Comparison of ISSR and AFLP markers in assessing genetic diversity among Nettle (Urtica dioica L.) populations.
Urtica dioica is an important medicinal plant which is widely distributed in Mazandaran province (North of Iran). In this study for the first time Amplified Fragment Length Polymorphism (AFLP) and Inter-simple Sequence Repeat (ISSR) markers were used for detection of genetic polymorphism in Mazandaran...
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| Format: | Article |
| Language: | English |
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Genetics and Agricultural Biotechnology Institute of Tabarestan
2016-06-01
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| Series: | Journal of Plant Molecular Breeding |
| Subjects: | |
| Online Access: | http://www.jpmb-gabit.ir/article_19659_7e84fe0f1d821807efa0e37801b69eef.pdf |
| _version_ | 1830479008579452928 |
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| author | Mostafa Haghpanah Seyed Kamal Kazemitabar Seyed Hamidreza Hashemi Seyed Mohammad Alavi |
| author_facet | Mostafa Haghpanah Seyed Kamal Kazemitabar Seyed Hamidreza Hashemi Seyed Mohammad Alavi |
| author_sort | Mostafa Haghpanah |
| collection | DOAJ |
| description | Urtica dioica is an important medicinal plant which is widely distributed in Mazandaran province (North of Iran). In this study for the first time Amplified Fragment Length Polymorphism (AFLP) and Inter-simple Sequence Repeat (ISSR) markers were used for detection of genetic polymorphism in Mazandaran nettle. Ten AFLP primer combinations and seventeen ISSR markers were utilized. AFLP produced 830 scorable bands out of which 90.21% were polymorphic. ISSR primers amplified 234 bands, 181 being polymorphic (77.3%). Average heterozygosity for AFLP and ISSR markers were 0.25, 0.23 respectively. Marker Index obtains 22.25 for AFLP and 15.57 for ISSR. The number of cluster computed was same for both molecular makers but location of samples in branch were different. The total compare of these two marker systems shown AFLP marker was a useful tool for detection of U. dioica’sgenetic diversity. This plant is very variable and is genetically distinct in east, west and north of Mazandaran. |
| first_indexed | 2024-12-21T16:38:39Z |
| format | Article |
| id | doaj.art-1d024879bd614f52a32cde4a3a4bcc09 |
| institution | Directory Open Access Journal |
| issn | 2322-3332 2322-5092 |
| language | English |
| last_indexed | 2024-12-21T16:38:39Z |
| publishDate | 2016-06-01 |
| publisher | Genetics and Agricultural Biotechnology Institute of Tabarestan |
| record_format | Article |
| series | Journal of Plant Molecular Breeding |
| spelling | doaj.art-1d024879bd614f52a32cde4a3a4bcc092022-12-21T18:57:09ZengGenetics and Agricultural Biotechnology Institute of TabarestanJournal of Plant Molecular Breeding2322-33322322-50922016-06-0141101610.22058/jpmb.2016.1965919659Comparison of ISSR and AFLP markers in assessing genetic diversity among Nettle (Urtica dioica L.) populations.Mostafa Haghpanah0Seyed Kamal Kazemitabar1Seyed Hamidreza Hashemi2Seyed Mohammad Alavi3Department of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, IranDepartment of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, IranGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari, IranGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari, IranUrtica dioica is an important medicinal plant which is widely distributed in Mazandaran province (North of Iran). In this study for the first time Amplified Fragment Length Polymorphism (AFLP) and Inter-simple Sequence Repeat (ISSR) markers were used for detection of genetic polymorphism in Mazandaran nettle. Ten AFLP primer combinations and seventeen ISSR markers were utilized. AFLP produced 830 scorable bands out of which 90.21% were polymorphic. ISSR primers amplified 234 bands, 181 being polymorphic (77.3%). Average heterozygosity for AFLP and ISSR markers were 0.25, 0.23 respectively. Marker Index obtains 22.25 for AFLP and 15.57 for ISSR. The number of cluster computed was same for both molecular makers but location of samples in branch were different. The total compare of these two marker systems shown AFLP marker was a useful tool for detection of U. dioica’sgenetic diversity. This plant is very variable and is genetically distinct in east, west and north of Mazandaran.http://www.jpmb-gabit.ir/article_19659_7e84fe0f1d821807efa0e37801b69eef.pdfMedicinal plantMolecular markersgenetic variation |
| spellingShingle | Mostafa Haghpanah Seyed Kamal Kazemitabar Seyed Hamidreza Hashemi Seyed Mohammad Alavi Comparison of ISSR and AFLP markers in assessing genetic diversity among Nettle (Urtica dioica L.) populations. Journal of Plant Molecular Breeding Medicinal plant Molecular markers genetic variation |
| title | Comparison of ISSR and AFLP markers in assessing genetic diversity among Nettle (Urtica dioica L.) populations. |
| title_full | Comparison of ISSR and AFLP markers in assessing genetic diversity among Nettle (Urtica dioica L.) populations. |
| title_fullStr | Comparison of ISSR and AFLP markers in assessing genetic diversity among Nettle (Urtica dioica L.) populations. |
| title_full_unstemmed | Comparison of ISSR and AFLP markers in assessing genetic diversity among Nettle (Urtica dioica L.) populations. |
| title_short | Comparison of ISSR and AFLP markers in assessing genetic diversity among Nettle (Urtica dioica L.) populations. |
| title_sort | comparison of issr and aflp markers in assessing genetic diversity among nettle urtica dioica l populations |
| topic | Medicinal plant Molecular markers genetic variation |
| url | http://www.jpmb-gabit.ir/article_19659_7e84fe0f1d821807efa0e37801b69eef.pdf |
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