Potential of metagenomic next-generation sequencing in detecting infections of ICU patients
Background: Due to the limitations of traditional microbiological detection techniques in evaluating complicated infections in ICU patients, it is necessary to explore novel and effective methods to improve the clinical detection of ICU patients’ infections. Objective: This study aimed to evaluate t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-04-01
|
| Series: | Molecular and Cellular Probes |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0890850823000075 |
| _version_ | 1797788440806293504 |
|---|---|
| author | Yanxu Liang Qingguo Feng Kai Wei Xiaoming Hou Xiaotao Song Yuantao Li |
| author_facet | Yanxu Liang Qingguo Feng Kai Wei Xiaoming Hou Xiaotao Song Yuantao Li |
| author_sort | Yanxu Liang |
| collection | DOAJ |
| description | Background: Due to the limitations of traditional microbiological detection techniques in evaluating complicated infections in ICU patients, it is necessary to explore novel and effective methods to improve the clinical detection of ICU patients’ infections. Objective: This study aimed to evaluate the efficiency and specificity of mNGS in screening pathogens in the blood, deep phlegm, urine, and other sample types of ICU patients exploring an effective method for infection detection. Methods: A total of 56 ICU patients with 131 samples were included in this study. The sample types included blood, deep phlegm, urine, drainage, anal swabs, and other types. Samples were analyzed by both conventional detection method and mNGS tests. The diagnosis efficiency and consistency of the two methods were compared. The distribution of the identified pathogens was analyzed. Moreover, the clinical features of patients with mNGS-positive or mNGS-negative results were compared. Results: The positive rate of mNGS was 81.7% (107/131) including 3.1% (4/131) weakly positive, while the positive rate of traditional detection was only 30.5%, including 29 strong positive results and 11 weak positive results. Additionally, there were 41 patients chose to adjust anti-infection strategies according to the results of mNGS, which significantly saved treatment costs. The mNGS-positive patients showed a shorter ICU hospitalization and higher intention to adjust anti-infection strategies than the mNGS-negative patients. Conclusion: mNGS is of great potential for the pathogen detection of ICU patients, and has a higher detection rate than traditional detection methods. Further clinical application investigations can be carried out to expand the application of mNGS. |
| first_indexed | 2024-03-13T01:36:32Z |
| format | Article |
| id | doaj.art-1d053fc2635046c6b839a5bb6539cf79 |
| institution | Directory Open Access Journal |
| issn | 0890-8508 |
| language | English |
| last_indexed | 2024-03-13T01:36:32Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular and Cellular Probes |
| spelling | doaj.art-1d053fc2635046c6b839a5bb6539cf792023-07-04T05:09:08ZengElsevierMolecular and Cellular Probes0890-85082023-04-0168101898Potential of metagenomic next-generation sequencing in detecting infections of ICU patientsYanxu Liang0Qingguo Feng1Kai Wei2Xiaoming Hou3Xiaotao Song4Yuantao Li5Department of Critical Care Medicine, The Fifth Central Hospital of Tianjin, Tianjin, 300450, ChinaCorresponding author. Department of Critical Care Medicine, The Fifth Central Hospital of Tianjin, No. 41 Zhejiang Road, Tanggu, Binhai New Area, Tianjin 300450, China.; Department of Critical Care Medicine, The Fifth Central Hospital of Tianjin, Tianjin, 300450, ChinaDepartment of Critical Care Medicine, The Fifth Central Hospital of Tianjin, Tianjin, 300450, ChinaDepartment of Critical Care Medicine, The Fifth Central Hospital of Tianjin, Tianjin, 300450, ChinaDepartment of Critical Care Medicine, The Fifth Central Hospital of Tianjin, Tianjin, 300450, ChinaDepartment of Critical Care Medicine, The Fifth Central Hospital of Tianjin, Tianjin, 300450, ChinaBackground: Due to the limitations of traditional microbiological detection techniques in evaluating complicated infections in ICU patients, it is necessary to explore novel and effective methods to improve the clinical detection of ICU patients’ infections. Objective: This study aimed to evaluate the efficiency and specificity of mNGS in screening pathogens in the blood, deep phlegm, urine, and other sample types of ICU patients exploring an effective method for infection detection. Methods: A total of 56 ICU patients with 131 samples were included in this study. The sample types included blood, deep phlegm, urine, drainage, anal swabs, and other types. Samples were analyzed by both conventional detection method and mNGS tests. The diagnosis efficiency and consistency of the two methods were compared. The distribution of the identified pathogens was analyzed. Moreover, the clinical features of patients with mNGS-positive or mNGS-negative results were compared. Results: The positive rate of mNGS was 81.7% (107/131) including 3.1% (4/131) weakly positive, while the positive rate of traditional detection was only 30.5%, including 29 strong positive results and 11 weak positive results. Additionally, there were 41 patients chose to adjust anti-infection strategies according to the results of mNGS, which significantly saved treatment costs. The mNGS-positive patients showed a shorter ICU hospitalization and higher intention to adjust anti-infection strategies than the mNGS-negative patients. Conclusion: mNGS is of great potential for the pathogen detection of ICU patients, and has a higher detection rate than traditional detection methods. Further clinical application investigations can be carried out to expand the application of mNGS.http://www.sciencedirect.com/science/article/pii/S0890850823000075mNGSICUPathogen detectionBloodDeep phlegmUrine |
| spellingShingle | Yanxu Liang Qingguo Feng Kai Wei Xiaoming Hou Xiaotao Song Yuantao Li Potential of metagenomic next-generation sequencing in detecting infections of ICU patients Molecular and Cellular Probes mNGS ICU Pathogen detection Blood Deep phlegm Urine |
| title | Potential of metagenomic next-generation sequencing in detecting infections of ICU patients |
| title_full | Potential of metagenomic next-generation sequencing in detecting infections of ICU patients |
| title_fullStr | Potential of metagenomic next-generation sequencing in detecting infections of ICU patients |
| title_full_unstemmed | Potential of metagenomic next-generation sequencing in detecting infections of ICU patients |
| title_short | Potential of metagenomic next-generation sequencing in detecting infections of ICU patients |
| title_sort | potential of metagenomic next generation sequencing in detecting infections of icu patients |
| topic | mNGS ICU Pathogen detection Blood Deep phlegm Urine |
| url | http://www.sciencedirect.com/science/article/pii/S0890850823000075 |
| work_keys_str_mv | AT yanxuliang potentialofmetagenomicnextgenerationsequencingindetectinginfectionsoficupatients AT qingguofeng potentialofmetagenomicnextgenerationsequencingindetectinginfectionsoficupatients AT kaiwei potentialofmetagenomicnextgenerationsequencingindetectinginfectionsoficupatients AT xiaominghou potentialofmetagenomicnextgenerationsequencingindetectinginfectionsoficupatients AT xiaotaosong potentialofmetagenomicnextgenerationsequencingindetectinginfectionsoficupatients AT yuantaoli potentialofmetagenomicnextgenerationsequencingindetectinginfectionsoficupatients |