Chromosome stability of in vitro propagated Cucurbita cultivars
Cucurbita pepo L., a member of Cucurbitaceae family, is an annual plant with herbaceous stems, broad leaves and superficial scattered roots. Monoecious flower structure in the Cucurbitaceae family and the differences in the maturing time of male and female organs in flowers cause an increase in the...
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| Format: | Article |
| Language: | English |
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University of Sarajevo, Institute for Genetic Engineering and Biotechnology
2019-12-01
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| Series: | Genetics & Applications |
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| Online Access: | https://genapp.ba/editions/index.php/journal/article/view/65 |
| _version_ | 1811311586187411456 |
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| author | Buse Dursun Ahu Altınkut Uncuoğlu Yıldız Aydin |
| author_facet | Buse Dursun Ahu Altınkut Uncuoğlu Yıldız Aydin |
| author_sort | Buse Dursun |
| collection | DOAJ |
| description |
Cucurbita pepo L., a member of Cucurbitaceae family, is an annual plant with herbaceous stems, broad leaves and superficial scattered roots. Monoecious flower structure in the Cucurbitaceae family and the differences in the maturing time of male and female organs in flowers cause an increase in the foreign fertilization rate. Therefore, there may be positive or negative changes in the existing characteristics of the species. Micropropagation method can be performed in pumpkin species for clonal propagation, but their genetic stability in tissue culture is an important consideration. Chromosome number and morphology are primary cytogenetic parameters that must remain stable after in vitro propagation. We preformed cytogenetic analysis of different hybrid pumpkin genotypes cultivated in our country (Ardendo, Angelina, Torpido, Roni, Sena Hanım) in order to determinate their chromosome stability level. Cotyledon nodes, nodes, shoot apex, hypocotyl and internode explants were prepared from the 4-week old C. pepo seedlings by making a horizontal slice through the hypocotyl region. The highest shoot and callus regeneration was obtained in Torpido genotypes in cotyledonary node explants produced multiple shoots placed in tissue culture media MS+1 mg/l N6-benzylamino-purine BA. The chromosome number and karyotype analysis were determined in control and in vitro propagated Cucurbita pepo L. plants and ploidy levels were confirmed to be 2n = 40.
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| first_indexed | 2024-04-13T10:21:28Z |
| format | Article |
| id | doaj.art-1d103832eae64ed5a7b421af03236498 |
| institution | Directory Open Access Journal |
| issn | 2566-2937 2566-431X |
| language | English |
| last_indexed | 2024-04-13T10:21:28Z |
| publishDate | 2019-12-01 |
| publisher | University of Sarajevo, Institute for Genetic Engineering and Biotechnology |
| record_format | Article |
| series | Genetics & Applications |
| spelling | doaj.art-1d103832eae64ed5a7b421af032364982022-12-22T02:50:29ZengUniversity of Sarajevo, Institute for Genetic Engineering and BiotechnologyGenetics & Applications2566-29372566-431X2019-12-013310.31383/ga.vol3iss3pp25-32Chromosome stability of in vitro propagated Cucurbita cultivarsBuse Dursun0Ahu Altınkut Uncuoğlu1Yıldız Aydin2Marmara University, Faculty of Science and Arts, Department of Biology, Göztepe Campus, Istanbul, TurkeyMarmara University, Faculty of Engineering, Department of Bioengineering, Göztepe Campus, Istanbul, TurkeyMarmara University, Faculty of Science and Arts, Department of Biology, Göztepe Campus, Istanbul, Turkey Cucurbita pepo L., a member of Cucurbitaceae family, is an annual plant with herbaceous stems, broad leaves and superficial scattered roots. Monoecious flower structure in the Cucurbitaceae family and the differences in the maturing time of male and female organs in flowers cause an increase in the foreign fertilization rate. Therefore, there may be positive or negative changes in the existing characteristics of the species. Micropropagation method can be performed in pumpkin species for clonal propagation, but their genetic stability in tissue culture is an important consideration. Chromosome number and morphology are primary cytogenetic parameters that must remain stable after in vitro propagation. We preformed cytogenetic analysis of different hybrid pumpkin genotypes cultivated in our country (Ardendo, Angelina, Torpido, Roni, Sena Hanım) in order to determinate their chromosome stability level. Cotyledon nodes, nodes, shoot apex, hypocotyl and internode explants were prepared from the 4-week old C. pepo seedlings by making a horizontal slice through the hypocotyl region. The highest shoot and callus regeneration was obtained in Torpido genotypes in cotyledonary node explants produced multiple shoots placed in tissue culture media MS+1 mg/l N6-benzylamino-purine BA. The chromosome number and karyotype analysis were determined in control and in vitro propagated Cucurbita pepo L. plants and ploidy levels were confirmed to be 2n = 40. https://genapp.ba/editions/index.php/journal/article/view/65Cucurbita pepo L.in vitro micropropagationkaryotype analysis |
| spellingShingle | Buse Dursun Ahu Altınkut Uncuoğlu Yıldız Aydin Chromosome stability of in vitro propagated Cucurbita cultivars Genetics & Applications Cucurbita pepo L. in vitro micropropagation karyotype analysis |
| title | Chromosome stability of in vitro propagated Cucurbita cultivars |
| title_full | Chromosome stability of in vitro propagated Cucurbita cultivars |
| title_fullStr | Chromosome stability of in vitro propagated Cucurbita cultivars |
| title_full_unstemmed | Chromosome stability of in vitro propagated Cucurbita cultivars |
| title_short | Chromosome stability of in vitro propagated Cucurbita cultivars |
| title_sort | chromosome stability of in vitro propagated cucurbita cultivars |
| topic | Cucurbita pepo L. in vitro micropropagation karyotype analysis |
| url | https://genapp.ba/editions/index.php/journal/article/view/65 |
| work_keys_str_mv | AT busedursun chromosomestabilityofinvitropropagatedcucurbitacultivars AT ahualtınkutuncuoglu chromosomestabilityofinvitropropagatedcucurbitacultivars AT yıldızaydin chromosomestabilityofinvitropropagatedcucurbitacultivars |