Comparison of a PCR-based Salmonella serotyping kit with traditional serotyping methods
ObjectiveTo evaluate the efficacy of a polymerase chain reaction （PCR）-based rapid serotyping kit， compare it with traditional serological identification methods， and provide a more efficient and convenient method for serological identification of Salmonella.MethodsFifty nine Salmonella strains were...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2022-07-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/202204006?st=article_issue |
| _version_ | 1797976122823016448 |
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| author | DING Fenglan LIU Jiangshan ZHANG Xiaofang QU Lianhai |
| author_facet | DING Fenglan LIU Jiangshan ZHANG Xiaofang QU Lianhai |
| author_sort | DING Fenglan |
| collection | DOAJ |
| description | ObjectiveTo evaluate the efficacy of a polymerase chain reaction （PCR）-based rapid serotyping kit， compare it with traditional serological identification methods， and provide a more efficient and convenient method for serological identification of Salmonella.MethodsFifty nine Salmonella strains were selected from the strain library of our laboratory， and all the strains were typed and identified using the Salmonella serotyping PCR kit and the traditional serum agglutination method， and the coincidence rates of the two typing methods were compared.ResultsAll 59 Salmonella strains were typed by traditional serum agglutination methods， and the typing rate was 100%. All 59 strains were successfully typed by the Salmonella serotyping PCR kit with a typing rate of 100%. The coincidence rate of the two methods was 100%.ConclusionThe Salmonella serotyping PCR kit can achieve a high typing rate， greatly shorten the detection time， reduce the workload of routine serotyping and expensive serum consumption， and save costs. It can be used as a rapid serotyping method for Salmonella application. |
| first_indexed | 2024-04-11T04:46:20Z |
| format | Article |
| id | doaj.art-1d15f2553c914ef3b16a31e64f4978ee |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-11T04:46:20Z |
| publishDate | 2022-07-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-1d15f2553c914ef3b16a31e64f4978ee2022-12-27T09:24:57ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562022-07-0134466867210.13590/j.cjfh.2022.04.0061004-8456（2022）04-0668-05Comparison of a PCR-based Salmonella serotyping kit with traditional serotyping methodsDING Fenglan0LIU Jiangshan1ZHANG Xiaofang2QU Lianhai3Rizhao Center for Disease Control and Prevention, Shandong Rizhao 276826, ChinaBeijing Meizheng Biological Technology Co., Ltd., Beijing 100110, ChinaBeijing Meizheng Biological Technology Co., Ltd., Beijing 100110, ChinaBeijing Meizheng Biological Technology Co., Ltd., Beijing 100110, ChinaObjectiveTo evaluate the efficacy of a polymerase chain reaction （PCR）-based rapid serotyping kit， compare it with traditional serological identification methods， and provide a more efficient and convenient method for serological identification of Salmonella.MethodsFifty nine Salmonella strains were selected from the strain library of our laboratory， and all the strains were typed and identified using the Salmonella serotyping PCR kit and the traditional serum agglutination method， and the coincidence rates of the two typing methods were compared.ResultsAll 59 Salmonella strains were typed by traditional serum agglutination methods， and the typing rate was 100%. All 59 strains were successfully typed by the Salmonella serotyping PCR kit with a typing rate of 100%. The coincidence rate of the two methods was 100%.ConclusionThe Salmonella serotyping PCR kit can achieve a high typing rate， greatly shorten the detection time， reduce the workload of routine serotyping and expensive serum consumption， and save costs. It can be used as a rapid serotyping method for Salmonella application.http://www.zgspws.com/zgspwszz/article/abstract/202204006?st=article_issuesalmonellasalmonella serotypetraditional serum agglutination methodpcr kitmolecular typing |
| spellingShingle | DING Fenglan LIU Jiangshan ZHANG Xiaofang QU Lianhai Comparison of a PCR-based Salmonella serotyping kit with traditional serotyping methods Zhongguo shipin weisheng zazhi salmonella salmonella serotype traditional serum agglutination method pcr kit molecular typing |
| title | Comparison of a PCR-based Salmonella serotyping kit with traditional serotyping methods |
| title_full | Comparison of a PCR-based Salmonella serotyping kit with traditional serotyping methods |
| title_fullStr | Comparison of a PCR-based Salmonella serotyping kit with traditional serotyping methods |
| title_full_unstemmed | Comparison of a PCR-based Salmonella serotyping kit with traditional serotyping methods |
| title_short | Comparison of a PCR-based Salmonella serotyping kit with traditional serotyping methods |
| title_sort | comparison of a pcr based salmonella serotyping kit with traditional serotyping methods |
| topic | salmonella salmonella serotype traditional serum agglutination method pcr kit molecular typing |
| url | http://www.zgspws.com/zgspwszz/article/abstract/202204006?st=article_issue |
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