Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical study
Background: Oleuropein is a potent antioxidant and free-radical scavenger with antiinflammatory properties. Objectives: In the present study, we evaluated the protective effects of oleuropein on myeloperoxidase (MPO) activity, nitrite, urea, creatinine and glomerulosclerosis in alloxan-induced type...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Society of Diabetic Nephropathy Prevention
2017-07-01
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| Series: | Journal of Nephropathology |
| Subjects: | |
| Online Access: | https://nephropathol.com/PDF/jnp-6-204.pdf |
| _version_ | 1827948529697423360 |
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| author | Hassan Ahmadvand Gholamreza Shahsavari Majid Tavafi Shahrokh Bagheri Mohamad Reza Moradkhani Reza Mohammadrezaei Kkorramabadi Peyman Khosravi Maryam Jafari Khadije Zahabi Reza Eftekhar Maryam Soleimaninejad Sanaz Moghadam |
| author_facet | Hassan Ahmadvand Gholamreza Shahsavari Majid Tavafi Shahrokh Bagheri Mohamad Reza Moradkhani Reza Mohammadrezaei Kkorramabadi Peyman Khosravi Maryam Jafari Khadije Zahabi Reza Eftekhar Maryam Soleimaninejad Sanaz Moghadam |
| author_sort | Hassan Ahmadvand |
| collection | DOAJ |
| description | Background: Oleuropein is a potent antioxidant and free-radical scavenger with antiinflammatory properties. Objectives: In the present study, we evaluated the protective effects of oleuropein on myeloperoxidase (MPO) activity, nitrite, urea, creatinine and glomerulosclerosis in alloxan-induced type 1 diabetic rats. Materials and Methods: Thirty Sprague-Dawley male rats were randomly divided into 3 groups: group 1 as control; group 2 as untreated diabetic; and group 3 as treated with oleuropein 15 mg/kg i.p daily. Diabetes was induced in the second and third groups by subcutaneous alloxan injection. After 48 days, the animals were anaesthetized and then the livers and kidneys were removed immediately and used fresh or kept frozen until MPO activity analysis. Blood samples were also collected before sacrificing to measure nitrite, urea, and creatinine. Kidney paraffin sections were prepared to estimate glomerular volume, leukocyte infiltration, and glomerulosclerosis. Results: Oleuropein significantly decreased leukocyte infiltration and glomerulosclerosis in the treated group compared with the diabetic untreated group. Oleuropein significantly decreased the levels of urea, nitrite, and creatinine in the treated group compared with the diabetic untreated group. Moreover, oleuropein significantly decreased MPO activity in the treated group compared with the diabetic untreated group. Conclusions: Oleuropein has antioxidative and antiatherogenic activities and exerts beneficial effects on inflammation and kidney function test and decreases diabetic complication in diabetic rats. |
| first_indexed | 2024-04-09T12:56:46Z |
| format | Article |
| id | doaj.art-1d19d7ffb41542728540d23c595363bc |
| institution | Directory Open Access Journal |
| issn | 2251-8363 2251-8819 |
| language | English |
| last_indexed | 2024-04-09T12:56:46Z |
| publishDate | 2017-07-01 |
| publisher | Society of Diabetic Nephropathy Prevention |
| record_format | Article |
| series | Journal of Nephropathology |
| spelling | doaj.art-1d19d7ffb41542728540d23c595363bc2023-05-13T11:31:23ZengSociety of Diabetic Nephropathy PreventionJournal of Nephropathology2251-83632251-88192017-07-016320420910.15171/jnp.2017.34jnp-20170407120605Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical studyHassan Ahmadvand0Gholamreza Shahsavari1Majid Tavafi2Shahrokh Bagheri3Mohamad Reza Moradkhani4Reza Mohammadrezaei Kkorramabadi5Peyman Khosravi6Maryam Jafari7Khadije Zahabi8Reza Eftekhar9Maryam Soleimaninejad10Sanaz Moghadam11Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khorramabad, IranDepartment of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranDepartment of Anatomy, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranRazi Herbal Researches Center, Lorestan University of Medical Sciences, Khorramabad, IranDepartment of Anesthesia, Madani Heart Center, Lorestan University of Medical Sciences, Khorramabad, Iran.Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khorramabad, IranDepartment of Animal Biology, Payam Noor University, Isfahan, IranStudent Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.Department of Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, IranRazi Herbal Researches Center, Lorestan University of Medical Sciences, Khorramabad, IranBackground: Oleuropein is a potent antioxidant and free-radical scavenger with antiinflammatory properties. Objectives: In the present study, we evaluated the protective effects of oleuropein on myeloperoxidase (MPO) activity, nitrite, urea, creatinine and glomerulosclerosis in alloxan-induced type 1 diabetic rats. Materials and Methods: Thirty Sprague-Dawley male rats were randomly divided into 3 groups: group 1 as control; group 2 as untreated diabetic; and group 3 as treated with oleuropein 15 mg/kg i.p daily. Diabetes was induced in the second and third groups by subcutaneous alloxan injection. After 48 days, the animals were anaesthetized and then the livers and kidneys were removed immediately and used fresh or kept frozen until MPO activity analysis. Blood samples were also collected before sacrificing to measure nitrite, urea, and creatinine. Kidney paraffin sections were prepared to estimate glomerular volume, leukocyte infiltration, and glomerulosclerosis. Results: Oleuropein significantly decreased leukocyte infiltration and glomerulosclerosis in the treated group compared with the diabetic untreated group. Oleuropein significantly decreased the levels of urea, nitrite, and creatinine in the treated group compared with the diabetic untreated group. Moreover, oleuropein significantly decreased MPO activity in the treated group compared with the diabetic untreated group. Conclusions: Oleuropein has antioxidative and antiatherogenic activities and exerts beneficial effects on inflammation and kidney function test and decreases diabetic complication in diabetic rats.https://nephropathol.com/PDF/jnp-6-204.pdfdiabetes mellitusratglomerulosclerosisoleuropeinmyeloperoxidase activitynitric oxidekidney function test |
| spellingShingle | Hassan Ahmadvand Gholamreza Shahsavari Majid Tavafi Shahrokh Bagheri Mohamad Reza Moradkhani Reza Mohammadrezaei Kkorramabadi Peyman Khosravi Maryam Jafari Khadije Zahabi Reza Eftekhar Maryam Soleimaninejad Sanaz Moghadam Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical study Journal of Nephropathology diabetes mellitus rat glomerulosclerosis oleuropein myeloperoxidase activity nitric oxide kidney function test |
| title | Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical study |
| title_full | Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical study |
| title_fullStr | Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical study |
| title_full_unstemmed | Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical study |
| title_short | Protective effects of oleuropein against renal injury oxidative damage in alloxan-induced diabetic rats; a histological and biochemical study |
| title_sort | protective effects of oleuropein against renal injury oxidative damage in alloxan induced diabetic rats a histological and biochemical study |
| topic | diabetes mellitus rat glomerulosclerosis oleuropein myeloperoxidase activity nitric oxide kidney function test |
| url | https://nephropathol.com/PDF/jnp-6-204.pdf |
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