Association of Rhizophora mangle and ascorbic acid in hydrogels: Evaluation of cytotoxic and immunomodulatory effects
Abstract Polyphenolics from Rhizophora mangle (R. mangle) have shown wound healing and anti- inflammatory effects that may be potentiated by being associated with ascorbic acid, an important substance for collagen and elastin synthesis that plays a role in tissue repair. In our study, we aimed to fo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2023-11-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502023000100418&tlng=en |
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| author | Eduarda Santos de Santana Leila Bastos Leal Luzia Abílio da Silva Irla Carla de França Barbosa Cristiane Moutinho Lagos de Melo Dayane Kelly Dias do Nascimento Santos Jeymesson Raphael Cardoso Vieira |
| author_facet | Eduarda Santos de Santana Leila Bastos Leal Luzia Abílio da Silva Irla Carla de França Barbosa Cristiane Moutinho Lagos de Melo Dayane Kelly Dias do Nascimento Santos Jeymesson Raphael Cardoso Vieira |
| author_sort | Eduarda Santos de Santana |
| collection | DOAJ |
| description | Abstract Polyphenolics from Rhizophora mangle (R. mangle) have shown wound healing and anti- inflammatory effects that may be potentiated by being associated with ascorbic acid, an important substance for collagen and elastin synthesis that plays a role in tissue repair. In our study, we aimed to formulate an association of R. mangle and ascorbic acid in hydrogels and evaluate the association’s cytotoxic and immunomodulatory effects. In a pre-formulation study, three gelling polymers (i.e.xanthan gum, poloxamer and hydroxyethyl cellulose) were tested. The selected polymer (i.e. xanthan gum) was used to evaluate cytotoxic and immunomodulatory effects using flow cytometry. Xanthan gum (1.5%) had a homogeneous appearance, an orange colour, a smooth surface, intense brightness and the typical odour, as well as non-Newtonian pseudoplastic behaviour. With a pH of 5.0-5.3 and a non-cytotoxic profile, xanthan gum induced the proliferation and activation of CD4 +, CD8+ and NK T lymphocytes and the production of IL- 2, IL-4, IL-10, IL-17 and TNF-α cytokines in stimulated splenocytes. The results suggest that the association of R. mangle and ascorbic acid in 1.5% xanthan gum hydrogel may be promising in preparations for wound-healing processes. |
| first_indexed | 2024-03-11T12:16:24Z |
| format | Article |
| id | doaj.art-1d23ff97267a4a2da5fefbe67d3f179c |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-03-11T12:16:24Z |
| publishDate | 2023-11-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-1d23ff97267a4a2da5fefbe67d3f179c2023-11-07T07:44:28ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902023-11-015910.1590/s2175-97902023e20179Association of Rhizophora mangle and ascorbic acid in hydrogels: Evaluation of cytotoxic and immunomodulatory effectsEduarda Santos de Santanahttps://orcid.org/0000-0002-9019-6072Leila Bastos Lealhttps://orcid.org/0000-0003-3017-9120Luzia Abílio da Silvahttps://orcid.org/0000-0002-8623-3045Irla Carla de França Barbosahttps://orcid.org/0000-0001-8361-403XCristiane Moutinho Lagos de Melohttps://orcid.org/0000-0002-8831-0163Dayane Kelly Dias do Nascimento Santoshttps://orcid.org/0000-0003-2870-1127Jeymesson Raphael Cardoso Vieirahttps://orcid.org/0000-0003-3793-5830Abstract Polyphenolics from Rhizophora mangle (R. mangle) have shown wound healing and anti- inflammatory effects that may be potentiated by being associated with ascorbic acid, an important substance for collagen and elastin synthesis that plays a role in tissue repair. In our study, we aimed to formulate an association of R. mangle and ascorbic acid in hydrogels and evaluate the association’s cytotoxic and immunomodulatory effects. In a pre-formulation study, three gelling polymers (i.e.xanthan gum, poloxamer and hydroxyethyl cellulose) were tested. The selected polymer (i.e. xanthan gum) was used to evaluate cytotoxic and immunomodulatory effects using flow cytometry. Xanthan gum (1.5%) had a homogeneous appearance, an orange colour, a smooth surface, intense brightness and the typical odour, as well as non-Newtonian pseudoplastic behaviour. With a pH of 5.0-5.3 and a non-cytotoxic profile, xanthan gum induced the proliferation and activation of CD4 +, CD8+ and NK T lymphocytes and the production of IL- 2, IL-4, IL-10, IL-17 and TNF-α cytokines in stimulated splenocytes. The results suggest that the association of R. mangle and ascorbic acid in 1.5% xanthan gum hydrogel may be promising in preparations for wound-healing processes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502023000100418&tlng=enRhizophora mangleAscorbic acidHydrogelHealingCytotoxicityImmunological factors. |
| spellingShingle | Eduarda Santos de Santana Leila Bastos Leal Luzia Abílio da Silva Irla Carla de França Barbosa Cristiane Moutinho Lagos de Melo Dayane Kelly Dias do Nascimento Santos Jeymesson Raphael Cardoso Vieira Association of Rhizophora mangle and ascorbic acid in hydrogels: Evaluation of cytotoxic and immunomodulatory effects Brazilian Journal of Pharmaceutical Sciences Rhizophora mangle Ascorbic acid Hydrogel Healing Cytotoxicity Immunological factors. |
| title | Association of Rhizophora mangle and ascorbic acid in hydrogels: Evaluation of cytotoxic and immunomodulatory effects |
| title_full | Association of Rhizophora mangle and ascorbic acid in hydrogels: Evaluation of cytotoxic and immunomodulatory effects |
| title_fullStr | Association of Rhizophora mangle and ascorbic acid in hydrogels: Evaluation of cytotoxic and immunomodulatory effects |
| title_full_unstemmed | Association of Rhizophora mangle and ascorbic acid in hydrogels: Evaluation of cytotoxic and immunomodulatory effects |
| title_short | Association of Rhizophora mangle and ascorbic acid in hydrogels: Evaluation of cytotoxic and immunomodulatory effects |
| title_sort | association of rhizophora mangle and ascorbic acid in hydrogels evaluation of cytotoxic and immunomodulatory effects |
| topic | Rhizophora mangle Ascorbic acid Hydrogel Healing Cytotoxicity Immunological factors. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502023000100418&tlng=en |
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