A sleep state in Drosophila larvae required for neural stem cell proliferation

Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis...

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Main Authors: Milan Szuperak, Matthew A Churgin, Austin J Borja, David M Raizen, Christopher Fang-Yen, Matthew S Kayser
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-02-01
Series:eLife
Subjects:
Online Access:https://elifesciences.org/articles/33220
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author Milan Szuperak
Matthew A Churgin
Austin J Borja
David M Raizen
Christopher Fang-Yen
Matthew S Kayser
author_facet Milan Szuperak
Matthew A Churgin
Austin J Borja
David M Raizen
Christopher Fang-Yen
Matthew S Kayser
author_sort Milan Szuperak
collection DOAJ
description Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.
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spelling doaj.art-1d1ea218488c4b89921dce3d225e454e2022-12-22T03:52:01ZengeLife Sciences Publications LtdeLife2050-084X2018-02-01710.7554/eLife.33220A sleep state in Drosophila larvae required for neural stem cell proliferationMilan Szuperak0Matthew A Churgin1https://orcid.org/0000-0003-2299-0124Austin J Borja2David M Raizen3https://orcid.org/0000-0001-5935-0476Christopher Fang-Yen4https://orcid.org/0000-0002-4568-3218Matthew S Kayser5https://orcid.org/0000-0003-2359-4967Department of Psychiatry, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesDepartment of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, United StatesDepartment of Psychiatry, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesDepartment of Neurology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States; Chronobiology Program, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States; Center for Sleep and Circadian Neurobiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesDepartment of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, United States; Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesDepartment of Psychiatry, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States; Chronobiology Program, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States; Center for Sleep and Circadian Neurobiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States; Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesSleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.https://elifesciences.org/articles/33220Drosophilasleeplarvaneurogenesisdevelopment
spellingShingle Milan Szuperak
Matthew A Churgin
Austin J Borja
David M Raizen
Christopher Fang-Yen
Matthew S Kayser
A sleep state in Drosophila larvae required for neural stem cell proliferation
eLife
Drosophila
sleep
larva
neurogenesis
development
title A sleep state in Drosophila larvae required for neural stem cell proliferation
title_full A sleep state in Drosophila larvae required for neural stem cell proliferation
title_fullStr A sleep state in Drosophila larvae required for neural stem cell proliferation
title_full_unstemmed A sleep state in Drosophila larvae required for neural stem cell proliferation
title_short A sleep state in Drosophila larvae required for neural stem cell proliferation
title_sort sleep state in drosophila larvae required for neural stem cell proliferation
topic Drosophila
sleep
larva
neurogenesis
development
url https://elifesciences.org/articles/33220
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