Advanced Glycation End Products (AGEs) Antibody Protects Against AGEs-induced Apoptosis and NF-ĸB p65 Subunit Overexpression in Rat Glomerular Culture
Advanced glycation end products (AGEs) have been thought to be a major cause of diabetic nephropathy (DN). The mechanisms underlying the involvement of AGEs antibody in diabetic nephropathy are not fully understood. The present study was designed to investigate the protective effect of AGEs antibody...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2016-11-01
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| Series: | Journal of Tropical Life Science |
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| Online Access: | http://jtrolis.ub.ac.id/index.php/jtrolis/article/view/479 |
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| author | Oktavia Rahayu Adianingsih Diana Lyrawati Nur Samsu |
| author_facet | Oktavia Rahayu Adianingsih Diana Lyrawati Nur Samsu |
| author_sort | Oktavia Rahayu Adianingsih |
| collection | DOAJ |
| description | Advanced glycation end products (AGEs) have been thought to be a major cause of diabetic nephropathy (DN). The mechanisms underlying the involvement of AGEs antibody in diabetic nephropathy are not fully understood. The present study was designed to investigate the protective effect of AGEs antibody on AGEs-induced glomerular damage. Isolated glomeruli were pre-incubated either with 10 µg/mL polyclonal anti-AGEs antibody (AGE-pAb) or monoclonal anti-Nɜ -carboxymethyl-lysine antibody (CML-mAb) as a model of AGEs antibody to block interaction of AGEs with receptor for AGEs (RAGE) and incubated afterwards either with 100 µg/mL bovine serum albumin (BSA) or AGE-modified bovine serum albumin (AGE-BSA) for 48 h. Annexin V/nephrin doublestaining was performed to determine apoptosis. Using immunofluorescence, we found that administration of AGE-BSA not only significantly increased glomerular cells apoptosis and nuclear factor kappa B (NF-ĸB) p65 expression, but also reduced expression of nephrin, an important structural and signal molecule of podocytes slit diaphragm. Blocking the interaction of AGE-RAGE with AGEs antibody significantly protected glomerular cells from AGEs-induced apoptosis and NF-ĸB p65 overexpression. We found that AGE-pAb conferred superior protective effect compared with CmL-mAb for the same reduction in apoptosis and NF-ĸB p65 expression. In sharp contrast, CmL-mAb led to preserve expression of podocytes nephrin better than AGE-pAb. These results demonstrate that the antibody against AGEs may be beneficial for preventing the glomerular damage in DN. |
| first_indexed | 2024-03-09T09:02:50Z |
| format | Article |
| id | doaj.art-1d14f90de6f04f4582f20a414f571464 |
| institution | Directory Open Access Journal |
| issn | 2087-5517 2527-4376 |
| language | English |
| last_indexed | 2024-03-09T09:02:50Z |
| publishDate | 2016-11-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-1d14f90de6f04f4582f20a414f5714642023-12-02T11:03:39ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172527-43762016-11-016317618310.11594/jtls.06.03.08186Advanced Glycation End Products (AGEs) Antibody Protects Against AGEs-induced Apoptosis and NF-ĸB p65 Subunit Overexpression in Rat Glomerular CultureOktavia Rahayu Adianingsih0Diana Lyrawati1Nur Samsu2Laboratory of Pharmacy, Faculty of Medicine, Brawijaya University, IndonesiaLaboratory of Pharmacy, Faculty of Medicine, Brawijaya University, IndonesiaDivision of Nephrology and Hypertension, Department of Internal Medicine, Saiful Anwar General Hospital, IndonesiaAdvanced glycation end products (AGEs) have been thought to be a major cause of diabetic nephropathy (DN). The mechanisms underlying the involvement of AGEs antibody in diabetic nephropathy are not fully understood. The present study was designed to investigate the protective effect of AGEs antibody on AGEs-induced glomerular damage. Isolated glomeruli were pre-incubated either with 10 µg/mL polyclonal anti-AGEs antibody (AGE-pAb) or monoclonal anti-Nɜ -carboxymethyl-lysine antibody (CML-mAb) as a model of AGEs antibody to block interaction of AGEs with receptor for AGEs (RAGE) and incubated afterwards either with 100 µg/mL bovine serum albumin (BSA) or AGE-modified bovine serum albumin (AGE-BSA) for 48 h. Annexin V/nephrin doublestaining was performed to determine apoptosis. Using immunofluorescence, we found that administration of AGE-BSA not only significantly increased glomerular cells apoptosis and nuclear factor kappa B (NF-ĸB) p65 expression, but also reduced expression of nephrin, an important structural and signal molecule of podocytes slit diaphragm. Blocking the interaction of AGE-RAGE with AGEs antibody significantly protected glomerular cells from AGEs-induced apoptosis and NF-ĸB p65 overexpression. We found that AGE-pAb conferred superior protective effect compared with CmL-mAb for the same reduction in apoptosis and NF-ĸB p65 expression. In sharp contrast, CmL-mAb led to preserve expression of podocytes nephrin better than AGE-pAb. These results demonstrate that the antibody against AGEs may be beneficial for preventing the glomerular damage in DN.http://jtrolis.ub.ac.id/index.php/jtrolis/article/view/479Advanced glycation end products, antibody, apoptosis, diabetic nephropathy, N e -(carboxymethyl)lysine |
| spellingShingle | Oktavia Rahayu Adianingsih Diana Lyrawati Nur Samsu Advanced Glycation End Products (AGEs) Antibody Protects Against AGEs-induced Apoptosis and NF-ĸB p65 Subunit Overexpression in Rat Glomerular Culture Journal of Tropical Life Science Advanced glycation end products, antibody, apoptosis, diabetic nephropathy, N e -(carboxymethyl)lysine |
| title | Advanced Glycation End Products (AGEs) Antibody Protects Against AGEs-induced Apoptosis and NF-ĸB p65 Subunit Overexpression in Rat Glomerular Culture |
| title_full | Advanced Glycation End Products (AGEs) Antibody Protects Against AGEs-induced Apoptosis and NF-ĸB p65 Subunit Overexpression in Rat Glomerular Culture |
| title_fullStr | Advanced Glycation End Products (AGEs) Antibody Protects Against AGEs-induced Apoptosis and NF-ĸB p65 Subunit Overexpression in Rat Glomerular Culture |
| title_full_unstemmed | Advanced Glycation End Products (AGEs) Antibody Protects Against AGEs-induced Apoptosis and NF-ĸB p65 Subunit Overexpression in Rat Glomerular Culture |
| title_short | Advanced Glycation End Products (AGEs) Antibody Protects Against AGEs-induced Apoptosis and NF-ĸB p65 Subunit Overexpression in Rat Glomerular Culture |
| title_sort | advanced glycation end products ages antibody protects against ages induced apoptosis and nf qb p65 subunit overexpression in rat glomerular culture |
| topic | Advanced glycation end products, antibody, apoptosis, diabetic nephropathy, N e -(carboxymethyl)lysine |
| url | http://jtrolis.ub.ac.id/index.php/jtrolis/article/view/479 |
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