New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
ABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in t...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Instituto Nacional de Pesquisas da Amazônia
2020-11-01
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| Series: | Acta Amazonica |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305&tlng=en |
| _version_ | 1818988646129205248 |
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| author | Katarine de SOUZA ROCHA Gleiciane SCHUPP DE SENA MESQUITA Maeli Fernanda SILVA FERREIRA Flávia de Nazaré LEITE BARROS Renata Cecília Soares de Lima MACEDO Elane de ARAÚJO SARAIVA Ana Cristina MENDES-OLIVEIRA Valíria DUARTE CERQUEIRA Alessandra SCOFIELD Gustavo GOES CAVALCANTE Isis ABEL Carla Cristina GUIMARÃES DE MORAES |
| author_facet | Katarine de SOUZA ROCHA Gleiciane SCHUPP DE SENA MESQUITA Maeli Fernanda SILVA FERREIRA Flávia de Nazaré LEITE BARROS Renata Cecília Soares de Lima MACEDO Elane de ARAÚJO SARAIVA Ana Cristina MENDES-OLIVEIRA Valíria DUARTE CERQUEIRA Alessandra SCOFIELD Gustavo GOES CAVALCANTE Isis ABEL Carla Cristina GUIMARÃES DE MORAES |
| author_sort | Katarine de SOUZA ROCHA |
| collection | DOAJ |
| description | ABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon. |
| first_indexed | 2024-12-20T19:25:53Z |
| format | Article |
| id | doaj.art-1d0f76248a044539a2be32ba8065ee86 |
| institution | Directory Open Access Journal |
| issn | 0044-5967 |
| language | English |
| last_indexed | 2024-12-20T19:25:53Z |
| publishDate | 2020-11-01 |
| publisher | Instituto Nacional de Pesquisas da Amazônia |
| record_format | Article |
| series | Acta Amazonica |
| spelling | doaj.art-1d0f76248a044539a2be32ba8065ee862022-12-21T19:28:52ZengInstituto Nacional de Pesquisas da AmazôniaActa Amazonica0044-59672020-11-0150430530810.1590/1809-4392201903683New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detectionKatarine de SOUZA ROCHAGleiciane SCHUPP DE SENA MESQUITAMaeli Fernanda SILVA FERREIRAFlávia de Nazaré LEITE BARROSRenata Cecília Soares de Lima MACEDOElane de ARAÚJO SARAIVAAna Cristina MENDES-OLIVEIRAValíria DUARTE CERQUEIRAAlessandra SCOFIELDGustavo GOES CAVALCANTEIsis ABELCarla Cristina GUIMARÃES DE MORAEShttps://orcid.org/0000-0001-8616-648XABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305&tlng=enDidelphimorphiaRodentialive trapskidneyliver16S rRNA |
| spellingShingle | Katarine de SOUZA ROCHA Gleiciane SCHUPP DE SENA MESQUITA Maeli Fernanda SILVA FERREIRA Flávia de Nazaré LEITE BARROS Renata Cecília Soares de Lima MACEDO Elane de ARAÚJO SARAIVA Ana Cristina MENDES-OLIVEIRA Valíria DUARTE CERQUEIRA Alessandra SCOFIELD Gustavo GOES CAVALCANTE Isis ABEL Carla Cristina GUIMARÃES DE MORAES New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection Acta Amazonica Didelphimorphia Rodentia live traps kidney liver 16S rRNA |
| title | New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
| title_full | New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
| title_fullStr | New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
| title_full_unstemmed | New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
| title_short | New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
| title_sort | new records of leptospira spp in wild marsupials and a rodent in the eastern brazilian amazon through pcr detection |
| topic | Didelphimorphia Rodentia live traps kidney liver 16S rRNA |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305&tlng=en |
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