Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer
Xubei Ding,1,* Junjun Zheng,2,* Mingxiang Cao3 1Thyroid and Breast Surgery, Jingmen No.1 People’s Hospital, Jingmen, Hubei, People’s Republic of China; 2Pharmacy Intravenous Admixture Services, Jingmen No.2 People’s Hospital, Jingmen, Hubei, People’s Republic...
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Dove Medical Press
2021-01-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/circ0004771-accelerates-cell-carcinogenic-phenotypes-via-suppressing-m-peer-reviewed-article-CMAR |
| _version_ | 1818964902439550976 |
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| author | Ding X Zheng J Cao M |
| author_facet | Ding X Zheng J Cao M |
| author_sort | Ding X |
| collection | DOAJ |
| description | Xubei Ding,1,* Junjun Zheng,2,* Mingxiang Cao3 1Thyroid and Breast Surgery, Jingmen No.1 People’s Hospital, Jingmen, Hubei, People’s Republic of China; 2Pharmacy Intravenous Admixture Services, Jingmen No.2 People’s Hospital, Jingmen, Hubei, People’s Republic of China; 3Department of Anesthesiology, Jingmen No.1 People’s Hospital, Jingmen, Hubei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Mingxiang CaoDepartment of Anesthesiology, Jingmen No.1 People’s Hospital, No. 168, Xiangshan Street, Jingmen, Hubei 448000, People’s Republic of ChinaEmail caomingxiang1984@126.comBackground: Circ_0004771 was demonstrated to mediate cell growth promotion and apoptosis suppression in breast cancer (BC). Herein, the precise functions and mechanism of circ_0004771 in the biological property of BC cells were investigated.Methods: The expression of circ_0004771, microRNA (miR)-1253 and dimethylarginine dimethylaminohydrolase 1 (DDAH1) mRNA was analyzed using quantitative real-time polymerase chain reaction. The proliferation, apoptosis, migration, invasion, adhesion, Western blot and in vivo tumorigenesis assays were employed to evaluate the roles of circ_0004771 and DDAH1 in BC tumorigenesis. The interaction between miR-1253 and circ_0004771 or DDAH1 was validated by dual-luciferase reporter, pull-down and RNA immunoprecipitation (RIP) assay. Exosomes were isolated by Exoquick-TC® methods, and qualified using Nanosight™ technology and Western blot.Results: Circ_0004771 or DDAH1 expression was elevated in BC, and silencing either of them suppressed cell malignant phenotypes, thus impeding BC progression. Importantly, circ_0004771 up-regulation attenuated the anticancer action of DDAH1-knockdown in BC. Additionally, we confirmed that circ_0004771 functioned as a sponge of miR-1253 to up-regulate DDAH1 expression. Moreover, xenograft assay exhibited that circ_0004771 knockdown also hindered tumor growth in vivo via regulating DDAH1 and miR-1253. Besides that, it was found that circ_0004771 was packaged into exosomes isolated from the serum of BC.Conclusion: Circ_0004771 accelerated cell carcinogenic phenotypes via up-regulating DDAH1 expression through absorbing miR-1253 in BC. Besides, circ_0004771 was packaged into exosomes isolated from the serum of BC. All these findings suggested a promising molecular target for BC treatment.Keywords: circ_0004771, miR-1253, DDAH1, breast cancer, exosome |
| first_indexed | 2024-12-20T13:08:29Z |
| format | Article |
| id | doaj.art-1d114215c4b44e3eadd63a2ac555900c |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-20T13:08:29Z |
| publishDate | 2021-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-1d114215c4b44e3eadd63a2ac555900c2022-12-21T19:39:43ZengDove Medical PressCancer Management and Research1179-13222021-01-01Volume 1311160912Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast CancerDing XZheng JCao MXubei Ding,1,* Junjun Zheng,2,* Mingxiang Cao3 1Thyroid and Breast Surgery, Jingmen No.1 People’s Hospital, Jingmen, Hubei, People’s Republic of China; 2Pharmacy Intravenous Admixture Services, Jingmen No.2 People’s Hospital, Jingmen, Hubei, People’s Republic of China; 3Department of Anesthesiology, Jingmen No.1 People’s Hospital, Jingmen, Hubei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Mingxiang CaoDepartment of Anesthesiology, Jingmen No.1 People’s Hospital, No. 168, Xiangshan Street, Jingmen, Hubei 448000, People’s Republic of ChinaEmail caomingxiang1984@126.comBackground: Circ_0004771 was demonstrated to mediate cell growth promotion and apoptosis suppression in breast cancer (BC). Herein, the precise functions and mechanism of circ_0004771 in the biological property of BC cells were investigated.Methods: The expression of circ_0004771, microRNA (miR)-1253 and dimethylarginine dimethylaminohydrolase 1 (DDAH1) mRNA was analyzed using quantitative real-time polymerase chain reaction. The proliferation, apoptosis, migration, invasion, adhesion, Western blot and in vivo tumorigenesis assays were employed to evaluate the roles of circ_0004771 and DDAH1 in BC tumorigenesis. The interaction between miR-1253 and circ_0004771 or DDAH1 was validated by dual-luciferase reporter, pull-down and RNA immunoprecipitation (RIP) assay. Exosomes were isolated by Exoquick-TC® methods, and qualified using Nanosight™ technology and Western blot.Results: Circ_0004771 or DDAH1 expression was elevated in BC, and silencing either of them suppressed cell malignant phenotypes, thus impeding BC progression. Importantly, circ_0004771 up-regulation attenuated the anticancer action of DDAH1-knockdown in BC. Additionally, we confirmed that circ_0004771 functioned as a sponge of miR-1253 to up-regulate DDAH1 expression. Moreover, xenograft assay exhibited that circ_0004771 knockdown also hindered tumor growth in vivo via regulating DDAH1 and miR-1253. Besides that, it was found that circ_0004771 was packaged into exosomes isolated from the serum of BC.Conclusion: Circ_0004771 accelerated cell carcinogenic phenotypes via up-regulating DDAH1 expression through absorbing miR-1253 in BC. Besides, circ_0004771 was packaged into exosomes isolated from the serum of BC. All these findings suggested a promising molecular target for BC treatment.Keywords: circ_0004771, miR-1253, DDAH1, breast cancer, exosomehttps://www.dovepress.com/circ0004771-accelerates-cell-carcinogenic-phenotypes-via-suppressing-m-peer-reviewed-article-CMARcirc_0004771mir-1253ddah1breast cancerexosome |
| spellingShingle | Ding X Zheng J Cao M Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer Cancer Management and Research circ_0004771 mir-1253 ddah1 breast cancer exosome |
| title | Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer |
| title_full | Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer |
| title_fullStr | Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer |
| title_full_unstemmed | Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer |
| title_short | Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer |
| title_sort | circ 0004771 accelerates cell carcinogenic phenotypes via suppressing mir 1253 mediated ddah1 inhibition in breast cancer |
| topic | circ_0004771 mir-1253 ddah1 breast cancer exosome |
| url | https://www.dovepress.com/circ0004771-accelerates-cell-carcinogenic-phenotypes-via-suppressing-m-peer-reviewed-article-CMAR |
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