Evaluation of Cytokeratin 7 Expression in Different Mammary Gland Neoplasms
BACKGROUND: Cytokeratins are non-contractile intermediate filaments engaged in anchoring and structural functions forming a network to support cytoplasm. Cytokeratin 7 (CK7) expression in human breast carcinomas has proved to be a useful differentiation marker, but its expression in canine mammary g...
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| Format: | Article |
| Language: | English |
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University of Tehran
2021-02-01
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| Series: | Iranian Journal of Veterinary Medicine |
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| Online Access: | https://ijvm.ut.ac.ir/article_81250_0b8967f509661bea3686fb8cc819744d.pdf |
| _version_ | 1818893102662811648 |
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| author | Elnaz Elahirad Farhang Sasani Alireza Khosravi Mohammad Javad Gharagozlou Fateme Khanbarari |
| author_facet | Elnaz Elahirad Farhang Sasani Alireza Khosravi Mohammad Javad Gharagozlou Fateme Khanbarari |
| author_sort | Elnaz Elahirad |
| collection | DOAJ |
| description | BACKGROUND: Cytokeratins are non-contractile intermediate filaments engaged in anchoring and structural functions forming a network to support cytoplasm. Cytokeratin 7 (CK7) expression in human breast carcinomas has proved to be a useful differentiation marker, but its expression in canine mammary gland tumors is poorly understood. OBJECTIVES: Cytokeratin 7 (CK7) expression in human breast carcinomas has proved to be a useful differentiation marker, but its expression in canine mammary gland tumors is poorly understood. METHODS: This research was based on the immunohistochemical study of CK7 in 17 cases of canine mammary gland neoplasms obtained from the Department of Pathology, Faculty of Veterinary Medicine, University of Tehran. Masson’s trichrome staining was performed to differentiate between collagen fibers and smooth muscle. RESULTS: CK7 protein was detected in both epithelial (1 benign mixed tumor, 1 fibroadenoma, 1 complex carcinoma, and 1 carcinoma mixed type) and myoepithelial (1 fibroadenoma, 1 benign mixed tumor, 3 complex carcinomas, 1 ductal carcinoma, and 1 carcinoma mixed type) cells. Fine and thick collagen fibers were observed in the sections stained by Masson’s trichrome. CONCLUSIONS: Despite using CK7 as a differentiation marker in human breast cancer, CK7 had a controversial ex-pression in the epithelial and myoepithelial cells in canine mammary gland neoplasms. Based on the results, CK7 could not be considered as an independent marker for the canine mammary glands epithelial cell detection and a prognostic factor in canine mammary gland neoplasms |
| first_indexed | 2024-12-19T18:07:16Z |
| format | Article |
| id | doaj.art-1d178b3f43f64e5b834ef554ea5dd83e |
| institution | Directory Open Access Journal |
| issn | 2251-8894 2252-0554 |
| language | English |
| last_indexed | 2024-12-19T18:07:16Z |
| publishDate | 2021-02-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Iranian Journal of Veterinary Medicine |
| spelling | doaj.art-1d178b3f43f64e5b834ef554ea5dd83e2022-12-21T20:11:25ZengUniversity of TehranIranian Journal of Veterinary Medicine2251-88942252-05542021-02-01151566710.22059/ijvm.2020.295956.100505281250Evaluation of Cytokeratin 7 Expression in Different Mammary Gland NeoplasmsElnaz Elahirad0Farhang Sasani1Alireza Khosravi2Mohammad Javad Gharagozlou3Fateme Khanbarari4Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranMycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranBACKGROUND: Cytokeratins are non-contractile intermediate filaments engaged in anchoring and structural functions forming a network to support cytoplasm. Cytokeratin 7 (CK7) expression in human breast carcinomas has proved to be a useful differentiation marker, but its expression in canine mammary gland tumors is poorly understood. OBJECTIVES: Cytokeratin 7 (CK7) expression in human breast carcinomas has proved to be a useful differentiation marker, but its expression in canine mammary gland tumors is poorly understood. METHODS: This research was based on the immunohistochemical study of CK7 in 17 cases of canine mammary gland neoplasms obtained from the Department of Pathology, Faculty of Veterinary Medicine, University of Tehran. Masson’s trichrome staining was performed to differentiate between collagen fibers and smooth muscle. RESULTS: CK7 protein was detected in both epithelial (1 benign mixed tumor, 1 fibroadenoma, 1 complex carcinoma, and 1 carcinoma mixed type) and myoepithelial (1 fibroadenoma, 1 benign mixed tumor, 3 complex carcinomas, 1 ductal carcinoma, and 1 carcinoma mixed type) cells. Fine and thick collagen fibers were observed in the sections stained by Masson’s trichrome. CONCLUSIONS: Despite using CK7 as a differentiation marker in human breast cancer, CK7 had a controversial ex-pression in the epithelial and myoepithelial cells in canine mammary gland neoplasms. Based on the results, CK7 could not be considered as an independent marker for the canine mammary glands epithelial cell detection and a prognostic factor in canine mammary gland neoplasmshttps://ijvm.ut.ac.ir/article_81250_0b8967f509661bea3686fb8cc819744d.pdfcytokeratin 7dogsmammary neoplasmsmasson’s trichromemyoepithelial cells |
| spellingShingle | Elnaz Elahirad Farhang Sasani Alireza Khosravi Mohammad Javad Gharagozlou Fateme Khanbarari Evaluation of Cytokeratin 7 Expression in Different Mammary Gland Neoplasms Iranian Journal of Veterinary Medicine cytokeratin 7 dogs mammary neoplasms masson’s trichrome myoepithelial cells |
| title | Evaluation of Cytokeratin 7 Expression in Different Mammary Gland Neoplasms |
| title_full | Evaluation of Cytokeratin 7 Expression in Different Mammary Gland Neoplasms |
| title_fullStr | Evaluation of Cytokeratin 7 Expression in Different Mammary Gland Neoplasms |
| title_full_unstemmed | Evaluation of Cytokeratin 7 Expression in Different Mammary Gland Neoplasms |
| title_short | Evaluation of Cytokeratin 7 Expression in Different Mammary Gland Neoplasms |
| title_sort | evaluation of cytokeratin 7 expression in different mammary gland neoplasms |
| topic | cytokeratin 7 dogs mammary neoplasms masson’s trichrome myoepithelial cells |
| url | https://ijvm.ut.ac.ir/article_81250_0b8967f509661bea3686fb8cc819744d.pdf |
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