| author_sort | Hsi-Kai Tsou |
| collection | DOAJ |
| description | Chondrosarcoma is a type of highly malignant tumor with a potent capacity for local invasion and causing distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. Hepatocyte growth factor (HGF) has been demonstrated to stimulate cancer proliferation, migration, and metastasis. However, the effect of HGF on migration activity of human chondrosarcoma cells is not well known. Here, we found that human chondrosarcoma tissues demonstrated significant expression of HGF, which was higher than that in normal cartilage. We also found that HGF increased the migration and expression of matrix metalloproteinase (MMP)-2 in human chondrosarcoma cells. c-Met inhibitor and siRNA reduced HGF-increased cell migration and MMP-2 expression. HGF treatment resulted in activation of the phosphatidylinositol 3'-kinase (PI3K)/Akt/PKCδ/NF-κB pathway, and HGF-induced expression of MMP-2 and cell migration was inhibited by specific inhibitors or siRNA-knockdown of PI3K, Akt, PKCδ, and NF-κB cascades. Taken together, our results indicated that HGF enhances migration of chondrosarcoma cells by increasing MMP-2 expression through the c-Met receptor/PI3K/Akt/PKCδ/NF-κB signal transduction pathway. |
| first_indexed | 2024-04-12T12:09:53Z |
| format | Article |
| id | doaj.art-1cfeac8c714f49b1847e841c6c029b76 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T12:09:53Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1cfeac8c714f49b1847e841c6c029b762022-12-22T03:33:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5397410.1371/journal.pone.0053974HGF and c-Met interaction promotes migration in human chondrosarcoma cells.Hsi-Kai TsouHsien-Te ChenYa-Huey HungChia-Hao ChangTe-Mao LiYi-Chin FongChih-Hsin TangChondrosarcoma is a type of highly malignant tumor with a potent capacity for local invasion and causing distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. Hepatocyte growth factor (HGF) has been demonstrated to stimulate cancer proliferation, migration, and metastasis. However, the effect of HGF on migration activity of human chondrosarcoma cells is not well known. Here, we found that human chondrosarcoma tissues demonstrated significant expression of HGF, which was higher than that in normal cartilage. We also found that HGF increased the migration and expression of matrix metalloproteinase (MMP)-2 in human chondrosarcoma cells. c-Met inhibitor and siRNA reduced HGF-increased cell migration and MMP-2 expression. HGF treatment resulted in activation of the phosphatidylinositol 3'-kinase (PI3K)/Akt/PKCδ/NF-κB pathway, and HGF-induced expression of MMP-2 and cell migration was inhibited by specific inhibitors or siRNA-knockdown of PI3K, Akt, PKCδ, and NF-κB cascades. Taken together, our results indicated that HGF enhances migration of chondrosarcoma cells by increasing MMP-2 expression through the c-Met receptor/PI3K/Akt/PKCδ/NF-κB signal transduction pathway.http://europepmc.org/articles/PMC3540013?pdf=render |
| spellingShingle | Hsi-Kai Tsou Hsien-Te Chen Ya-Huey Hung Chia-Hao Chang Te-Mao Li Yi-Chin Fong Chih-Hsin Tang HGF and c-Met interaction promotes migration in human chondrosarcoma cells. PLoS ONE |
| title | HGF and c-Met interaction promotes migration in human chondrosarcoma cells. |
| title_full | HGF and c-Met interaction promotes migration in human chondrosarcoma cells. |
| title_fullStr | HGF and c-Met interaction promotes migration in human chondrosarcoma cells. |
| title_full_unstemmed | HGF and c-Met interaction promotes migration in human chondrosarcoma cells. |
| title_short | HGF and c-Met interaction promotes migration in human chondrosarcoma cells. |
| title_sort | hgf and c met interaction promotes migration in human chondrosarcoma cells |
| url | http://europepmc.org/articles/PMC3540013?pdf=render |
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