Immunogenicity and Blocking Efficacy of Norovirus GII.4 Recombinant P Protein Vaccine
Noroviruses (NoVs) are the main cause of acute gastroenteritis in all ages worldwide. The aim of this study was to produce the recombinant P protein of norovirus and to demonstrate its blocking effect. In this study, the engineered strains were induced to express the P protein of NoVs GII.4, which w...
| Κύριοι συγγραφείς: | , , , , , , |
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| Μορφή: | Άρθρο |
| Γλώσσα: | English |
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MDPI AG
2023-06-01
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| Σειρά: | Vaccines |
| Θέματα: | |
| Διαθέσιμο Online: | https://www.mdpi.com/2076-393X/11/6/1053 |
| _version_ | 1827735423846187008 |
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| author | Zhendi Yu Qingyi Shao Zhangkai Xu Chenghao Chen Mingfan Li Yi Jiang Dongqing Cheng |
| author_facet | Zhendi Yu Qingyi Shao Zhangkai Xu Chenghao Chen Mingfan Li Yi Jiang Dongqing Cheng |
| author_sort | Zhendi Yu |
| collection | DOAJ |
| description | Noroviruses (NoVs) are the main cause of acute gastroenteritis in all ages worldwide. The aim of this study was to produce the recombinant P protein of norovirus and to demonstrate its blocking effect. In this study, the engineered strains were induced to express the P protein of NoVs GII.4, which was identified using SDS-PAGE and ELISA as having the capacity to bind to histo-blood group antigens (HBGAs). Rabbits were immunized to obtain neutralizing antibodies. ELISA and ISC-RT-qPCR were used to determine the blocking efficacy of the neutralizing antibody to human norovirus (HuNoV) and murine norovirus (MNV). The recombinant P protein (35 KD) was obtained, and the neutralizing antibody was successfully prepared. The neutralizing antibody could block the binding of the P protein and HuNoV to HBGAs. Neutralizing antibodies can also block MNV invasion into host cells RAW264.7. The recombinant P protein expressed in <i>E. coli</i> can induce antibodies to block HuNoV and MNV. The recombinant P protein of NoVs GII.4 has the value of vaccine development. |
| first_indexed | 2024-03-11T01:50:43Z |
| format | Article |
| id | doaj.art-1cff6eb73aa842de9608b0ca47f853be |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T01:50:43Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-1cff6eb73aa842de9608b0ca47f853be2023-11-18T12:58:33ZengMDPI AGVaccines2076-393X2023-06-01116105310.3390/vaccines11061053Immunogenicity and Blocking Efficacy of Norovirus GII.4 Recombinant P Protein VaccineZhendi Yu0Qingyi Shao1Zhangkai Xu2Chenghao Chen3Mingfan Li4Yi Jiang5Dongqing Cheng6School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaSchool of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaNoroviruses (NoVs) are the main cause of acute gastroenteritis in all ages worldwide. The aim of this study was to produce the recombinant P protein of norovirus and to demonstrate its blocking effect. In this study, the engineered strains were induced to express the P protein of NoVs GII.4, which was identified using SDS-PAGE and ELISA as having the capacity to bind to histo-blood group antigens (HBGAs). Rabbits were immunized to obtain neutralizing antibodies. ELISA and ISC-RT-qPCR were used to determine the blocking efficacy of the neutralizing antibody to human norovirus (HuNoV) and murine norovirus (MNV). The recombinant P protein (35 KD) was obtained, and the neutralizing antibody was successfully prepared. The neutralizing antibody could block the binding of the P protein and HuNoV to HBGAs. Neutralizing antibodies can also block MNV invasion into host cells RAW264.7. The recombinant P protein expressed in <i>E. coli</i> can induce antibodies to block HuNoV and MNV. The recombinant P protein of NoVs GII.4 has the value of vaccine development.https://www.mdpi.com/2076-393X/11/6/1053norovirusrecombinant P proteinneutralizing antibodyimmunogenicityblocking efficacy |
| spellingShingle | Zhendi Yu Qingyi Shao Zhangkai Xu Chenghao Chen Mingfan Li Yi Jiang Dongqing Cheng Immunogenicity and Blocking Efficacy of Norovirus GII.4 Recombinant P Protein Vaccine Vaccines norovirus recombinant P protein neutralizing antibody immunogenicity blocking efficacy |
| title | Immunogenicity and Blocking Efficacy of Norovirus GII.4 Recombinant P Protein Vaccine |
| title_full | Immunogenicity and Blocking Efficacy of Norovirus GII.4 Recombinant P Protein Vaccine |
| title_fullStr | Immunogenicity and Blocking Efficacy of Norovirus GII.4 Recombinant P Protein Vaccine |
| title_full_unstemmed | Immunogenicity and Blocking Efficacy of Norovirus GII.4 Recombinant P Protein Vaccine |
| title_short | Immunogenicity and Blocking Efficacy of Norovirus GII.4 Recombinant P Protein Vaccine |
| title_sort | immunogenicity and blocking efficacy of norovirus gii 4 recombinant p protein vaccine |
| topic | norovirus recombinant P protein neutralizing antibody immunogenicity blocking efficacy |
| url | https://www.mdpi.com/2076-393X/11/6/1053 |
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