The Possible Role of Nitric Oxide and Oxidative Stress in the Enhanced Apoptosis of Cardiac Cells in Cirrhotic Rats
Cirrhosis has been related with hyperdynamic circulation, manifesting as increased cardiac output and decreased systemic vascular resistance. In the present study we examined the cirrhosis outcome on apoptosis of rat hearts. We also tried to explore the role of nitric oxide (NO) and oxidative stress...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2017-02-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/5329 |
| _version_ | 1819123925857075200 |
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| author | Hamed Shafaroodi Mehrdad Hashemi Zahra Nadia Sharif Leila Moezi Zeinab Janahmadi Ahmad Reza Dehpour |
| author_facet | Hamed Shafaroodi Mehrdad Hashemi Zahra Nadia Sharif Leila Moezi Zeinab Janahmadi Ahmad Reza Dehpour |
| author_sort | Hamed Shafaroodi |
| collection | DOAJ |
| description | Cirrhosis has been related with hyperdynamic circulation, manifesting as increased cardiac output and decreased systemic vascular resistance. In the present study we examined the cirrhosis outcome on apoptosis of rat hearts. We also tried to explore the role of nitric oxide (NO) and oxidative stress in the probable changed apoptosis of cirrhotic hearts. Twenty eight days after ligation of bile duct, heart tissues were tested for apoptosis. The extent of malondialdehyde (MDA), and the activities of catalase (CAT), glutathione peroxidase (GSHPx) and superoxide dismutase (SOD) have been calculated in heart tissues. The cirrhotic hearts exhibited structural defects and greater apoptosis. Chronic treatment of cirrhotic rats with L-NAME, a non-selective inhibitor of NO synthase, inhibited heart structural defects and reduced apoptosis of hearts. We also showed that cirrhotic rat hearts had an enhanced level of MDA and reduced activities of CAT, GSHPx and SOD. When the animals were treated by L-NAME chronically, the MDA level reduced and activities of CAT, GSHPx and SOD augmented in cirrhotic heart. In conclusion, increased apoptosis of cirrhotic hearts probably happen due to NO overproduction and increased oxidative stress in hearts of cirrhotic rats. |
| first_indexed | 2024-12-22T07:16:06Z |
| format | Article |
| id | doaj.art-1d060ee02c3d4ceda86438eb26b402ef |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-12-22T07:16:06Z |
| publishDate | 2017-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-1d060ee02c3d4ceda86438eb26b402ef2022-12-21T18:34:23ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942017-02-015515106The Possible Role of Nitric Oxide and Oxidative Stress in the Enhanced Apoptosis of Cardiac Cells in Cirrhotic RatsHamed Shafaroodi0Mehrdad Hashemi1Zahra Nadia Sharif2Leila Moezi3Zeinab Janahmadi4Ahmad Reza Dehpour5Department of Pharmacology and Toxicology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.Department of Molecular Genetics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.Department of Anatomy, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. AND Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. AND The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Cirrhosis has been related with hyperdynamic circulation, manifesting as increased cardiac output and decreased systemic vascular resistance. In the present study we examined the cirrhosis outcome on apoptosis of rat hearts. We also tried to explore the role of nitric oxide (NO) and oxidative stress in the probable changed apoptosis of cirrhotic hearts. Twenty eight days after ligation of bile duct, heart tissues were tested for apoptosis. The extent of malondialdehyde (MDA), and the activities of catalase (CAT), glutathione peroxidase (GSHPx) and superoxide dismutase (SOD) have been calculated in heart tissues. The cirrhotic hearts exhibited structural defects and greater apoptosis. Chronic treatment of cirrhotic rats with L-NAME, a non-selective inhibitor of NO synthase, inhibited heart structural defects and reduced apoptosis of hearts. We also showed that cirrhotic rat hearts had an enhanced level of MDA and reduced activities of CAT, GSHPx and SOD. When the animals were treated by L-NAME chronically, the MDA level reduced and activities of CAT, GSHPx and SOD augmented in cirrhotic heart. In conclusion, increased apoptosis of cirrhotic hearts probably happen due to NO overproduction and increased oxidative stress in hearts of cirrhotic rats.https://acta.tums.ac.ir/index.php/acta/article/view/5329CirrhosisHeartApoptosisNitric oxideOxidative stress |
| spellingShingle | Hamed Shafaroodi Mehrdad Hashemi Zahra Nadia Sharif Leila Moezi Zeinab Janahmadi Ahmad Reza Dehpour The Possible Role of Nitric Oxide and Oxidative Stress in the Enhanced Apoptosis of Cardiac Cells in Cirrhotic Rats Acta Medica Iranica Cirrhosis Heart Apoptosis Nitric oxide Oxidative stress |
| title | The Possible Role of Nitric Oxide and Oxidative Stress in the Enhanced Apoptosis of Cardiac Cells in Cirrhotic Rats |
| title_full | The Possible Role of Nitric Oxide and Oxidative Stress in the Enhanced Apoptosis of Cardiac Cells in Cirrhotic Rats |
| title_fullStr | The Possible Role of Nitric Oxide and Oxidative Stress in the Enhanced Apoptosis of Cardiac Cells in Cirrhotic Rats |
| title_full_unstemmed | The Possible Role of Nitric Oxide and Oxidative Stress in the Enhanced Apoptosis of Cardiac Cells in Cirrhotic Rats |
| title_short | The Possible Role of Nitric Oxide and Oxidative Stress in the Enhanced Apoptosis of Cardiac Cells in Cirrhotic Rats |
| title_sort | possible role of nitric oxide and oxidative stress in the enhanced apoptosis of cardiac cells in cirrhotic rats |
| topic | Cirrhosis Heart Apoptosis Nitric oxide Oxidative stress |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/5329 |
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