| Summary: | This study was aimed at extraction optimization of antibacterial agents from Carica papaya seed against S. enteritidis, B. cereus, V. vulnificus and P. mirabilis as affected by sonication-assisted extraction (SAE), contact time (CT) and solid-to-solvent ratio (SSR). The principal component analysis (PCA) and individual evaluation approaches identified that no SAE, 8 CT and 1:10 SSR were the best treatments with the highest antibacterial potency. The PCA identified no SAE, 8 CT, and 1:5 SSR as the second-beat treatment. The yield, total phenolic compound (TPC), C18:1n9t and C16:1 free fatty acids (FAs) in no SAE, 8 CT and 1:10 SSR treatment inhibited B. cereus, V. vulnificus and P. mirabilis growths while C21:0 and C15:0 in 30 min SAE, 8 CT and 1:2 SSR inhibited S. enteritidis growth. The yield, TPC, C18:1n9t and C16:1 FAs, and C6:0 and C24:1n9, C20:1, C4:0 and C20:0 FAs had antagonistic effects on B. cereus, V. vulnificus and P. mirabilis growths. The C21:0, C15:0, C6:0 and C13:0, and C23:0, C20:0 and C11:0 FAs had antagonistic effects on S. enteritidis growth. The PCA also denoted that the MIC50 and MIC0 had a higher variation than MIC; hence, the former variables were better to use in PCA.
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