Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials
There is a strong demand for development of nerve guide conduit with prompt nerve regeneration potential for injury-induced nerve defect. Prior to study on nerve tissue engineering using Schwann cells or nerve stem cells, the effectiveness of photofabricated scaffolds based on photocurable gelatin w...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2004-07-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000004783983639 |
| _version_ | 1819162297243795456 |
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| author | Eduardo Gámez Yoshinobu Goto Kengo Nagata Toru Iwaki Tomio Sasaki Takehisa Matsuda Ph.D. |
| author_facet | Eduardo Gámez Yoshinobu Goto Kengo Nagata Toru Iwaki Tomio Sasaki Takehisa Matsuda Ph.D. |
| author_sort | Eduardo Gámez |
| collection | DOAJ |
| description | There is a strong demand for development of nerve guide conduit with prompt nerve regeneration potential for injury-induced nerve defect. Prior to study on nerve tissue engineering using Schwann cells or nerve stem cells, the effectiveness of photofabricated scaffolds based on photocurable gelatin was examined. This study describes the evaluation of in vivo nerve tissue regeneration potentials of three custom-designed and -fabricated prostheses (inner diameter, 1.2 mm; outer diameter, 2.4 mm; wall thickness, 0.60 mm; and length, 15 mm) made of photocured gelatin: a plain photocured gelatin tube (model I), a photocured gelatin tube packed with bioactive substances (laminin, fibronectin, and nerve growth factor) coimmobilized in a photocured gelatin rod (model II), and a photocured gelatin tube packed with bioactive substances coimmobilized in multifilament fibers (model III). These prostheses were implanted between the proximal and distal stumps 10 mm of the dissected right sciatic nerve of 70 adult male Lewis rats for up to 1 year. The highest regenerative potentials were found using the model III prosthesis, followed by the model II prosthesis. Markedly retarded neural regeneration was observed using the model I prosthesis. These were evaluated from the viewpoints of functional recovery, electrophysiological responses, and tissue morphological regeneration. The significance of the synergistic cooperative functions of multifilaments, which serve as a platform that provides contact guidance to direct longitudinal cell movement and tissue ingrowth and as a cell adhesive matrix with high surface area, and immobilized bioactive substances, which enhance nerve regeneration via biological stimulation, is discussed. |
| first_indexed | 2024-12-22T17:26:00Z |
| format | Article |
| id | doaj.art-1d2723db397945a182504f6ab936f9d3 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-22T17:26:00Z |
| publishDate | 2004-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-1d2723db397945a182504f6ab936f9d32022-12-21T18:18:42ZengSAGE PublishingCell Transplantation0963-68971555-38922004-07-011310.3727/000000004783983639Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration PotentialsEduardo Gámez0Yoshinobu Goto1Kengo Nagata2Toru Iwaki3Tomio Sasaki4Takehisa Matsuda Ph.D.5Divisions of Neurosurgery, Kyushu University, 3-1-1, Maidashi, Higashi-Ku, Fukuoka 812-8582, JapanDivisions of Clinical Neurophysiology, Kyushu University, 3-1-1, Maidashi, Higashi-Ku, Fukuoka 812-8582, JapanDivisions of Graduate School of Medical Sciences and Division of Oral Anatomy and Cell Biology, Graduate School of Dental Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-Ku, Fukuoka 812-8582, JapanDivisions of Neuropathology, and Kyushu University, 3-1-1, Maidashi, Higashi-Ku, Fukuoka 812-8582, JapanDivisions of Neurosurgery, Kyushu University, 3-1-1, Maidashi, Higashi-Ku, Fukuoka 812-8582, JapanDivisions of Biomedical Engineering, Kyushu University, 3-1-1, Maidashi, Higashi-Ku, Fukuoka 812-8582, JapanThere is a strong demand for development of nerve guide conduit with prompt nerve regeneration potential for injury-induced nerve defect. Prior to study on nerve tissue engineering using Schwann cells or nerve stem cells, the effectiveness of photofabricated scaffolds based on photocurable gelatin was examined. This study describes the evaluation of in vivo nerve tissue regeneration potentials of three custom-designed and -fabricated prostheses (inner diameter, 1.2 mm; outer diameter, 2.4 mm; wall thickness, 0.60 mm; and length, 15 mm) made of photocured gelatin: a plain photocured gelatin tube (model I), a photocured gelatin tube packed with bioactive substances (laminin, fibronectin, and nerve growth factor) coimmobilized in a photocured gelatin rod (model II), and a photocured gelatin tube packed with bioactive substances coimmobilized in multifilament fibers (model III). These prostheses were implanted between the proximal and distal stumps 10 mm of the dissected right sciatic nerve of 70 adult male Lewis rats for up to 1 year. The highest regenerative potentials were found using the model III prosthesis, followed by the model II prosthesis. Markedly retarded neural regeneration was observed using the model I prosthesis. These were evaluated from the viewpoints of functional recovery, electrophysiological responses, and tissue morphological regeneration. The significance of the synergistic cooperative functions of multifilaments, which serve as a platform that provides contact guidance to direct longitudinal cell movement and tissue ingrowth and as a cell adhesive matrix with high surface area, and immobilized bioactive substances, which enhance nerve regeneration via biological stimulation, is discussed.https://doi.org/10.3727/000000004783983639 |
| spellingShingle | Eduardo Gámez Yoshinobu Goto Kengo Nagata Toru Iwaki Tomio Sasaki Takehisa Matsuda Ph.D. Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials Cell Transplantation |
| title | Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials |
| title_full | Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials |
| title_fullStr | Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials |
| title_full_unstemmed | Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials |
| title_short | Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials |
| title_sort | photofabricated gelatin based nerve conduits nerve tissue regeneration potentials |
| url | https://doi.org/10.3727/000000004783983639 |
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