Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures
Abstract Background Escherichia coli (E. coli) is the most abundant expression host for recombinant proteins. The production efficiency is dependent on a multitude of parameters. Therefore, high-throughput applications have become an increasingly frequent technique to investigate the main factors. W...
Main Authors: | Martina Mühlmann, Eva Forsten, Saskia Noack, Jochen Büchs |
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Format: | Article |
Language: | English |
Published: |
BMC
2017-11-01
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Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12934-017-0832-4 |
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