Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles
To understand the factors that control the formation of the biomolecular corona, a systematic study of the adsorption of several miRNAs shown to be important in prostate cancer on amine-functionalized mesoporous silica nanoparticles (MSN-NH<sub>2</sub>) has been performed. Process parame...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/5/1196 |
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| author | Carla Vidaurre-Agut Eva María Rivero-Buceta Christopher C. Landry Pablo Botella |
| author_facet | Carla Vidaurre-Agut Eva María Rivero-Buceta Christopher C. Landry Pablo Botella |
| author_sort | Carla Vidaurre-Agut |
| collection | DOAJ |
| description | To understand the factors that control the formation of the biomolecular corona, a systematic study of the adsorption of several miRNAs shown to be important in prostate cancer on amine-functionalized mesoporous silica nanoparticles (MSN-NH<sub>2</sub>) has been performed. Process parameters including miRNA type, nanoparticle concentration, incubation temperature and incubation time were investigated, as well as the potential competition for adsorption between different miRNA molecules. The influence of proteins and particle PEGylation on miRNA adsorption were also explored. We found that low particle concentrations and physiological temperature both led to increased miRNA adsorption. Adsorption of miRNA was also higher when proteins were present in the same solution; reducing or preventing protein adsorption by PEGylating the MSNs hindered adsorption. Finally, the amount of miRNA adsorbed from human serum by MSN-NH<sub>2</sub> was compared to a commercial miRNA purification kit (TaqMan<sup>®</sup>, Life Technologies, Carlsbad, CA, USA). MSN-NH<sub>2</sub> adsorbed six times as much miRNA as the commercial kit, demonstrating higher sensitivity to subtle up- and downregulation of circulating miRNA in the blood of patients. |
| first_indexed | 2024-03-10T11:45:57Z |
| format | Article |
| id | doaj.art-1d2885ebd24940349608922cd1e61391 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T11:45:57Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-1d2885ebd24940349608922cd1e613912023-11-21T18:06:08ZengMDPI AGNanomaterials2079-49912021-05-01115119610.3390/nano11051196Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica NanoparticlesCarla Vidaurre-Agut0Eva María Rivero-Buceta1Christopher C. Landry2Pablo Botella3Instituto de Tecnología Química, Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Avenida de los Naranjos s/n, 46022 Valencia, SpainInstituto de Tecnología Química, Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Avenida de los Naranjos s/n, 46022 Valencia, SpainDepartment of Chemistry, University of Vermont, 82 University Place, Burlington, VT 05405, USAInstituto de Tecnología Química, Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Avenida de los Naranjos s/n, 46022 Valencia, SpainTo understand the factors that control the formation of the biomolecular corona, a systematic study of the adsorption of several miRNAs shown to be important in prostate cancer on amine-functionalized mesoporous silica nanoparticles (MSN-NH<sub>2</sub>) has been performed. Process parameters including miRNA type, nanoparticle concentration, incubation temperature and incubation time were investigated, as well as the potential competition for adsorption between different miRNA molecules. The influence of proteins and particle PEGylation on miRNA adsorption were also explored. We found that low particle concentrations and physiological temperature both led to increased miRNA adsorption. Adsorption of miRNA was also higher when proteins were present in the same solution; reducing or preventing protein adsorption by PEGylating the MSNs hindered adsorption. Finally, the amount of miRNA adsorbed from human serum by MSN-NH<sub>2</sub> was compared to a commercial miRNA purification kit (TaqMan<sup>®</sup>, Life Technologies, Carlsbad, CA, USA). MSN-NH<sub>2</sub> adsorbed six times as much miRNA as the commercial kit, demonstrating higher sensitivity to subtle up- and downregulation of circulating miRNA in the blood of patients.https://www.mdpi.com/2079-4991/11/5/1196mesoporous silica nanoparticlesbiomolecular coronamiRNAmiR-200cmiR-221miR-375 |
| spellingShingle | Carla Vidaurre-Agut Eva María Rivero-Buceta Christopher C. Landry Pablo Botella Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles Nanomaterials mesoporous silica nanoparticles biomolecular corona miRNA miR-200c miR-221 miR-375 |
| title | Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles |
| title_full | Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles |
| title_fullStr | Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles |
| title_full_unstemmed | Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles |
| title_short | Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles |
| title_sort | isolation and quantification of mirna from the biomolecular corona on mesoporous silica nanoparticles |
| topic | mesoporous silica nanoparticles biomolecular corona miRNA miR-200c miR-221 miR-375 |
| url | https://www.mdpi.com/2079-4991/11/5/1196 |
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