Whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep.
The purpose of this investigation was to ascertain whether nitric oxide (NO) released into the circulation by a noninvasive technology called whole body periodic acceleration (WBPA) could increase mucociliary clearance (MCC). It was based on observations by others that nitric oxide donor drugs incre...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0224764 |
| _version_ | 1819259558556598272 |
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| author | Juan R Sabater Marvin A Sackner Jose A Adams William M Abraham |
| author_facet | Juan R Sabater Marvin A Sackner Jose A Adams William M Abraham |
| author_sort | Juan R Sabater |
| collection | DOAJ |
| description | The purpose of this investigation was to ascertain whether nitric oxide (NO) released into the circulation by a noninvasive technology called whole body periodic acceleration (WBPA) could increase mucociliary clearance (MCC). It was based on observations by others that nitric oxide donor drugs increase ciliary beat frequency of nasal epithelium without increasing mucociliary clearance. Tracheal mucous velocity (TMV), a reflection of MCC, was measured in sheep after 1-hour treatment of WBPA and repeated after pretreatment with the NO synthase inhibitor, L-NAME to demonstrated action of NO. Aerosolized human neutrophil elastase (HNE) was administered to sheep to suppress TMV as might occur in cystic fibrosis and other inflammatory lung diseases. WBPA increased TMV to a peak of 136% of baseline 1h after intervention, an effect blocked by L-NAME. HNE reduced TMV to 55% of baseline but slowing was reversed by WBPA, protection lost in the presence of L-NAME. NO released into the circulation from eNOS by WBPA can acutely access airway epithelium for improving MCC slowed in cystic fibrosis and other inflammatory lung diseases as a means of enhancing host defense against pathogens. |
| first_indexed | 2024-12-23T19:11:55Z |
| format | Article |
| id | doaj.art-1d0dbdb5959f41369b05b1140d0724ac |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T19:11:55Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1d0dbdb5959f41369b05b1140d0724ac2022-12-21T17:34:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011411e022476410.1371/journal.pone.0224764Whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep.Juan R SabaterMarvin A SacknerJose A AdamsWilliam M AbrahamThe purpose of this investigation was to ascertain whether nitric oxide (NO) released into the circulation by a noninvasive technology called whole body periodic acceleration (WBPA) could increase mucociliary clearance (MCC). It was based on observations by others that nitric oxide donor drugs increase ciliary beat frequency of nasal epithelium without increasing mucociliary clearance. Tracheal mucous velocity (TMV), a reflection of MCC, was measured in sheep after 1-hour treatment of WBPA and repeated after pretreatment with the NO synthase inhibitor, L-NAME to demonstrated action of NO. Aerosolized human neutrophil elastase (HNE) was administered to sheep to suppress TMV as might occur in cystic fibrosis and other inflammatory lung diseases. WBPA increased TMV to a peak of 136% of baseline 1h after intervention, an effect blocked by L-NAME. HNE reduced TMV to 55% of baseline but slowing was reversed by WBPA, protection lost in the presence of L-NAME. NO released into the circulation from eNOS by WBPA can acutely access airway epithelium for improving MCC slowed in cystic fibrosis and other inflammatory lung diseases as a means of enhancing host defense against pathogens.https://doi.org/10.1371/journal.pone.0224764 |
| spellingShingle | Juan R Sabater Marvin A Sackner Jose A Adams William M Abraham Whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep. PLoS ONE |
| title | Whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep. |
| title_full | Whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep. |
| title_fullStr | Whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep. |
| title_full_unstemmed | Whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep. |
| title_short | Whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep. |
| title_sort | whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep |
| url | https://doi.org/10.1371/journal.pone.0224764 |
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