Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas
Vegetable oil is an important renewable resource for dietary consumption for human and livestock, and more recently for biodiesel production. Lipid traits in crops are controlled by multiple quantitative trait loci (QTLs) and each of them has a small effect on lipid traits. So far, there is limited...
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| Format: | Article |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01479/full |
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| author | Jian Ye Jian Ye Chunming Wang Chunming Wang Yanwei Sun Yanwei Sun Jing Qu Huizhu Mao Nam-Hai Chua |
| author_facet | Jian Ye Jian Ye Chunming Wang Chunming Wang Yanwei Sun Yanwei Sun Jing Qu Huizhu Mao Nam-Hai Chua |
| author_sort | Jian Ye |
| collection | DOAJ |
| description | Vegetable oil is an important renewable resource for dietary consumption for human and livestock, and more recently for biodiesel production. Lipid traits in crops are controlled by multiple quantitative trait loci (QTLs) and each of them has a small effect on lipid traits. So far, there is limited success to increase lipid yield and improve lipid quality in plants. Here, we reported the identification of a homolog of APETALA2 (AP2) transcription factor WRINKLED1 (JcWRI1) from an oleaginous plant Jatropha curcas and characterized its function in Jatropha and Arabidopsis thaliana. Using physical mapping data, we located JcWRI1 in a QTL region specifying high oleate and lipid content in Jatropha. Overexpression of JcWRI1 in Jatropha elevated seed lipid content and increased seed mass. Lipid profile in seeds of over-expression plants showed higher oleate content which will be beneficial to improve biodiesel quality. Overexpression of JcWRI1 activated lipid-related gene expression and JcWRI1 was shown to directly bind to the AW-box of promoters of some of these genes. In conclusion, we were able to increase seed lipid content and improve seed lipid quality in Jatropha by manipulating one key transcription factor JcWRI1. |
| first_indexed | 2024-04-12T07:50:48Z |
| format | Article |
| id | doaj.art-1d0b5ba127ba4ddb826fb6bedc272ef6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T07:50:48Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1d0b5ba127ba4ddb826fb6bedc272ef62022-12-22T03:41:36ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-10-01910.3389/fpls.2018.01479396241Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcasJian Ye0Jian Ye1Chunming Wang2Chunming Wang3Yanwei Sun4Yanwei Sun5Jing Qu6Huizhu Mao7Nam-Hai Chua8Temasek Life Sciences Laboratory, National University of Singapore, Singapore, SingaporeState Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaTemasek Life Sciences Laboratory, National University of Singapore, Singapore, SingaporeState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agriculture University, Nanjing, ChinaTemasek Life Sciences Laboratory, National University of Singapore, Singapore, SingaporeState Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaTemasek Life Sciences Laboratory, National University of Singapore, Singapore, SingaporeTemasek Life Sciences Laboratory, National University of Singapore, Singapore, SingaporeLaboratory of Plant Molecular Biology, Rockefeller University, New York, NY, United StatesVegetable oil is an important renewable resource for dietary consumption for human and livestock, and more recently for biodiesel production. Lipid traits in crops are controlled by multiple quantitative trait loci (QTLs) and each of them has a small effect on lipid traits. So far, there is limited success to increase lipid yield and improve lipid quality in plants. Here, we reported the identification of a homolog of APETALA2 (AP2) transcription factor WRINKLED1 (JcWRI1) from an oleaginous plant Jatropha curcas and characterized its function in Jatropha and Arabidopsis thaliana. Using physical mapping data, we located JcWRI1 in a QTL region specifying high oleate and lipid content in Jatropha. Overexpression of JcWRI1 in Jatropha elevated seed lipid content and increased seed mass. Lipid profile in seeds of over-expression plants showed higher oleate content which will be beneficial to improve biodiesel quality. Overexpression of JcWRI1 activated lipid-related gene expression and JcWRI1 was shown to directly bind to the AW-box of promoters of some of these genes. In conclusion, we were able to increase seed lipid content and improve seed lipid quality in Jatropha by manipulating one key transcription factor JcWRI1.https://www.frontiersin.org/article/10.3389/fpls.2018.01479/fullJatrophaWRI1QTLlipid contentbiofuel |
| spellingShingle | Jian Ye Jian Ye Chunming Wang Chunming Wang Yanwei Sun Yanwei Sun Jing Qu Huizhu Mao Nam-Hai Chua Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas Frontiers in Plant Science Jatropha WRI1 QTL lipid content biofuel |
| title | Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas |
| title_full | Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas |
| title_fullStr | Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas |
| title_full_unstemmed | Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas |
| title_short | Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas |
| title_sort | overexpression of a transcription factor increases lipid content in a woody perennial jatropha curcas |
| topic | Jatropha WRI1 QTL lipid content biofuel |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01479/full |
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