Trefoil Factor 2 Regulates Proliferation and Apoptosis of Pancreatic Cancer Cells and LPS-Induced Normal Pancreatic Duct Cells by β-Catenin Pathway
Yun Zhou,* Yan Zhang,* Jia Wang Department of Clinical Laboratory Medicine, Shanghai Tenth People’s Hospital of Tongji University, Shanghai 200072, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jia WangDepartment of Clinical Laboratory M...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-10-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/trefoil-factor-2-regulates-proliferation-and-apoptosis-of-pancreatic-c-peer-reviewed-article-CMAR |
| _version_ | 1818317988558798848 |
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| author | Zhou Y Zhang Y Wang J |
| author_facet | Zhou Y Zhang Y Wang J |
| author_sort | Zhou Y |
| collection | DOAJ |
| description | Yun Zhou,* Yan Zhang,* Jia Wang Department of Clinical Laboratory Medicine, Shanghai Tenth People’s Hospital of Tongji University, Shanghai 200072, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jia WangDepartment of Clinical Laboratory Medicine, Tenth People’s Hospital of Tongji University, 301 Yanchang Middle Road, Jingan District, Shanghai 200072, People’s Republic of ChinaTel +86 21-66306756Email wangjiad212@163.comIntroduction: Pancreatic cancer (PC) is a malignant tumor with poor prognosis. This study aimed to determine the role of trefoil factor 2 (TFF2) in the proliferation and apoptosis of LPS-induced normal pancreatic duct cells and pancreatic cancer cells through β-catenin pathway.Methods: TFF2 expression in normal pancreatic duct cells, pancreatic cancer cells and LPS-induced normal pancreatic duct cells was detected by RT-qPCR analysis and Western blot analysis. The transfection effects in pancreatic cancer cells and LPS-induced normal pancreatic duct cells were analyzed by RT-qPCR analysis. After indicated transfection, proliferation, apoptosis and inflammation of these cells were respectively detected by CCK-8 assay, TUNEL assay and certain ELISA kits. Expression of β-catenin pathway-related proteins was analyzed by Western blot analysis. Co-immunoprecipitation assay determined the combination of TFF2 and β-catenin.Results: TFF2 expression was increased in pancreatic cancer cells and LPS-induced HPDE cells compared with HPDE cells. According to TFF2 expression in these cells, PanC-1 cells and 5 μg/mL LPS were selected. In addition, TFF2 interference decreased the proliferation and promoted the apoptosis of PanC-1 cells and LPS-induced HPDE cells. However, TFF2 interference did not obviously change the levels of TNF-α, IL-1β and IL-6 in PanC-1 cells and LPS-induced HPDE cells. Furthermore, TFF2 interference suppressed the expression of β-catenin, c-Myc, Cyclin D1 and BIRC5 in PanC-1 cells and LPS-induced HPDE cells. TFF2 was demonstrated to combine with β-catenin.Discussion: TFF2 interference inhibits proliferation and promotes apoptosis of PanC-1 cells and LPS-induced HPDE cells by suppressing β-catenin pathway.Keywords: trefoil factor 2, pancreatic duct cancer cells, LPS, normal pancreatic duct cells, β-catenin pathway |
| first_indexed | 2024-12-13T09:46:04Z |
| format | Article |
| id | doaj.art-1d0d26d1e04c4767b85806ad6c2a7529 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-13T09:46:04Z |
| publishDate | 2020-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-1d0d26d1e04c4767b85806ad6c2a75292022-12-21T23:52:02ZengDove Medical PressCancer Management and Research1179-13222020-10-01Volume 12107051071358684Trefoil Factor 2 Regulates Proliferation and Apoptosis of Pancreatic Cancer Cells and LPS-Induced Normal Pancreatic Duct Cells by β-Catenin PathwayZhou YZhang YWang JYun Zhou,* Yan Zhang,* Jia Wang Department of Clinical Laboratory Medicine, Shanghai Tenth People’s Hospital of Tongji University, Shanghai 200072, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jia WangDepartment of Clinical Laboratory Medicine, Tenth People’s Hospital of Tongji University, 301 Yanchang Middle Road, Jingan District, Shanghai 200072, People’s Republic of ChinaTel +86 21-66306756Email wangjiad212@163.comIntroduction: Pancreatic cancer (PC) is a malignant tumor with poor prognosis. This study aimed to determine the role of trefoil factor 2 (TFF2) in the proliferation and apoptosis of LPS-induced normal pancreatic duct cells and pancreatic cancer cells through β-catenin pathway.Methods: TFF2 expression in normal pancreatic duct cells, pancreatic cancer cells and LPS-induced normal pancreatic duct cells was detected by RT-qPCR analysis and Western blot analysis. The transfection effects in pancreatic cancer cells and LPS-induced normal pancreatic duct cells were analyzed by RT-qPCR analysis. After indicated transfection, proliferation, apoptosis and inflammation of these cells were respectively detected by CCK-8 assay, TUNEL assay and certain ELISA kits. Expression of β-catenin pathway-related proteins was analyzed by Western blot analysis. Co-immunoprecipitation assay determined the combination of TFF2 and β-catenin.Results: TFF2 expression was increased in pancreatic cancer cells and LPS-induced HPDE cells compared with HPDE cells. According to TFF2 expression in these cells, PanC-1 cells and 5 μg/mL LPS were selected. In addition, TFF2 interference decreased the proliferation and promoted the apoptosis of PanC-1 cells and LPS-induced HPDE cells. However, TFF2 interference did not obviously change the levels of TNF-α, IL-1β and IL-6 in PanC-1 cells and LPS-induced HPDE cells. Furthermore, TFF2 interference suppressed the expression of β-catenin, c-Myc, Cyclin D1 and BIRC5 in PanC-1 cells and LPS-induced HPDE cells. TFF2 was demonstrated to combine with β-catenin.Discussion: TFF2 interference inhibits proliferation and promotes apoptosis of PanC-1 cells and LPS-induced HPDE cells by suppressing β-catenin pathway.Keywords: trefoil factor 2, pancreatic duct cancer cells, LPS, normal pancreatic duct cells, β-catenin pathwayhttps://www.dovepress.com/trefoil-factor-2-regulates-proliferation-and-apoptosis-of-pancreatic-c-peer-reviewed-article-CMARtrefoil factor 2pancreatic duct cancer cellslpsnormal pancreatic duct cellsβ-catenin pathway |
| spellingShingle | Zhou Y Zhang Y Wang J Trefoil Factor 2 Regulates Proliferation and Apoptosis of Pancreatic Cancer Cells and LPS-Induced Normal Pancreatic Duct Cells by β-Catenin Pathway Cancer Management and Research trefoil factor 2 pancreatic duct cancer cells lps normal pancreatic duct cells β-catenin pathway |
| title | Trefoil Factor 2 Regulates Proliferation and Apoptosis of Pancreatic Cancer Cells and LPS-Induced Normal Pancreatic Duct Cells by β-Catenin Pathway |
| title_full | Trefoil Factor 2 Regulates Proliferation and Apoptosis of Pancreatic Cancer Cells and LPS-Induced Normal Pancreatic Duct Cells by β-Catenin Pathway |
| title_fullStr | Trefoil Factor 2 Regulates Proliferation and Apoptosis of Pancreatic Cancer Cells and LPS-Induced Normal Pancreatic Duct Cells by β-Catenin Pathway |
| title_full_unstemmed | Trefoil Factor 2 Regulates Proliferation and Apoptosis of Pancreatic Cancer Cells and LPS-Induced Normal Pancreatic Duct Cells by β-Catenin Pathway |
| title_short | Trefoil Factor 2 Regulates Proliferation and Apoptosis of Pancreatic Cancer Cells and LPS-Induced Normal Pancreatic Duct Cells by β-Catenin Pathway |
| title_sort | trefoil factor 2 regulates proliferation and apoptosis of pancreatic cancer cells and lps induced normal pancreatic duct cells by beta catenin pathway |
| topic | trefoil factor 2 pancreatic duct cancer cells lps normal pancreatic duct cells β-catenin pathway |
| url | https://www.dovepress.com/trefoil-factor-2-regulates-proliferation-and-apoptosis-of-pancreatic-c-peer-reviewed-article-CMAR |
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