Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children
Asthma is a multifactorial condition that can be associated with obesity. The phenotypes of asthma in lean and obese patients are different, with proinflammatory signatures being further elevated in the latter. Both obesity and asthma are associated with alterations in intestinal barrier function an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/2/866 |
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| author | Samir Córdova Mireia Tena-Garitaonaindia Ana Isabel Álvarez-Mercado Reyes Gámez-Belmonte Mª Amelia Gómez-Llorente Fermín Sánchez de Medina Ana Martínez-Cañavate Olga Martínez-Augustin Carolina Gómez-Llorente |
| author_facet | Samir Córdova Mireia Tena-Garitaonaindia Ana Isabel Álvarez-Mercado Reyes Gámez-Belmonte Mª Amelia Gómez-Llorente Fermín Sánchez de Medina Ana Martínez-Cañavate Olga Martínez-Augustin Carolina Gómez-Llorente |
| author_sort | Samir Córdova |
| collection | DOAJ |
| description | Asthma is a multifactorial condition that can be associated with obesity. The phenotypes of asthma in lean and obese patients are different, with proinflammatory signatures being further elevated in the latter. Both obesity and asthma are associated with alterations in intestinal barrier function and immunity, and with the composition of the intestinal microbiota and food consumption. In this study, we aimed to establish an organoid model to test the hypothesis that the intestinal content of lean and obese, allergic, asthmatic children differentially regulates epithelial intestinal gene expression. A model of mouse jejunum intestinal organoids was used. A group of healthy, normal-weight children was used as a control. The intestinal content of asthmatic obese children differentially induced the expression of inflammatory and mitochondrial response genes (<i>Tnf</i>-tumor necrosis factor, <i>Cd14</i>, <i>Muc13</i>-mucin 13, <i>Tff2</i>-Trefoil factor 2 and <i>Tff3</i>, <i>Cldn1</i>-claudin 1 and <i>5</i>, <i>Reg3g</i>-regenerating family member 3 gamma, <i>mt-Nd1</i>-NADH dehydrogenase 1 and <i>6</i>, <i>and mt-Cyb</i>-mitochondrial cytochrome b) via the RAGE-advanced glycosylation end product-specific receptor, NF-κB-nuclear factor kappa b and AKT kinase signal transduction pathways. Fecal homogenates from asthmatic normal-weight and obese children induce a differential phenotype in intestinal organoids, in which the presence of obesity plays a major role. |
| first_indexed | 2024-03-08T09:53:33Z |
| format | Article |
| id | doaj.art-1d1e3b0e008248d38bce89d9433955f3 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T09:53:33Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1d1e3b0e008248d38bce89d9433955f32024-01-29T13:55:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-0125286610.3390/ijms25020866Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic ChildrenSamir Córdova0Mireia Tena-Garitaonaindia1Ana Isabel Álvarez-Mercado2Reyes Gámez-Belmonte3Mª Amelia Gómez-Llorente4Fermín Sánchez de Medina5Ana Martínez-Cañavate6Olga Martínez-Augustin7Carolina Gómez-Llorente8Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, SpainDepartamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, SpainDepartamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, SpainDepartamento de Farmacología, Facultad de Farmacia, Universidad de Granada, 18071 Granada, SpainIbs.GRANADA, 18012 Granada, SpainIbs.GRANADA, 18012 Granada, SpainUnidad de Pediatría, Hospital Materno-Infantil, 18071 Granada, SpainDepartamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, SpainDepartamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Campus de Cartuja s/n, Universidad de Granada, 18071 Granada, SpainAsthma is a multifactorial condition that can be associated with obesity. The phenotypes of asthma in lean and obese patients are different, with proinflammatory signatures being further elevated in the latter. Both obesity and asthma are associated with alterations in intestinal barrier function and immunity, and with the composition of the intestinal microbiota and food consumption. In this study, we aimed to establish an organoid model to test the hypothesis that the intestinal content of lean and obese, allergic, asthmatic children differentially regulates epithelial intestinal gene expression. A model of mouse jejunum intestinal organoids was used. A group of healthy, normal-weight children was used as a control. The intestinal content of asthmatic obese children differentially induced the expression of inflammatory and mitochondrial response genes (<i>Tnf</i>-tumor necrosis factor, <i>Cd14</i>, <i>Muc13</i>-mucin 13, <i>Tff2</i>-Trefoil factor 2 and <i>Tff3</i>, <i>Cldn1</i>-claudin 1 and <i>5</i>, <i>Reg3g</i>-regenerating family member 3 gamma, <i>mt-Nd1</i>-NADH dehydrogenase 1 and <i>6</i>, <i>and mt-Cyb</i>-mitochondrial cytochrome b) via the RAGE-advanced glycosylation end product-specific receptor, NF-κB-nuclear factor kappa b and AKT kinase signal transduction pathways. Fecal homogenates from asthmatic normal-weight and obese children induce a differential phenotype in intestinal organoids, in which the presence of obesity plays a major role.https://www.mdpi.com/1422-0067/25/2/866organoidsfecal contentgastrointestinal microbiomeasthmaobesity |
| spellingShingle | Samir Córdova Mireia Tena-Garitaonaindia Ana Isabel Álvarez-Mercado Reyes Gámez-Belmonte Mª Amelia Gómez-Llorente Fermín Sánchez de Medina Ana Martínez-Cañavate Olga Martínez-Augustin Carolina Gómez-Llorente Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children International Journal of Molecular Sciences organoids fecal content gastrointestinal microbiome asthma obesity |
| title | Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children |
| title_full | Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children |
| title_fullStr | Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children |
| title_full_unstemmed | Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children |
| title_short | Differential Modulation of Mouse Intestinal Organoids with Fecal Luminal Factors from Obese, Allergic, Asthmatic Children |
| title_sort | differential modulation of mouse intestinal organoids with fecal luminal factors from obese allergic asthmatic children |
| topic | organoids fecal content gastrointestinal microbiome asthma obesity |
| url | https://www.mdpi.com/1422-0067/25/2/866 |
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