A sleep state in Drosophila larvae required for neural stem cell proliferation
Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis...
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eLife Sciences Publications Ltd
2018-02-01
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Series: | eLife |
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Online Access: | https://elifesciences.org/articles/33220 |
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author | Milan Szuperak Matthew A Churgin Austin J Borja David M Raizen Christopher Fang-Yen Matthew S Kayser |
author_facet | Milan Szuperak Matthew A Churgin Austin J Borja David M Raizen Christopher Fang-Yen Matthew S Kayser |
author_sort | Milan Szuperak |
collection | DOAJ |
description | Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation. |
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id | doaj.art-1d1ea218488c4b89921dce3d225e454e |
institution | Directory Open Access Journal |
issn | 2050-084X |
language | English |
last_indexed | 2024-04-12T02:25:12Z |
publishDate | 2018-02-01 |
publisher | eLife Sciences Publications Ltd |
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spelling | doaj.art-1d1ea218488c4b89921dce3d225e454e2022-12-22T03:52:01ZengeLife Sciences Publications LtdeLife2050-084X2018-02-01710.7554/eLife.33220A sleep state in Drosophila larvae required for neural stem cell proliferationMilan Szuperak0Matthew A Churgin1https://orcid.org/0000-0003-2299-0124Austin J Borja2David M Raizen3https://orcid.org/0000-0001-5935-0476Christopher Fang-Yen4https://orcid.org/0000-0002-4568-3218Matthew S Kayser5https://orcid.org/0000-0003-2359-4967Department of Psychiatry, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesDepartment of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, United StatesDepartment of Psychiatry, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesDepartment of Neurology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States; Chronobiology Program, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States; Center for Sleep and Circadian Neurobiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesDepartment of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, United States; Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesDepartment of Psychiatry, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States; Chronobiology Program, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States; Center for Sleep and Circadian Neurobiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States; Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesSleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.https://elifesciences.org/articles/33220Drosophilasleeplarvaneurogenesisdevelopment |
spellingShingle | Milan Szuperak Matthew A Churgin Austin J Borja David M Raizen Christopher Fang-Yen Matthew S Kayser A sleep state in Drosophila larvae required for neural stem cell proliferation eLife Drosophila sleep larva neurogenesis development |
title | A sleep state in Drosophila larvae required for neural stem cell proliferation |
title_full | A sleep state in Drosophila larvae required for neural stem cell proliferation |
title_fullStr | A sleep state in Drosophila larvae required for neural stem cell proliferation |
title_full_unstemmed | A sleep state in Drosophila larvae required for neural stem cell proliferation |
title_short | A sleep state in Drosophila larvae required for neural stem cell proliferation |
title_sort | sleep state in drosophila larvae required for neural stem cell proliferation |
topic | Drosophila sleep larva neurogenesis development |
url | https://elifesciences.org/articles/33220 |
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