Trichinella T9 in wild bears in Japan: Prevalence, species/genotype identification, and public health implications
In Japan, the recent series of sporadic outbreaks of human trichinellosis caused by Trichinella (Nematoda: Trichocephalida) has occurred owing to the consumption of raw or insufficiently cooked meat from wild bears. However, the infection status and molecular characteristics of Trichinella larvae in...
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| Format: | Article |
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Elsevier
2023-08-01
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| Series: | International Journal for Parasitology: Parasites and Wildlife |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213224423000548 |
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| author | Masaki Murakami Toshihiro Tokiwa Hiromu Sugiyama Mitsuko Shiroyama Yasuyuki Morishima Sota Watanabe Takato Sasamori Mami Kondo Tsutomu Mano Hifumi Tsuruga |
| author_facet | Masaki Murakami Toshihiro Tokiwa Hiromu Sugiyama Mitsuko Shiroyama Yasuyuki Morishima Sota Watanabe Takato Sasamori Mami Kondo Tsutomu Mano Hifumi Tsuruga |
| author_sort | Masaki Murakami |
| collection | DOAJ |
| description | In Japan, the recent series of sporadic outbreaks of human trichinellosis caused by Trichinella (Nematoda: Trichocephalida) has occurred owing to the consumption of raw or insufficiently cooked meat from wild bears. However, the infection status and molecular characteristics of Trichinella larvae in Japanese wild bears remain poorly understood. This study investigated the prevalence of Trichinella spp. in brown bears (Ursus arctos) from Hokkaido, and Japanese black bears (Ursus thibetanus japonicus) from three prefectures (Aomori, Akita, and Iwate) in northern Japan, between April 2019 and August 2022. Trichinella larvae were detected in 2.5% (6/236) of the brown bears and 0.9% (1/117) of the Japanese black bears. Sequence analysis using two genetic loci, the internal transcribed spacer region of nuclear ribosomal DNA and the mitochondrial cytochrome c oxidase subunit I gene, revealed that the larvae collected from the seven infected bears were identical to one of the two haplotypes of Trichinella T9. The prevalence of Trichinella T9 is low but is maintained in bears in the Hokkaido and Iwate prefectures suggesting that undercooked meat from these animals could cause human infection. Thus, continued health education campaigns are needed to raise awareness of the potential risk of trichinellosis among hunters, meat suppliers, consumers, and local governmental health agencies. |
| first_indexed | 2024-03-12T17:10:59Z |
| format | Article |
| id | doaj.art-1cff0f7cffad468f91bd5166af93f089 |
| institution | Directory Open Access Journal |
| issn | 2213-2244 |
| language | English |
| last_indexed | 2024-03-12T17:10:59Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Parasites and Wildlife |
| spelling | doaj.art-1cff0f7cffad468f91bd5166af93f0892023-08-06T04:37:21ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442023-08-0121264268Trichinella T9 in wild bears in Japan: Prevalence, species/genotype identification, and public health implicationsMasaki Murakami0Toshihiro Tokiwa1Hiromu Sugiyama2Mitsuko Shiroyama3Yasuyuki Morishima4Sota Watanabe5Takato Sasamori6Mami Kondo7Tsutomu Mano8Hifumi Tsuruga9Laboratory of Veterinary Parasitology, Nippon Veterinary and Life Science University, Musashino, Tokyo, 180-8602, Japan; Laboratory of Helminthology, Department of Parasitology, National Institute of Infectious Diseases, Shinjuku, Tokyo, 162-8640, JapanLaboratory of Veterinary Parasitology, Nippon Veterinary and Life Science University, Musashino, Tokyo, 180-8602, Japan; Corresponding author.Laboratory of Helminthology, Department of Parasitology, National Institute of Infectious Diseases, Shinjuku, Tokyo, 162-8640, Japan; School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa, 252-5201, Japan; Corresponding author. Laboratory of Helminthology, Department of Parasitology, National Institute of Infectious Diseases, Shinjuku, Tokyo, 162-8640, Japan.School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa, 252-5201, JapanLaboratory of Helminthology, Department of Parasitology, National Institute of Infectious Diseases, Shinjuku, Tokyo, 162-8640, JapanGraduate School of Arts and Science, Iwate University, Morioka, Iwate, 020-8550, JapanAgriculture, Forestry and Fisheries Division, Fukaura Town Office, Fukaura, Aomori, 038-2324, JapanNature Conservation Division, Department of Living and Environment, Akita Prefectural Office, Akita, Akita, 010-8570, JapanResearch Institute of Energy, Environment and Geology, Industrial Technology and Environment Research Department, Hokkaido Research Organization, Sapporo, Hokkaido, 060-0819, JapanResearch Institute of Energy, Environment and Geology, Industrial Technology and Environment Research Department, Hokkaido Research Organization, Sapporo, Hokkaido, 060-0819, JapanIn Japan, the recent series of sporadic outbreaks of human trichinellosis caused by Trichinella (Nematoda: Trichocephalida) has occurred owing to the consumption of raw or insufficiently cooked meat from wild bears. However, the infection status and molecular characteristics of Trichinella larvae in Japanese wild bears remain poorly understood. This study investigated the prevalence of Trichinella spp. in brown bears (Ursus arctos) from Hokkaido, and Japanese black bears (Ursus thibetanus japonicus) from three prefectures (Aomori, Akita, and Iwate) in northern Japan, between April 2019 and August 2022. Trichinella larvae were detected in 2.5% (6/236) of the brown bears and 0.9% (1/117) of the Japanese black bears. Sequence analysis using two genetic loci, the internal transcribed spacer region of nuclear ribosomal DNA and the mitochondrial cytochrome c oxidase subunit I gene, revealed that the larvae collected from the seven infected bears were identical to one of the two haplotypes of Trichinella T9. The prevalence of Trichinella T9 is low but is maintained in bears in the Hokkaido and Iwate prefectures suggesting that undercooked meat from these animals could cause human infection. Thus, continued health education campaigns are needed to raise awareness of the potential risk of trichinellosis among hunters, meat suppliers, consumers, and local governmental health agencies.http://www.sciencedirect.com/science/article/pii/S2213224423000548BearGame meatPublic healthTrichinellaUrsus thibetanus japonicusUrsus arctos |
| spellingShingle | Masaki Murakami Toshihiro Tokiwa Hiromu Sugiyama Mitsuko Shiroyama Yasuyuki Morishima Sota Watanabe Takato Sasamori Mami Kondo Tsutomu Mano Hifumi Tsuruga Trichinella T9 in wild bears in Japan: Prevalence, species/genotype identification, and public health implications International Journal for Parasitology: Parasites and Wildlife Bear Game meat Public health Trichinella Ursus thibetanus japonicus Ursus arctos |
| title | Trichinella T9 in wild bears in Japan: Prevalence, species/genotype identification, and public health implications |
| title_full | Trichinella T9 in wild bears in Japan: Prevalence, species/genotype identification, and public health implications |
| title_fullStr | Trichinella T9 in wild bears in Japan: Prevalence, species/genotype identification, and public health implications |
| title_full_unstemmed | Trichinella T9 in wild bears in Japan: Prevalence, species/genotype identification, and public health implications |
| title_short | Trichinella T9 in wild bears in Japan: Prevalence, species/genotype identification, and public health implications |
| title_sort | trichinella t9 in wild bears in japan prevalence species genotype identification and public health implications |
| topic | Bear Game meat Public health Trichinella Ursus thibetanus japonicus Ursus arctos |
| url | http://www.sciencedirect.com/science/article/pii/S2213224423000548 |
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